Berkala Ilmiah Kimia Farmasi
Vol. 7 No. 1 (2020): JUNI

Inhibitory Activity of Probiotic Milk Against Eschericia coli ATCC 6538 and Staphylococcus aureus ATCC 8739

Andyanita Hanif Hermawati (Program Study of Medical Laboratory Technology, STIKes Hutama Abdi Husada)
Aryati Aryati (Department Patologi Klinik Fakultas Kedokteran Universitas Airlangga)
Isnaeni Isnaeni (Department Kimia Farmasi Fakultas Farmasi Universitas Airlangga)



Article Info

Publish Date
09 Aug 2021

Abstract

The aim of this research is to evaluate inhibitory activity of Lactobacillus acidophilus (L.a) and Lactobacillus casei (L.c) probiotic milk against Escherichia coli and Staphylococcus aureus growth. The fermented milk was prepared by inoculating each probiotic in milk at 43ÂșC and then incubated at room temperature for 24 hours. Diffusion agar method by using nutrient agar media was used to assess the minimum inhibition concentration (MIC). Obtained results showed pH of fermentation probiotic milk 5 + 0,05. Optimum inhibition zone diameter of L. acidophilus fermented milk against Escherichia coli and Staphylococcus aureus at MIC value of 14 mm. Inhibition zone diameter of L. casei fermented milk obtained an optimum against Escherichia coli and Staphylococcus aureus at MIC value of 12 mm and 13 mm respectively. The L. acidophilus and Lactobacillus casei probiotics fermented milk exhibited considerable antibacterial agent against Escherichia coli and Staphylococcus aureus.

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Journal Info

Abbrev

BIKFAR

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Medicine & Pharmacology

Description

to facilitate interaction, discussion, and updating of research ideas in the fields of pharmacy, Pharmaceutical Chemistry, Medicinal Chemistry, Drug Development, Organic synthesis, Analytical chemistry, Pharmaceutical Technology, Pharmaceutical Microbiology and Biotechnology, Pharmacology and ...