Berkala Penelitian Hayati
Vol 25 No 1 (2019): December 2019

Developmental the pattern of embryogenic microspore of rice (Oryza sativa L.) based on morphological characteristic

Siti Nurbaiti (Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)
Yekti Asih Purwestri (Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)
Budi Setiadi Daryono (Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)
Endang Semiarti (Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada)
Ari Indrianto (Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)

Article Info

Publish Date
16 Mar 2020

Abstract

Microspore culture is a rapid approach to get double haploid plants in shorten time which is important for plant breeding. Microspore culture of pigmented rice cultivar Segreng was used to observe the pattern of embryogenic development based on the characteristics of morphology. Characterization between embryogenic and non-embryogenic microspores was conducted after exposed anther to carbohydrate starvation in B medium culture at 33oC for 4 days. The result showed that enlarged microspore and star-like structure of fragmented cytoplasm become a marker of embryogenic microspore after stresses treatment. More complex of fragmented microspore was found at 4 days in A2 medium and developed further into multicellular structure. These multicellular structures might be originated either by symmetrical or asymmetrical division. The longer incubation, the higher frequency of complex fragmented microspore that developed into multicellular structure release from exine has appeared.

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Journal Info
Berkala Penelitian Hayati
Website

Abbrev

jurnal

Publisher

Perhimpunan Biologi Indonesia Cabang Jawa Timur

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Description

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by ...