Berkala Penelitian Hayati
Vol 4 No 2 (1999): June 1999

ISOLASI KARAKTERISASI DAN UJI EKSPRESI GEN ALP1 DI Escherichia coli DH5a

Ni Nyoman Tri Puspaningsih (Biologi, FMIPA, Universitas Airlangga)
Akhmaloka (FMIPA, Instittut Teknologi Bandung)
Sofyan Hadi (Biologi, FMIPA, Universitas Airlangga)
Y. Sri Wulan Manuhara (Biologi, FMIPA, Universitas Airlangga)
Bambang Irawan (Biologi, FMIPA, Universitas Airlangga)

Article Info

Publish Date
30 Jan 2014

Abstract

Amylase gene (ALP1) insertion in YCp 50 cloning vector was detected by Direct Screening PCR. Result of Direct Screening PCR showed one transformants which assumed have amylase gene insert. Characterization of the recombinant DNA by Stu1 and EcoR1 restriction enzymes indicated nucleotide sequence of insert gene. Digestion by BamHI, Cla1, and EcoRV restriction enzymes showed only one band that assumed size of insert gene is about 1500 bp. Gene expression showed that amylase enzyme activity by using Somogyi-Nelson method was 88.0265 Unit. This activity was 10% higher than transformant control (Escherichia coli DH5a which content YCp50).

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Journal Info
Berkala Penelitian Hayati
Website

Abbrev

jurnal

Publisher

Perhimpunan Biologi Indonesia Cabang Jawa Timur

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Description

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by ...