Berkala Penelitian Hayati
Vol 23 No 2 (2018): June 2018

Test of protease activity on pigeon pea beans (Cajanus cajan (L.) Millsp.) fermented by Aspergillus niger

Pujiati Pujiati (Department of Biology Education, Universitas PGRI Madiun, Indonesia)
Cicilia Novi Primiani (Department of Biology Education, Universitas PGRI Madiun, Indonesia)

Article Info

Publish Date
19 Jun 2018

Abstract

The objective of this study is to establish the effect of inoculum concentration and incubation time of protease activity on pigeon pea beans fermented by Aspergillus niger. The study comprises two independent variables: the inoculum concentration of Aspergillus niger, which consists of four levels (0 mL/g; 0.2 mL/g; 0.4 mL/g, and 0.6 mL/g), and six levels of incubation time (0 h, 24 h, 48 h, 72 h, 96 h, and 120 h). The obtained data were tested using two-way analysis of variance (ANOVA) followed by an LSD test with a significance level of 5%. The results show that the inoculum concentration of Aspergillus niger and incubation time do affect the activity of the protease enzyme. The highest enzyme activity of 0.298 U/ml was obtained at an inoculum concentration of Aspergillus niger of 0.4 mL/g, with an incubation time of 96 h (P2K4).

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Journal Info
Berkala Penelitian Hayati
Website

Abbrev

jurnal

Publisher

Perhimpunan Biologi Indonesia Cabang Jawa Timur

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Description

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by ...