Neurologico Spinale Medico Chirurgico
Vol 4 No 3 (2021)

The administration 3% Tamarind (Tamarindus indica) leaves ethanol extract cream inhibited the increase of tyrosinase expression and the amount of skin melanin in male Guinea pigs (Cavia porcellus) skin exposed to UV B

Angelia Ongko Prabowo (Master Program in Biomedical Science, Concentration in Anti-Aging Medicine Faculty of Medicine, Universitas Udayana)
Wimpie Pangkahila (Department of Andrology, Faculty of Medicine, Universitas Udayana, Denpasar, Indonesia)
Ni Putu Sriwidyani (Department of Anatomical Pathology, Faculty of Medicine, Universitas Udayana, Denpasar, Indonesia)



Article Info

Publish Date
13 Dec 2021

Abstract

Background: The main factor of skin aging is exposure to sunlight, especially UV B rays. Tamarind leaves extract known containing tannins, flavonoids, and polyphenols as anti-aging agents. This study was aimed to prove the protective effect of 3% Tamarind leaves (Tamarindus indica) ethanol extract cream in inhibiting the skin aging due to exposure to UV B by evaluate the tyrosinase expression and the amount of melanin. Methods: This was a randomized post-test only control group design involving 36 subjects of male Guinea pigs, that divided into 2 groups. The control group received placebo cream and the treatment group received 3% Tamarind leaves ethanol extract cream. Cream application was given twice a day for 2 weeks. UV B were given 3 times a week for 2 weeks with a total dose of 390 mJ/cm2. Tyrosinase expression was examined by calculating the cell percentage that express the enzyme using the immunohistochemical method. The amount of melanin was examined digitally by calculating the pixel percentage of melanin in Masson Fontana staining slides. Statistical analysis by the Independent T-test to compare the mean between two groups with p=0.05 significance. Results: The mean tyrosinase expression and the mean amount of melanin in the control group was higher than treatment group. 20.650±4.911% vs 16.067±3.451%, p=0.003 and 10.461±4.916 pixel% vs 7.300±3.111 pixel%, p=0.029, respectively. Conclusion: Administration of 3% Tamarind leaves (Tamarindus indica) etanol extract cream inhibited the increase in tyrosinase expression and the amount of skin melanin in male guinea pigs (Cavia porcellus) exposed to UV B.

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Journal Info

Abbrev

nsmc

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience

Description

Neurologico Spinale Medico Chirurgico (NSMC) is an open-access, single-blind peer-reviewed journal, published by Indonesian Neurospine Society (INSS) under the flag of Indonesian Neurosurgery Society (INS) and Faculty of Medicine, Udayana University. NSMC publishes articles which encompass all ...