Journal of Tropical Pharmacy and Chemistry
Vol. 5 No. 2 (2020): Journal of Tropical Pharmacy and Chemistry

Acute Toxicity Assay from Seeds and Flesh of Tarap Fruit (Artocarpus odoratissimus Blanco) Ethanolic Extract against Daphnia magna Larvae

Crissty Magglin (Program Studi Kedokteran, Fakultas Kedokteran, Universitas Mulawarman)
Ika Fikriah (2Laboratorium Farmakologi, Fakultas Kedokteran, Universitas Mulawarman)
Khemasili Kosala (Laboratorium Farmakologi, Fakultas Kedokteran, Universitas Mulawarman)
Hadi Kuncoro (Fakultas Farmasi Universitas Mulawarman , Samarinda, Kalimatan Timur, Indonesia)



Article Info

Publish Date
31 Dec 2020

Abstract

Tarap (Artocarpus odoratissimus Blanco) is one of the plants in the tropics that are consumed by dayak tribe in East Kalimantan. Toxicity tests on seeds and bark have been done but there is no data regarding the acute toxicity of Artocarpus odoratissimus Blanco seeds and flesh of fruit causing the need for acute toxicity tests. This Research to know the acute toxic effects of tarap (Artocarpus odoratissimus Blanco) seed and flesh extracts on larvae of Daphnia magna. Tarap seeds and flesh (Artocarpus odoratissimus Blanco) was taken from dayak market in Samarinda, is East Kalimantan, Indonesia. The seeds and flesh of the tarap fruit are extracted by maceration with ethanol solvent. An acute toxicity test was performed by exposing Dapnia magna larvae aged ? 24 hours with a solution of the experimental group and the control group for 48 hours. Toxicity test results are expressed in percentage of immobilization of larvae of Daphnia magna calculated by probit test to obtain EC50 (Half maximal effective concentration) values. Extracts are toxic if the EC50 value > 1000ppm. EC50 Ethanol extract of tarap seeds obtained values ( 3922,301 ± 324,590 ) for EC50 24h and ( 2964,498 ± 412,498 ) for EC50 48h. The EC50 value of ethanol extract from flesh of tarap fruit is ( 12224.514 ± 2186. 899 ) EC50 24h and ( 6165,235 ± 1940,006 ) EC50 48h.Ethanol extract of tarap fruit and flesh is non toxic to larvae of Daphnia magna.

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Journal Info

Abbrev

jtpc

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Medicine & Pharmacology Public Health

Description

Journal of Tropical Pharmacy and Chemistry is a Biannual, international, open access (Indexed in DOAJ, Crossref, SINTA, PKP Index, BASE, Moraref, Google Scholar), journal dedicated to various disciplines of pharmaceutical and allied sciences. Journal of Tropical Pharmacy and Chemistry (ISSN-p: ...