Tapak dara or Madagascar Periwinkle (Catharanthus roseus [L] G.Don), a natural plant, is empirically reported to have promising anticancer activity. To elucidate its mechanism, a research was conducted to investigate the possible ethanol extract ofàC.àroseus in inducing apoptosis on breast cancer cell line (T47D). Antioxidant activity of C. roseus was investigated as well. Sub-G1 flowcytometric apoptotic analysis result showed that extract of C. roseus at 6.25 üg/mL induced apoptosis for 26.365%. Increasing extract concentration resulted an increasing apoptotic level as well, extract at concentration of 12.5 üg/mL induced apoptosis for 22.235%. àMeanwhile doxorubicin at concentration of 10àüg/mL induced apoptosis for 36.055%. The antioxidant activity was determined by usingàin vitro assay: inhibition ofà2,2-diphenyl-1-picrylhydrazyl (DPPH) scavengingàactivity. Antioxidant activity ofàC. roseus extract were compared to quercetin and butylated hydroxyanisol (BHA), as positive controls.àThe results showed that DPPH IC50 of C. roseus extract, quercetin and BHA were 358.411 üg/mL, 19.200 üg/mLàandà94.178àüg/mL, respectively. We suggest thatàC. roseus extract had a potential anticancer activity by inducing apoptosis.
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