<![CDATA[For more than four decades, combination chemotherapy (co-chemotherapy) has been employed as a means to increase the effectiveness of chemotherapy regiments. The aim of our research is to investigate the activity ofàMoringa oleiferaàL. (tanaman kelor) ethanolic extract (MEE) as a co-chemotherapy agent with 5-fluorouracil (5-FU) on WiDr colon cancer cell line. Evaluation of MEE potency as a co-chemotherapy agent with 5-FU was based on cytotoxic activity based on percent cell viability via MTTàassay, and based on apoptosis observation via the double staining method using acrydin orange ââ¬â ethidium bromide (AE) as the staining reagent.Cytotoxicity evaluation of single treatment using concentrations of 5, 20, 50, 100,125, and 250 õg/ml of MEE reduced cell viability 24 hours post-treatment. 5, 50, and 250 õg/ml of MEE was chosen as the combination concentrations with 1000 õM 5-FU. MTT assay 24 hours and 48 hours post-combination treatment showed significant cell viability reduction in comparison to those of single treatments. Apoptosis observation using the double staining method shows the presence of apoptotic cells 48 hours post combination treatment. MEE is a potential co-chemotherapy agentàby increasing the sensitivityàof WiDr colon cancer cell line towards 5-FU.]]>
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