Physalis angulataàL.àisàanàannualàherbàwidelyàusedàasàpopularàmedicineàforàthe treatment of cancer. Physalis angulata L. ethanolic extract (PEE) has been demonstrated to have strong cytotoxic activity against breast cancer, inhibited cancer cellââ¬â¢s proliferation and inducedàcellàcycleàarrest.àTheàaimàofàouràstudyàisàtoàinvestigateàtheàeffectàofàPEEàasàa canceràchemopreventiveàagentàonà7,12-dimethylbenz[a]nthraceneà(DMBA)-inducedàrats mammary. The antiproliferative activity was characterized by monitoring the histopatology representationàandàexpressionàofàcellàproliferationàonàDMBA-inducedàmammaryàratsàthat wereàtreatedàwithàPEEàagainstàcontrolàgroups.àTheàhistopatologyàrepresentationàwere analyzedàbyàHaematoksilinàEosinà(HE)àstainingàmethod,àwhileàproliferativeàactivityàwas detected by AgNOR method. The HE staining results showed significant differences in cells morphologyàofàtreatmentàgroupsàcomparedàtoàtheàcontrolàgroups.àThusàresultsàsuggest thatàPEEàwasàableàtoàrepairàmorphologyàofàcellsàundergoingàcarcinogenesis.àAgNOR methodàshowedàdecreasingàoccurrenceàofàblackàdotsàbetweenàtreatmentàandàcontrol groups. Thus, we conclude that PEE has an antiproliferative activity on DMBA-induced rat mammary.àTherefore,àtheàethanolicàextractàofàPhysalisàangulataàherbsàisàaàpotential chemopreventive agent on cancer. Further study on its molecular mechanism needs to be explored. Keywords:àPhysalisàangulata,àbreastàcancer, 7,12-dimethylbenz[a]nthracene, carcinogenesis, antiproliferative
Copyrights © 2011