AGRIVITA, Journal of Agricultural Science
Vol 44, No 1 (2022)

Sensitivity in Callus Tissue of Plectranthus amboinicus (L.) through Mutation Induction with Colchicine

Kunto Wibisono (Department of Agronomy and Horticulture, Faculty of Agronomy, Bogor Agricultural University (IPB University), Bogor 16680, Indonesia Banten Assessment Institute for Agricultural Technology, Indonesian Agency for Agricultural Research and Development,)
Syarifah Iis Aisyah (Department of Agronomy and Horticulture, Faculty of Agronomy, Bogor Agricultural University (IPB University), Bogor 16680, Indonesia)
Waras Nurcholis (Department of Biochemistry, Faculty of Mathematics and Natural Science, Bogor Agricultural University (IPB University), Bogor 16680, Indonesia Tropical Biopharmaca Research Center, Bogor Agricultural University (IPB University), Bogor 16680, Indonesi)
Sri Suhesti (Indonesian Center for Estate Crops Research and Development, Ministry of Agriculture, Bogor 16111, Indonesia)



Article Info

Publish Date
24 Dec 2021

Abstract

Mutation induction by colchicine is one method to improve plant genetic diversity (polyploidy). This study aims to 1) obtain growth regulators composition suitable in inducing callus, 2) obtain the LC50 value in callus of P. amboinicus, 3) to determine the effect of colchicine on callus growth, 4) to get shoots (putative mutant) varian, regenerating from indirect organogenesis (callus) in MV1. The study is conducted at the Laboratory of Agricultural Seed Management, Plantation Research, and Development Center from June 2018 to June 2019. The study uses a complete randomized design with four treatments and three replications. Explants use ware the nodes. Callus induction uses MS0 medium with 0.5 ppm 2.4-D, 2 ppm 2.4-D, 0.5 ppm 2.4-D + 1 ppm BAP, and 0.5 ppm 2.4-D + 0.5 ppm TDZ treatment. Mutation induction was performed using colchicine concentration at 0%, 0.02%, 0.04%, and 0.06% treatments. The regeneration media is an MS0 medium. The results show the best combination of plant growth regulators to induce the callus is 0.5 ppm 2.4-D. The LC50 colchicine concentration is at 0.0275%. However, this phenotype is reversible. An additional subculture period is required to move to normal morphology

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Journal Info

Abbrev

AGRIVITA

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

AGRIVITA Journal of Agricultural Science is a peer-reviewed, scientific journal published by Faculty of Agriculture Universitas Brawijaya Indonesia in collaboration with Indonesian Agronomy Association (PERAGI). The aims of the journal are to publish and disseminate high quality, original research ...