Jurnal Mangifera Edu
Vol 6 No 2 (2022): Jurnal Mangifera Edu

Various Concentration Distribution of Benzil Amino Purine Concentration on Callus Growth in Apple Cucumber Originated from Karawang

Liga Nursolihah (Fakultas Pertanian, Universitas Singaperbangsa Karawang)
Slamet Abadi (Universitas Singaperbangsa Karawang)
Nurcahyo Widyodaru Saputro (Universitas Singaperbangsa Karawang)



Article Info

Publish Date
31 Jan 2022

Abstract

The purpose of the study is to find the best Benzil Amino Purine (BAP) concentrations and proper explants for the growth of the apple cucumber callus (Cucumis sp.) on Murashige and Skoog (MS) media. The research method used was an experimental method with nonparametric statistics with treatments A0B1 (BAP 0 + Leaves), A0B2 (BAP 0 + Stem), A1B1 (BAP 0.5 μM + Leaf), A1B2 (BAP 0.5 μM + Stem), A2B1 (BAP 1.5 μM + Leaf), A2B2 (BAP 1.5 μM + Stem), A3B1 (BAP 3.0 μM+ Leaves) and A3B2 (BAP 3.0 μM+ Stems) and analyzed descriptively using the Kruskal Wallis Test. The parameters observed were callus emergence, callus diameter, growth rate and callus morphology. The results showed accretion of an apple cucumber callus at concentration 0.5 μM BAP + stem which exerted a faster effect on callus formation time (27 hsi), 1.5 μM BAP + stem giving the largest diameter with 1.2 cm. The stem explant gives the best growth rate (0.8 cm/3 day) as well as concentrations of 0.5 μM and 1.5 μM giving the best growth rate (0.462 cm/3 day and 0.887 cm/3 day). This result showed calluses A0B2 and A1B1 (compact + green) as well as A1B2 and A2B2 (crumb + yellowish-green).

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Journal Info

Abbrev

mangiferaedu

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Library & Information Science

Description

Mangifera Edu journal is a scientific papers on the results of biology and biology education wich managed by Biology education departement, FKIP Wiralodra University. This journal published since 2016 with p-ISSN 2527-9939 and avaliable electronic journal since 2018 with number e-ISSN 2622-3384. The ...