Nusantara Science and Technology Proceedings
Sains dan Teknologi Pertanian Modern

Pathogenicity of Entomopathogenic Nematodes (NEP) Results of In Vitro Propagation Using Egg Yolk Media Against Mustard Caterpillar Plutella xylostella

Mohamad Hipti (Agrotechnology Study Program, Agriculture Faculty, Universitas Pembangunan Nasional“Veteran” Jawa Timur, Surabaya 60294, Indonesia)
Wiludjeng Widajati (Agrotechnology Study Program, Agriculture Faculty, Universitas Pembangunan Nasional“Veteran” Jawa Timur, Surabaya 60294, Indonesia)
Sri Wiyatiningsih (Agrotechnology Study Program, Agriculture Faculty, Universitas Pembangunan Nasional“Veteran” Jawa Timur, Surabaya 60294, Indonesia)
Ramadhani Mahendra Kusuma (Agrotechnology Study Program, Agriculture Faculty, Universitas Pembangunan Nasional“Veteran” Jawa Timur, Surabaya 60294, Indonesia)



Article Info

Publish Date
07 Dec 2021

Abstract

Decreased mustard plant production is inseparable from the presence of plant pests and disease, one of which is Plutella xylostella. This pest can reduce the yield by 30-40%, even in some cases found to experience crop failure. Generally, in the community, controlling pests is using chemical pesticides. However, excessive use of chemical pesticides harms the environment and triggers health problems in humans, So, biological control is needed. One of them is an entomopathogenic nematode (NEP). Advantages Nematodes are environmentally friendly and are fast in crippling their host, but the multiplication of entomopathogenic nematodes encounters many obstacles, so research on these alternatives is needed. This research was carried out to determine the pathogenicity of entomopathogenic Nematodes cultured from the egg yolk media on Plutella xylostella larvae, then developed as an effective biocontrol. The pathogenicity test for Plutella xylostella larvae was performed using nine treatments placed in a factorial Completely Randomized Design (CRD) with each treatment repeated three times. The study indicate that the four bacterial isolates had the same ability to kill Plutella xylostella larvae during four days of observation, with the greatest percentage of 100 percent and the lowest percentage of 76.7 percent.

Copyrights © 2021






Journal Info

Abbrev

nuscientech

Publisher

Subject

Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology

Description

NST Proceeding supports regional research communities to globalise their findings in Science and Technology by providing an open access, online platform in line with international publishing standards and indexing scholarly conference proceedings. The current emphasis of the NST Proceeding includes ...