This studyaims at determine the interactionbetween the different level of 2.4-D andTDZ concentrations, to induction theembryogeniccallus of physic nut. This Research used completely randomized design with factorial treatment pattern, which consists oftwofactors :concentration of2,4 –D, which consists of 5levels : 0.0, 2.5, 5.0, 7.5, 10.0 ppm, and the concentration ofTDZconsists of 5levels: 0.0, 0.5, 1.0, 1.5, 2.0 ppm. The results showes that there are interactions between 2,4-D andTDZ to formingcallus ofthe apicalbudexplants of physic nut.The combination of5.0 ppm2,4-D + 1.0ppmTDZand7.5ppm2,4-D + 1.5ppmTDZproducedthe time appearing of callusfastestcompared toother treatments. The highestpercentage ofcallus formation occuredin thetreatment of2.5ppm2,4-D + 1.0ppmTDZand7.5ppm2,4-D + 1.5ppmTDZ. Whiteyellow color, shinyand friable are characteristicof embryogenic callusproduced on thetreatment of0.0 ppmand 2.5ppm2,4-D with the addition ofTDZata concentration of 0.5ppm, 1.0 ppm, 1.5 ppmand 2.0ppm. Key words : embryogenic, callus, TDZ
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