journal of Basic Medical Veterinary
Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022

Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya

Amirul Muslim Amrullah (Program Profesi Dokter Hewan, Fakultas Kedokteran Hewan, Universitas Airlangga)
Fedik Abdul Rantam (Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga)
Dadik Raharjo (Departemen Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga)
Wiwiek Tyasningsih (Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga)
Agnes Theresia Soelih Estoepangestie (Departemen Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga)
Martia Rani Tacharina (Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga)

Article Info

Publish Date
01 Jul 2022

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine’s Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).

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Journal Info
journal of Basic Medical Veterinary
Website

Abbrev

JBMV

Publisher

Universitas Airlangga

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Description

This journal published original articles, review articles, and case studies in Indonesian or English, in the scope of JBMV has a broad coverage of relevant topics across veterinary basic medical sciences which includes: preclinical and paraclinical disciplines like Anatomy, Physiology, Biochemistry, ...