Makara Journal of Technology
Vol. 20, No. 2

Sugarcane Bagasse as a Carrier for the Immobilization of Saccharomyces cerevisiaein Bioethanol Production

Anita, Sita Heris (Unknown)
Mangunwardoyo, Wibowo (Unknown)
Yopi, Yopi (Unknown)

Article Info

Publish Date
02 Aug 2016


Sugarcane bagasse was used as a carrier to immobilize Saccharomyces cerevisiae in bioethanol production. This research aims to study the potential use of sugarcane bagasse as an alternative carrier for cell immobilization and improvement in the production process of cell immobilization in bagasse. The results showed that the physical characteristics of sugarcane bagasse as a carrier were water content (7.77 ± 0.35%), water retention (4.80 ± 0.44 g/g), water absorption index (8.58 ± 0.22 g/g), and lignin content (24.40 ± 1.52 %). Determination of cell retention was performed in an inoculum volume of 50 mL yeast suspension with various carrier weights (2.5, 5, 10, and 20 g). The highest cell retention was obtained in ratio of 2.5 g carrier/50 mL cell suspension with cell retention of 5.41 ± 1.06 mg/g, or known as biocatalyst. Biocatalyst, as much as 1.5, 3, 4.5, and 6 g, were used as inoculum for a 24 hour bioethanol fermentation. The best concentration and productivity of bioethanol, obtained by using 3 g of biocatalyst, were 23.95 ± 0.28 g/L and 1.24 ± 0.01 g/L/hours. The average of bioethanol yield for a 24 hour fermentation by using immobilized cells was three times higher than the free cells system.

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Journal Info





Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Materials Science & Nanotechnology Mechanical Engineering


MAKARA Journal of Technology is a peer-reviewed multidisciplinary journal committed to the advancement of scholarly knowledge and research findings of the several branches of Engineering and Technology. The Journal publishes new results, original articles, reviews, and research notes whose content ...