Biomedical Engineering
Vol 1, No 1 (2014)

Phytochemical assay, potential of antimalarial and antioxidant activities of green tea extract and its fractions

Fanny Rahardja (Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia)
Rita Tjokropranoto (Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia)
Wahyu Widowati (Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia)
Siska Lusiana (Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia)
Adrian Suhendra (Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia)
Susy Tjahjani (Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia)
Iwan Budiman (Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia)
Maesaroh Maesaroh (Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia)
Nurul Fauziah (Bimolecular and Biomedical Research Center, Aretha MedikaUtama, Bandung, West Java, Indonesia)



Article Info

Publish Date
09 Sep 2014

Abstract

Objective : The research objective was to evaluate the phytochemical compounds, the antioxidant and anti-malarial activity of green tea extract and its fraction. Methods: The antioxidant activity were measured by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity used in vitro laboratories prospective experimental design and completely randomized design, using ethanol extract, hexane, ethyl acetate, butanol fraction, and water fraction ofgreen tea in 7 levels of concentration compared to epicatechin (EC). The data were analyzed using linear regression analysis, and were continued by determined Inhibitory Concentration). The anti-malarial activity of green tea fractions were compared to artemisinin. The data were analyzed using probit analysis, and were continued by determined IC. Results: The results showed that the highest antioxidant activity (IC50) was the fraction of ethyl acetate 2,184 µg/ml. The anti-malarial activity IC50 50 of water and ethyl acetate fraction, ethanol extract and butanol fraction were 0,000090 µg/mL,0,003 µg/mL, 0,006 µg/mL, 0,011 µg/ml respectively.Conclusion: Extract and fractions of green tea have potential anti-malarial and antioxidant properties. Key Words: antioxidant; anti-malarial; green tea; free radicals

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