Jurnal Teknologi Pertanian
Vol 8, No 1 (2007)

Extraction and Characterization of Endogenous Polygalacturonase Enzyme in Cocoa Pulp

Gandaputra, G. P. (Unknown)
Harijono, Harijono (Unknown)
Susanto, Tri (Unknown)
Kumalaningsih, Sri (Unknown)
Aulanni'am, Aulanni'am (Unknown)

Article Info

Publish Date
24 Jan 2012

Abstract

Endogenous polygalacturonase (PG) enzymes in cocoa pulp have not been studied yet. The research, aimed to extract and characterize endogenous PG enzymes in cocoa pulp, was carried in two steps: (i) extraction of endogenous PG enzyme and (ii) enzyme characterization i.e. enzyme activity assay and determination of enzymatic kinetics (Km and Vmax), molecular weight (MW) by electrophoresis, and determination of its optimum temperature and pH. The results showed that the characteristics of endogenous PG in cocoa pulp were as followed: the relative activity of 4,58± 0,06 Units/ml,  Vmax of 6,69 Units/ml, Km of 0,37 %, MW was 32,84 and 63,58 kDa, and the combination of temperature and pH optimum were 42,5oC and 4,6. Key words: endogenous polygalacturonase (PG), cocoa pulp

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Journal Info
Jurnal Teknologi Pertanian
Website

Abbrev

JTP

Publisher

Universitas Brawijaya

Subject

Agriculture, Biological Sciences & Forestry Computer Science & IT

Description

Jurnal Teknologi Pertanian diterbitkan oleh Fakultas Teknologi Pertanian Universitas Brawijaya untuk penyebarluasan hasil penelitian yang dilakukan oleh para peneliti dari dalam dan luar Fakultas Teknologi Pertanian Universitas Brawijaya. Jurnal Teknologi Pertanian terbit tiga kali dalam setahun, ...