AGRIVITA, Journal of Agricultural Science
Vol 37, No 2 (2015)

OBTAINING Artemisia cina POLYPLOIDY THROUGH PLANT GROWTH REGULATOR TREATMENT IN SHOOT CULTURE

Herawati, Maria Marina ( Satya Wacana Christian University)
Pudjihartati, Endang (Unknown)
Pramono, Suwijiyo (Unknown)
Sulistyaningsih, Endang (Unknown)
Purwantoro, Aziz (Unknown)



Article Info

Publish Date
27 May 2015

Abstract

Artemisia cina is a medicinal plant species which produces bioactive compound potential to anti-tumor, antifungal and antibacterial medicines. This study was aimed to obtain A. cina polyploid plants through a treatment of growth regulators in shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L-1 of 2,4-dichloro phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L-1 of Benzyladenine (BA) for 28 days. Chromosome analysis showed that the highest polyploidy percentage, 23%, was reached in the treatment of 2 mg L-1 of 2,4-D combined with 1 of BA mg L-1. The polyploidy level varied, comprising 2n=3x, 2n=4x, 2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area, larger stomatal size, and higher chlorophyll content than diploid plants. However stomatal density of polyploidy plants was lower than that of in diploid plants.Keywords: 2,4-D, Artemisia cina, BA, polyploidy

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Journal Info

Abbrev

AGRIVITA

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

AGRIVITA Journal of Agricultural Science is a peer-reviewed, scientific journal published by Faculty of Agriculture Universitas Brawijaya Indonesia in collaboration with Indonesian Agronomy Association (PERAGI). The aims of the journal are to publish and disseminate high quality, original research ...