Proceeding of International Conference Health, Science And Technology (ICOHETECH)
Proceeding of the 1st International Conference Health, Science And Technology (ICOHETECH)

N-(2-chlorobenzyl) formamide, a Novel Synthesized Antituberculosis Evaluation by Microplate Alamar Blue Assay

Desy Ayu Irma Permatasari (Universitas Gadjah Mada)
Ritmaleni (Universitas Gadjah Mada)



Article Info

Publish Date
16 Nov 2019

Abstract

The multicomponent amidoalkylation reaction is done by mixing three compounds simultaneously. This method develops in the area of anti-tuberculosis. Tuberculosis (TB) is an infectious disease caused by M. tuberculosis. A novel N-(2-chlorobenzyl) formamide was synthesized by reacted with formic acid or NaBH4. The synthesized compounds were identified by structural elucidation using spectroscopic methods (1H-NMR, 13C-NMR, FTIR) and GC-MS. The antituberculosis activity test used the Microplate Alamar Blue Assay (MABA) method with a concentration series of 1000-1,954 µg / mL against M. tuberculosis bacteria strain H37Rv. The results showed that the product was successfully synthesized using a multicomponent reaction with formic acid. N-(2-chlorobenzyl)formamide has MIC value 1000 μg / mL for its evaluation on antituberculosis activity with MABA method but are less potent than INH (2 μg / mL) as drug control.

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