PREVENIRE: Journal of Multidisciplinary Science
Vol. 2 No. 2 (2023): Maret-April

16S Metagenomics Of Branch-Leaf Compost At The Expo Memorial Park Osaka: 16S Metagenomik Kompos Cabang-Daun Di The Expo Memorial Park Osaka

Fera Aulia (Universitas Negeri Yogyakarta)
Risma Wiharyanti (Universitas Negeri Yogyakarta)
Karan Lohmaneeratana (Kasetsart University)

Article Info

Publish Date
06 Apr 2023

Abstract

Compost is one of the potential microbial habitats for the discovery of a wide range of novel biocatalysts. Compost sample originally made of plant litter, were collected at the Expo Park (Osaka, Japan). The temperature of composts is around 50 °C. Metagenomic DNA was isolated from compost and was used to amplify partial 16S rRNA genes (rDNAs) by polymerase chain reaction for investigation of the microbial diversity in the compost. Molecular microbial identification was carried out by metagenome analysis of 16S rRNA using MinION Next Generation Sequencing (NGS). The results indicated that branch-leaf compost sample from Expo Park is dominated by thermophilic microorganism. The majority of bacteria are Caldicoprobacter, Hungateiclostridium, and Thermoclostridium. To conclude, thermophilic microorganism is not only found in extreme environment, they also presence even in moderate temperature.

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Journal Info
PREVENIRE: Journal of Multidisciplinary Science
Website

Abbrev

prevenire

Publisher

Institut Studi Islam Sunan Doe

Subject

Religion Arts Humanities Computer Science & IT Economics, Econometrics & Finance Education Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

Description

This journal is dedicated to exploring and disseminating the results of various creative and innovative thoughts based on scientific research and thought processes. This Journal is a double-blind review journal focused on empirical research that provides novelty to the research with the latest ...