Consilium Sanitatis: Journal of Health Science and Policy
Vol. 1 No. 2 (2023): April

Desain Primer dan Amplifikasi Gen PhtD dari Streptococcus pneumoniae secara in silico Sebagai Kandidat Vaksin Pneumonia Independent Serotype Berbasis Protein

Risma Wiharyanti (Universitas Negeri Yogyakarta)
Fera Aulia (Universitas Negeri Yogyakarta)
Astiningdiah Merdekawati (PT. Bio Farma)

Article Info

Publish Date
09 Apr 2023

Abstract

Purpose: The aim of this study was to obtain a primer design capable of amplifying the PhtD gene from various S. pneumoniae bacterial serotypes as a protein-based pneumonia vaccine candidate that does not depend on bacterial serotypes.Methods: Primary preparation was carried out through a bioinformatics approach using several software followed by testing the use of primers in the amplification process which was carried out in silico.Results: The results of this study were a pair of primers capable of amplifying the PhtD gene from several S. pneumoniae serotypes.Conclusion: The PhtD gene of the 6 serotypes of S. pneumoniae has high similarities, making it suitable for use as a protein-based vaccine candidate. Based on the consensus of the six serotypes, a pair of primers was obtained that were able to amplify 17 S. pneumoniae strains from several serotypes contained in the reference sequence in silico.

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Journal Info
Consilium Sanitatis: Journal of Health Science and Policy
Website

Abbrev

jhsp

Publisher

Edupedia Publisher

Subject

Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

Description

Consilium Sanitatis: Journal of Health Science and Policy (JHSP) is a peer-reviewed and open-access journal that Focus on publishing original research articles or review articles in the scope of, but are not limited to, the following fields of: Health in General, Public Health, Clinical Health ...