<![CDATA[AbstractsMagnetite nanoparticles of different size ranging 11.9-14.9 nm have been synthesized chemically by coprecipitation method. It was synthesized by FeSO4.7H2O and FeCl3.6H2O with ratio 1:2. The spinel cubicstructure was obtained and was identified by x-ray and transmission electron microscopy (TEM). The result of magnetic properties measurement using vibrating sample magnetometer (VSM) shows that the smaller grain size of magnetite nanoparticles has higher response to external magnetic field and has low coercivity. The potency analyses of magnetite nanoparticles as active materials on SPR-based biosensorapplication were done by making single layer on surface of magnetite nanoparticles using poly ethylene glycol (PEG-4000). After that, the target biomolecules, alpha-amylase, was added on magnetitenanoparticles which were modified by PEG-4000. According to image which was taken by microscope, surface modification of magnetite nanoparticles can improve their dispersibility in fluid. The analysis ofinfra-red spectroscopy (FTIR) shows that surface of magnetite nanoparticles can be modified with PEG- 4000. The covalent bond Fe-O has been created by Fe atoms of magnetite and O atoms of PEG-4000. Inaddition, FTIR analysis shows that PEG-4000 layer can bind alpha-amylase (biomolecules) effectively. So, the smallet grain size of magnetite nanoparticles has great potency to improve immobilization ofbiomolecules on the sensing surface of SPR-based biosensor.Keywords : magnetite nanoparticles, surface modification, biomolecules.]]>
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