Indonesian Journal of Biotechnology
Vol 15, No 1 (2010)

Purification and Characterization of Streptomyces sp. IK Chitinase

Margino, Sebastian (Unknown)
Nugroho, Agustinus Joko (Unknown)
Asmara, Widya (Unknown)

Article Info

Publish Date
09 Nov 2015

Abstract

Streptomyces sp. IK isolated from compost inoculants, could produce extra cellular chitinase in a medium containing 0.2% (w/v) colloidal chitin, fermented for 96 hours at 30oC. The enzyme was purified by a combination of ammonium sulphate precipitation and DEAE-Cellulose anion-exchange chromatography. On SDS-polyacrylamide gel electrophoresis analysis, the purified enzyme showed a mass of 71 kDa. Chitinase was optimally active at pH of 6.7 and at 37oC. Km value and Vmax of the protein for colloidal chitin were 2.92 mg/ml and 4.26 ìg/h, respectively.Key words : chitinase, Streptomyces, purification, characterization

Copyrights © 2010




Citation Download
RIS
EndNote, Reference Manager, ProCite
BibTex
Latex, Jabref
Full PDF
241.573 KB
Original Source
Download Original
Google Scholar
Check in Google Scholar
Journal Info
Indonesian Journal of Biotechnology
Website

Abbrev

ijbiotech

Publisher

Universitas Gadjah Mada

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

Description

The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...