Indonesian Journal of Science and Technology
Vol 1, No 1 (2016): IJoST: VOLUME 1, ISSUE 1, April 2016

A novel In-situ Enzymatic Cleaning Method for Reducing Membrane Fouling in Membrane Bioreactors (MBRs)

M. R. Bilad (Centre for Surface Chemistry and Catalysis, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, Box 2461, 3001 Leuven, Belgium. Singapore Membrane Technology Centre, Nanyang Environment and Water Research Insti)
M. Baten (Centre for Surface Chemistry and Catalysis, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, Box 2461, 3001 Leuven, Belgium)
A. Pollet (Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20 – bus 2463, B-3001 Leuven, Belgium)
C. Courtin (Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20 – bus 2463, B-3001 Leuven, Belgium)
J. Wouters (Department of Chemistry, K.U. Leuven and Institute of Nanoscale Physics and Chemistry (INPAC), Celestijnenlaan 200D, 3001 Heverlee, Belgium)
T. Verbiest (Department of Chemistry, K.U. Leuven and Institute of Nanoscale Physics and Chemistry (INPAC), Celestijnenlaan 200D, 3001 Heverlee, Belgium)
Ivo F J. Vankelecom (Centre for Surface Chemistry and Catalysis, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, Box 2461, 3001 Leuven, Belgium.)



Article Info

Publish Date
01 Apr 2016

Abstract

A novel in-situ enzymatic cleaning method was developed for fouling control in membrane bioreactors (MBRs). It is achieved by bringing the required enzymes near the membrane surface by pulling the enzymes to a magnetic membrane (MM) surface by means of magnetic forces, exactly where the cleaning is required. To achieve this, the enzyme was coupled to a magnetic nanoparticle (MNP) and the membrane it self was loaded with MNP. The magnetic activity was turned by means of an external permanent magnet. The effectiveness of concept was tested in a submerged membrane filtration using the model enzyme-substrate of Bacillus subitilis xylanase-arabinoxylan. The MM had almost similar properties compared to the unloaded ones, except for its well distributed MNPs. The enzyme was stable during coupling conditions and the presence of coupling could be detected using a high-performance anion-exchange chromatography (HPAEC) analysis and Fourier transform infrared spectroscopy (FTIR). The system facilitated an in-situ enzymatic cleaning and could be effectively applied for control fouling in membrane bioreactors (MBRs).

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