Bulletin of Chemical Reaction Engineering & Catalysis
2016: BCREC Volume 11 Issue 3 Year 2016 (December 2016)

Surface Modification of Macroporous Matrix for Immobilization of Lipase for Fructose Oleic Ester Synthesis

Hani Hilmanto (Graduate Program on Estate Crop Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta 55281)
Chusnul Hidayat (Graduate Program on Estate Crop Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta 55281)
Pudji Hastuti (Graduate Program on Estate Crop Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta 55281)



Article Info

Publish Date
11 Oct 2016

Abstract

The objective of this research was to modify the matrix surfaces to obtain both hydrophobic matrix (HM) and hydrophilic-hydrophobic matrix (HHM) for enzymatic synthesis of fructose oleic ester (FOE). The modification was performed by the attachment of 2-phenylpropionaldehyde (PPA) and PPA followed by polyethyleneimine (PEI) for HM and HHM, respectively. The results from FT-IR analysis showed that the peak of stretching vibration of NH2 bond decreased and it was followed by an increase of the peak vibration of –C=N– bond at wave number 1667 cm-1. The peak of bending vibrations of the C=C bond also increased. It indicated that PPA was successfully attached on matrix. For HHM, an increase in the peak area of NH2 bond indicated that PEI was also successfully attached on the matrix. The optimum conditions of lipase adsorption were obtained at buffer pH 7 containing 0.5 M NaCl (9.27 mg protein/g matrix) and without NaCl (9.23 mg protein/g matrix) for HM and HHM, respectively. For FOE synthesis, the best immobilized lipase concentration was about 8% and 6% for HM and HHM, respectively. The optimum time of esterification was 24 h and 18 h for HM and HHM, respectively, in which the yields were 75.96% and 85.29%, respectively. The immobilized lipase could be used up to 3 cycles of esterification reaction. 

Copyrights © 2016






Journal Info

Abbrev

bcrec

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

Bulletin of Chemical Reaction Engineering & Catalysis (e-ISSN: 1978-2993), an international journal, provides a forum for publishing the novel technologies related to the catalyst, catalysis, chemical reactor, kinetics studies, and chemical reaction ...