Journal of Agromedicine and Medical Sciences
Vol 3 No 1 (2017)

The Effect of Hyaluronic Acid on MMP-9 Expression in Trabecular Meshwork Cell Culture of Patient with Primary Open Angle Glaucoma

Cicih Komariah (Unknown)
Ma’sum Effendi (Unknown)
Hidayat Sujuti (Unknown)



Article Info

Publish Date
12 Feb 2017

Abstract

In primary open angle glaucoma, decrease of hyaluronic acid level is related to extracellular matrix deposition of trabecular meshwork. Extracellular matrix deposition will increase humor aqueous outflow resistance which in turn increase the intraocular pressure. MMP-9 as one of matrix metalloproteinase (MMP) family, has a high potency to degrade the extracellular matrix. The expression of MMP-9 can be activated by hyaluronic acid through its binding with CD44 as hyaluronic main receptor. The aim of this research is  to determine the effect of hyaluronic acid on MMP-9 expression in trabecular meshwork cell culture of patient with primary open angle glaucoma by immunocytochemical method. Trabecular meshwork cell culture were exposed to hyaluronic acid at four different concentration (0mg/ml, 1 mg/mL, 3 mg/mL, 6 mg/mL). After 24 hours incubated, we find a significant difference of MMP-9 expression between control group and group with hyaluronic acid. There was significant correlation between hyaluronic acid and MMP-9 expression. Hyaluronic acid exposure will increase MMP-9 expression in trabecular meshwork cell culture. This finding suggest that hyaluronic acid can influence the resistance of humor aqueous outflow in trabecular meshwork by up-regulating MMP-9 expression, and therefore will increase extracellular matrix degradation.Key words : hyaluronic acid, primary open angle glaucoma, MMP-9

Copyrights © 2017






Journal Info

Abbrev

JAMS

Publisher

Subject

Health Professions

Description

Jurnal Agromedicine and Medical Sciences (AMS) adalah jurnal berkala empat bulan (Februari, Juni dan Oktober) yang berisi berbagai artikel dalam bentuk penelitian, tinjauan sistematis dan laporan kasus dalam bidang kedokteran dengan fokus pada ilmu-ilmu kedokteran dasar, kedokteran klinis dan ...