BERITA BIOLOGI
Vol 12, No 3 (2013)

SKRINING DAN ISOLASI METABOLIT AKTIF ANTIBAKTERI KULTUR JAMUR ENDOFIT DARI TUMBUHAN Albertisia papuana Becc. [Screening and Isolation of Antibacterial Active Metabolite from the Culture of Endophytic Fungi from Albertisia papuana Becc.]

Fathoni, Ahmad (Unknown)
Ilyas, Muhammad (Unknown)
Cahyana, Antonius Herry (Unknown)
Agusta, Andria (Unknown)

Article Info

Publish Date
06 Jul 2015

Abstract

Totally 15 isolates of endophytic fungi were obtained from leaves and young stems of a medicinal plant Albertisia papuana Becc.(Menispermaceae). The antibacterial screening of the ethyl acetate extract derived from the fungi cultures in potato dextrose broth (PDB)were performed on a non eluted Thin Layer Chromatography Bioautography assay (TLC bioautography assay). From the screening test, itwas found that the ethyl acetate extract of the fungus Xylaria sp. DAP-KRI-5 culture showed strong antibacterial activity against Staphylococcus aureus. The isolation of active metabolite based on the bioautography guided assay from ethyl acetate extract of the Xylaria sp. DAP-KRI-5, led us to identify phloroglucinol as a main antibacterial compound. The chemical structure of phloroglucinol was deduced from it spectral data, including UV-Vis, 1H and 13C-NMR, GC-MS and published data elsewhere.

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Journal Info
BERITA BIOLOGI
Website

Abbrev

berita_biologi

Publisher

Lembaga Ilmu Pengetahuan Indonesia

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

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