JURNAL BIOLOGI INDONESIA
Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA

Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Antibody in Chickens Using Local Isolate of PTS III

Indriani, Risa (Unknown)
Indi Dharmayanti, NLP. (Unknown)

Article Info

Publish Date
27 Dec 2013

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectiousbronchitis (IBV). Antigen was prepared from whole virus of infectious bronchitis local isolate PTS-III serotype.Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera indeveloped ELISA were determined 0.4μg/well, 1:2000 and 1:100, respectively. Correlation optical densities (OD)were compared with a standard commercial ELISA (R2=0.933). The developed ELISA has a better sensitivity to hemagglutinationinhibition (HI) test. The developed local isolate ELISA can be used to detect antibody against infectiousbronchitis virus and it is suitable for sample screening at the diagnostic laboratories.Keywords: ELISA, antibody, chicken, IB PTS-III local isolate

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Journal Info
JURNAL BIOLOGI INDONESIA
Website

Abbrev

jurnal_biologi_indonesia

Publisher

Lembaga Ilmu Pengetahuan Indonesia

Subject

Biochemistry, Genetics & Molecular Biology

Description

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