The condition of starter plays important role in enzyme production. Optimum starter will lead to the optimum yield of enzyme production. The experiments which were aimed to obtain the optimum age for starter of Streptomyces thioluteus TTA 02 SDS 14 from solid as well as liquid medium had been carried out which would be used for transglutaminase production. Medium used for culture maintenance, starter optimization and enzyme production was described by Bahrim. The result showed that S. thioluteus TTA 02 SDS 14 was ready to be used as starter after being cultured on solid medium continued in liquid medium each for 6 days. The enzyme production in a bioreactor using optimized starter produced enzyme with the highest activity after being fermented for 2 days with 150 rpm agitation. The crude enzyme active optimally at 45-50 oC, pH of 6 and no effect of metal ion and inhibitor on enzyme activity.
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