Jurnal Kimia Terapan Indonesia
Vol 4, No 1 (1994)

PEMURNIAN GLUKOAMILASE DARI HASIL FERMENTASI KAPANG RHIZOPUS ORYZAE

Linar Z. Udin (Unknown)
Rini Noviyanti (Unknown)
A. Sidik (Unknown)
A. T. Karossi (Unknown)

Article Info

Publish Date
02 Mar 2017

Abstract

Purification of the glucoamylase R. oryzae was carried out by addition of ammonium sulfate 80% saturation, on the fermentation broth at 4°C. The precipitate formed by centrifugation at 9000 rpm was then dialyzed in buffer solution and then concentrated using freeze dryer. It was found that the specific activity of the enzyme was around three-fold higher the crudeenzyme from fermentation broth and the purity of the enzyme was almost twelve-fold.purer than the crude enzyme. The molecular weight of the glucoamylase was found to be 36,000 as determined by SDS gel electrophoresis. The optimum pH witli soluble starch as substrate was at pH 4.5 and the optimum temperature was 55°C while the Km Value was 0.027%.

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Journal Info
Jurnal Kimia Terapan Indonesia
Website

Abbrev

InaJAC

Publisher

Lembaga Ilmu Pengetahuan Indonesia

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

Description

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