ANNALES BOGORIENSES
Vol 11, No 1 (2007): Annales Bogorienses

Motif Ii of Japanese Encephalitis Vius Ns3 Pro is Not Essential for Rna Binding Activit

Utama, Andi (Unknown)
Shimizu, Hiroyuki (Unknown)

Article Info

Publish Date
08 Jun 2007

Abstract

The role of motif n  (DEAR AsPw-GlulK,,-Ala.,.7-Hi  1~~  )  of Japanese encephalitis  virus (JEV) NS3 protein on RNA binding activity was studied. A point mutation was introduced to  the motif and  the RNA binding activity of each mutant protein was analyzed. Truncated form of each protein with a His-tag was expressed m Escherichia coli BL2l(DE3) pLysS and purified by metal affinity resin. Asp-285 - and Glu-286 was respectively substituted with Ala, AJa-287 was replaced by Cys.,Gly.  or ser. His-288 was mutated to other 19 amino acids. In total. 24 mutant proteins were produced and analyzed. AL results, all mutants showed quite similar RNA binding activity.  Indicating that motif II of JEY NS3 is not related to RNA binding activity. The same finding was reported for hepatitis C virus c (Hey) NS3 protein, suggesting the similar structure of NS3 protein in flavivirus .Keywords: Japanese encephalitis, R.binding, motif

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Journal Info
ANNALES BOGORIENSES
Website

Abbrev

annales

Publisher

Lembaga Ilmu Pengetahuan Indonesia

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

Description

The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as ...