Biosaintifika: Journal of Biology & Biology Education
Vol 9, No 3 (2017): December 2017

Expression of Mycobacterium tuberculosis Protein Tyrosine Phosphatase B in Escherichia coli and Its Recovery from Inclusion Body

Savalas, Lalu Rudyat Telly (Unknown)
Sedijani, Prapti (Unknown)
Hadisaputra, Saprizal (Unknown)
Ardhuha, Jannatin (Unknown)
Lestari, Chomsa Asih (Unknown)
Wahidah, Etty Nurul (Unknown)

Article Info

Publish Date
31 Dec 2017

Abstract

The present study aims at expressing and partially purifying PtpB in active form. To achieve this, Mtb PtpB gene has been cloned into pET30a vector and overexpressed in Escherichia coli BL 21(DE3) under IPTG induction in the form of an inclusion body. Following resolubilization by urea and dialysis, the resulted PtpB has been shown to be active against para-Nitrophenyl phosphate. It is concluded that the resulted PtpB has had been recovered from inclusion body to give the active form of the enzyme, and thus the success in overexpressing PtpB provides the required material to investigate the biochemical properties of the pathogen virulence factor further.

Copyrights © 2017




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Journal Info
Biosaintifika: Journal of Biology & Biology Education
Website

Abbrev

biosaintifika

Publisher

Universitas Negeri Semarang

Subject

Agriculture, Biological Sciences & Forestry

Description

Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological ...