Journal of Tropical Pharmacy and Chemistry
Vol. 1 No. 4 (2012): Journal of Tropical Pharmacy and Chemistry

Analisis Fitokimia Senyawa Metabolit Sekunder Ekstrak Kasar Etanol Daun Meranti Merah (Shorea leprosula Miq.) dan Sifat Antibakterinya terhadap Staphylococcus aureus dan Eschericia coli

Sudrajat Sudrajat (Prodi Biologi, FMIPA, Universitas Mulawarman, Samarinda, Indonesia)
Sadani Sadani (Prodi Biologi, FMIPA, Universitas Mulawarman, Samarinda, Indonesia)
Sudiastuti Sudiastuti (Prodi Biologi, FMIPA, Universitas Mulawarman, Samarinda, Indonesia)



Article Info

Publish Date
30 Jun 2012

Abstract

This study aims to determine the content of secondary metabolites and antibacterial power crude extract copper leaf meranti (Shorea leprosula Miq.) against Staphylococcus aureus and Escherichia coli. Antibacterial power is determined by the size of inhibition zone formed using paper disc diffusion method of Kirby-Bauer. The research design using a completely randomized design (CRD), with a concentration of 6 treatments and 4 replication for each type of bacteria. Variations in the concentration of a given treatment consisting of 0%, 3.75%, 7.5%, 11.25%, 15%, and chloramphenicol (positive control). The results shown that crude extract copper leaf meranti (Shorea leprosula Miq.) contain secondary metabolites such as alkaloids, saponins, triterpenoids, flavonoids, phenols and antibacterial power against Staphylococcus aureus and Escherichia coli, with the relatively strong antibacterial category. Anova analysis showed that the value of F count = 41,190 with sig = 0.000 <0.05, which means that H0 is rejected, in other words, treatment factors meranti copper leaf crude extract with various concentrations of 3.75%, 7.5%, 11, 25%, and 15%. different significantly affect the bacterial inhibition zone for S. aureus. The result same effect shown onE. coli demonstrated the value Fcount = 81, 236 with sig = 0.000 <0.05. These results demonstrate the influence of crude extract of leaf meranticopper very significantly to the growth of bacteria S. aureus and E. coli. At S.aureus, treatment concentration of 3.75%, 7.5%, 11.25%, and 15%yielding different significant of inhibition zone. In the E.colibacterial, treatment concentration of 3.75%, 7.5%, 11.25%, and 15% yielding different significant of inhibition zone.Best concentration as antibacterial S.aureus and E.coli have been shown on providing the lowest treatment concentration (3.75%), which shows the power antibacterial properties not significantly different from the concentration on it. Keywords: Leaf of Shorea leprosula Miq, secondary metabolite substance, antibacterial, Staphylococcusaureus, Escherichia coli Abstrak Penelitian ini bertujuan untuk mengetahui kandungan senyawa metabolit sekunder dan daya antibakteri ekstrak kasar daun meranti tembaga (Shorea leprosula Miq.) terhadap bakteri Staphylococcus aureus danEscherichia coli.Ukuran daya antibakteri ditentukan oleh zona hambat yang terbentuk menggunakan metode difusi kertas cakram Kirby-Bauer.Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL), dengan konsentrasi dari 6 perlakuan dan 4 replikasi untuk setiap jenis bakteri.Variasi konsentrasi perlakuan yang diberikan terdiri atas 0%, 3.75%, 7.5%, 11.25%, 15% dan kloramfenikol (kontrol positif). Hasil penelitian menunujukkan bahwa ekstrak kasar daun meranti tembaga (Shorea leprosula Miq.) mengandung senyawa metabolit sekunder seperti alkaloid, saponin, triterpenoid, flavonoid, fenol dan daya antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli, dengan kategori antibakteri tergolong kuat.Analisis anava menunjukkan bahwa nilai F hitung = 41.190 dengannilai sig = 0,000 < 0,05, yang berarti bahwa H0ditolak, dengan kata lain faktor perlakuan ekstrak kasar daun meranti tembaga dengan variasi konsentrasi 3,75%, 7,5%, 11,25%, dan 15 %. berpengaruhberbeda secara sangat signifikan terhadap zona hambat untuk bakteri S. aureus.Hal yang sama ditunjukkan pengaruhnya terhadap E.coli dengan nilai Fhitung = 81.236 dengannilai sig = 0,000 < 0,05. Hasil ini menunjukkan adanya pengaruh ekstrak kasar daun meranti termbaga secara sangatsignifikan terhadap pertumbuhan bakteri S. aureus dan E. coli. Pada bakteri Staphylococcus aureus,perlakuan konsentrasi 3.75%, 7.5%, 11.25%, dan 15% menghasilkan zona hambat yangberbeda signifikan. Pada bakteri Escherichia coli, perlakuan konsentrasi 3.75%, 7,5%, 11.25%, dan 15% menghasilkan zona hambat yang berbeda signifikan. Konsentrasi terbaik sebagai antibakteri S.aureus dan E.coli telah ditunjukkan pada pemberian konsentrasi perlakuan terendah (3,75 %), yang menunjukkan sifat daya antibakterinya tidak berbeda nyata dengan konsentrasi di atasnya. Kata Kunci : Daun meranti tembaga (Shorea leprosula Miq.), zat bioaktif, antibakteri, Staphylococcus aureus, Escherichia coli

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Journal Info

Abbrev

jtpc

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Medicine & Pharmacology Public Health

Description

Journal of Tropical Pharmacy and Chemistry is a Biannual, international, open access (Indexed in DOAJ, Crossref, SINTA, PKP Index, BASE, Moraref, Google Scholar), journal dedicated to various disciplines of pharmaceutical and allied sciences. Journal of Tropical Pharmacy and Chemistry (ISSN-p: ...