Journal of Tropical Pharmacy and Chemistry
Vol. 4 No. 1 (2017): Journal of Tropical Pharmacy and Chemistry

The Immunosuppressant Effect Comparation Between Ethyl Acetate and n-Butanol Fractions of Kalanchoe Pinnata (Lmk) Pers In 2,6,10,14 Tetramethylpentadecane-Treated Mice

Niken Indriyanti (Department of Pharmacology, Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia)
Joewono Soeroso (Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia)
Junaidi Khotib (Department of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia)



Article Info

Publish Date
30 Jun 2017

Abstract

Immunosuppressant drugs are the main treatment of lupus patient. The ACR and SLICC treatment guidelines are able to increase the quality of life, but the outcome is not satisfying since the off-label therapy of corticosteroids and cytotoxic drugs give a lot of side effects. Many breakthrough efforts still develop in order to find the safe and effective drugs for lupus, such as finding immunosuppressant drugs from natural resources. One of the potential resources is Kalanchoe pinnata (Lmk) Pers, which have immunosuppressant, anti-inflammatory, antinociceptive, and antioxidant effects. Thus, in the previous study, we found the effect of the aqueous extract of Kalanchoe pinnata (Lmk) Pers is active to repair the lupus manifestation in 2,6,10,14 tetramethylpentadecane (TMPD)-treated mice. Then, this research was focused on the in vivo immunosuppressant effect of a flavonoid-rich fraction of the extract which was consisted of the ethyl acetate (FE) and n-butanol (FB) fractions. The induction method and the extraction procedure were the same as the previous study and then the fractionation was performed by using liquid-liquid extraction. After 2-week treatment of both fractions, we obtained the differences in the total leukocytes, organ indexes, and also the spleen, kidney, and joint structure parameters. The total leukocyte of the FE group was 3,600±264 cells/mm3, which was lower than that in the FB group. The spleen and kidney indexes increased after the administration of FB fraction, while the FE fraction was not. At last, despite the histology observation of spleen resembled mild structural changes differences, the clear differences between both treatment groups occurred in the kidney and joint histology. The differences led to a conclusion that the FE fraction has the better immunosuppressant effect in TMPD-treated mice.

Copyrights © 2017






Journal Info

Abbrev

jtpc

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Medicine & Pharmacology Public Health

Description

Journal of Tropical Pharmacy and Chemistry is a Biannual, international, open access (Indexed in DOAJ, Crossref, SINTA, PKP Index, BASE, Moraref, Google Scholar), journal dedicated to various disciplines of pharmaceutical and allied sciences. Journal of Tropical Pharmacy and Chemistry (ISSN-p: ...