Teknologi Indonesia
Vol 36, No 3 (2013)

PECTINASE PRODUCTION BY ASPERGILLUS USTUS BL5 AT SOLID STATE FERMENTATION MEDIUM USING AGRICULTURAL BIOMASS

Rahmani, Nanik (Unknown)
Andriani, Ade (Unknown)
Anggraini, Yufi Sara (Unknown)
Yopi, - (Unknown)



Article Info

Publish Date
24 Feb 2015

Abstract

This research showed that Indonesian agricultural biomasses, such as Siam orange peel, Medan orange peel, Durian peel and old tea leaves contain a pectin that are a potential material for use as a substrate for pectinase production. From the four types of biomasses used, Medan orange peel produced pectinase activity with the highest value equal to 1.28 U/mL. Optimization of fermentation conditions showed that Aspergillus ustus BL5 pectinase production were affected by the concentration of substrate, media pH, and temperature of the fermentation. Optimization of process showed that the optimum substrate concentration, pH and temperatuter were 10%, 4 and 40C with pectinase activity of 1.37 U/mL, 2.85 U/mL, and 1.92 U/mL respectively. Pectin content in the medium has a proportional relationship with the activity of the enzyme pectinase produced. The high content of pectin in Medanorange peel making pectinase enzyme activity produced on the substrate is also high.

Copyrights © 2013






Journal Info

Abbrev

JTI

Publisher

Subject

Computer Science & IT

Description

JTI is a journal in the Departement of Engineering Sciences - Indonesian Institute of Sciences (LIPI). JTI has policy to publish a new and original research paper or a review paper in The scope of Technology. JTI publishes two issues per year. The journal has been registered with printed-ISSN ...