<![CDATA[ABSTRAK Latar belakang Berbagai faktor dapat mempengaruhi prognosis penderita karsinoma payudara, salah satunya adalah invasi limfatik. Identifikasi invasi limfatik pada preparat hematoksilin eosin (HE) sulit sehingga diperlukan metode alternatif yaitu dengan pulasan imunohistokimia Vascular Endothelial Growth Factor Receptor-3 (VEGFR-3). Penelitian menggunakan pulasan VEGFR-3 dalam mendiagnosis invasi limfatik pada karsinoma payudara belum pernah dilakukan. Penelitian ini bertujuan untuk menganalisa tingkat kesesuaian diagnosis invasi limfatik pada karsinoma duktal invasif payudara antara pulasan VEGFR-3 dan pulasan HE. Metode Lima puluh satu blok parafin penderita karsinoma duktal invasif payudara tipe tidak spesifik dilakukan pemotongan ulang dan dipulas dengan HE dan VEGFR-3. Semua sediaan dievaluasi oleh dua kelompok pengamat untuk didiagnosis invasi limfatik peritumoral. Kemudian dihitung persentase kesesuaian dan nilai kappa. Hasil Pada pulasan HE didapatkan kesesuaian diagnosis invasi limfatik sebesar 78,4%, dengan nilai κ=0,45; SE=0,14; 95%CI=0,31-0,59. Sedang pada pulasan imunohistokimia dengan VEGFR-3 didapatkan kesesuaian diagnosis invasi limfatik sebesar 92,1%, dengan nilai κ=0,73; SE=0,13; 95%CI=0,60-0,86. Ekspresi VEGFR-3 dapat ditemukan pada sebagian besar kasus. Kesimpulan Pada karsinoma duktal invasif tipe tidak spesifik, VEGFR-3 dapat digunakan untuk menilai tingkat kesesuaian invasi limfatik peritumoral, dan untuk memulas sitoplasma sel tumor. Kata kunci: invasi limfatik, VEGFR-3, HE, kesesuaian, karsinoma payudara ABSTRACT Background There are many factors influence the prognosis of breast cancer patients, one of them is lymphatic invasion. Identification of lymphatic invasion on hematoxyllin eosin (HE) slide is very difficult, so an alternative methode is needed, that is using anti VEGFR-3 antibody. Research of VEGFR-3 in diagnosing lymphatic invasion of carcinoma mammae has not doneyet. The aim of the study is to analysis the level of diagnosis invasive ductal lymphatic carcinoma ductal mammae between VEGFR-3 and HE. Methods Fifty one paraffin block patient of invasive ductal carcinoma mammae NOS were process and stained using HE and VEGFR-3. All slides were analyzed by two groups observers for peritumoral lymphatic invasion. Scoring and kappa were done. Results Observed agreement of HE staining was 78,4%, with κ=0,45; SE=0,14; 95%CI=0,31-0,59. While, observed level agreement of VEGFR-3 immunostaining was 92,1%, with κ=0,73; SE=0,13; 95%CI=0,60-0,86. VEGFR-3 expression could be found on almost cases that the cytoplasm of cancer tumor cells were showing positive staining for in most of the cases. Conclussion On invasive ductal carcinoma NOS, VEGFR-3 could be used to observed level agreement of lymphatic invasion peritumoral, and could staining the cytoplasm of cancer cells. Keyword: lymphatic invasion, VEGFR-3, HE, agreement, breast cancer]]>
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