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Jurnal Teknologi Laboratorium
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Articles 97 Documents
Identifikasi Candida Albican Pada Saliva Wanita Penderita Diabetes Melitus Farizal, Jon; Abdul Rahman Serbasa Dewa, Exchagusesa
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.538 KB) | DOI: 10.29238/teknolabjournal.v6i2.44

Abstract

Diabetes mellitus is a predisposing factor for the onset of oral candidiasis. Women with diabetes have extra sugar in the secretion of saliva. Sugar contained in saliva are stacked on the mucosa so provide food for mold growth so that the diabetic patient saliva will be found Candida albicans The aim of this study was to identify Candida albicans in saliva of women with diabetes mellitus in patient wards of Medicine Hospital Dr M.Yunus Bengkulu 2017.This type of research is conducted by using descriptive survey design. Sampling technique using Purposive Sampling, the number of samples identified as many as 27, conducted by macroscopic and microscopic examination. Data in this research using descriptive analysis.Results of univariate analysis obtained, all respondents the majority of respondents 52% positive saliva fungus Candida albicans and almost half of respondents 48% negative saliva fungus Candida albicans. The prevalence values indicate that the majority of the total population of women with DM saliva samples contained fungus the Candida albicans. Whole saliva respondents (100%) in women with diabetes is growing colonies on Media SDA (Saboraud Dextrose Agar) fungus Candida albicans
Efektivitas Berbagai Konsentrasi Infusa Daun Salam (Eugenia polyantha Wight) terhadap Daya Antibakteri Staphylococcus aureus Secara In Vitro Apriani, Dewi; Amaliawati, Nur; Kurniati, Eni
Jurnal Teknologi Laboratorium Vol 3 No 1 (2014): 2014 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Staphylococcus aureus is one of the acne-causing bacterias. Chemical compound in the Bay leaves are well-known as an antibacterial agent to inhibit the growth of Staphylococcus aureus. This study aimed to determine the influence of various Bay leaf (Eugenia polyantha Wight) infusing concentration toward the inhibition of Staphylococcus aureus growth. This research is experimental study with post-test and control group design. The sensitivity test methods toward Staphylococcus aureus were divided into five groups of bay leaf infusing concentration of 60%, 70%, 80%, 90%, and 100%. This method are used in 6 times to obtained 30 datas. Then, the datas were analyzed descriptively and analytically by ANOVA using SPSS 16.0 for Windows. The infusion of Bay leaves had inhibition activity against Staphylococcus aureus growth based on ANOVA test which showed a significance value 0.000 <0.05. There was a proof that the infusion of Bay leave have an effect to inhibit the growth of Staphylococcus aureus.
Kadar Protein Total dan Ureum Dengan dan Tanpa Penambahan γ-cyclodextrin Pada Serum Lipemik Sujono, Sujono; Maulida, Yumna Ayu; Sari, Meilanda Puspita
Jurnal Teknologi Laboratorium Vol 5 No 1 (2016): 2016 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Lipemic serum is a turbidity serum caused by accumulation of lipoprotein particle. This case distrupt any kinds of examination at clinic laboratory which one of them is examining Total Protein. It can be carried out by adding γ-cyclodextrin in order to handle the turbidity on lipemic serum. This study aims at finding the Total Protein’s levels overview on lipemic serum with and without addition of γ-cyclodextrin. This study was pre-eksperimental design byusing Post Test Only with Control Group Design. This study was done at Chemistry Laboratory, Health Analysis Study Program in April 2016. The sample of the data was 20 lipemic serum. In examining of total proteis’s level used Biuret method and ureum level used Barthelot method from lipemic serum with and without addition ofγ-cyclodextrin 20%.There is a significant difference between total protein and ureum, the difference average of total protein level and ureum level before and after adding γ-cyclodextrin was 1,311 g/dL (12,40%) and 6.38 mg/dL (10.88%).
Gambaran Sedimen Urine Pada Masyarakat Yang Mengkonsumsi Air Pegunungan Di Kecamatan Kendari Barat Kota Kendari Mongan, Ruth; Supiati, Supiati; Mangiri, Susi
Jurnal Teknologi Laboratorium Vol 6 No 1 (2017): 2017 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.157 KB) | DOI: 10.29238/teknolabjournal.v6i1.88

Abstract

Air yang bersih dan sehat merupakan kualifikasi yang sangat diperlukan untuk pemenuhan kebutuhan manusia. Air harus mempunyai persyaratan khusus agar air tersebut tidak menimbulkan penyakit bagi manusia. Air pegunungan yang dikonsumsi masyarakat Kelurahan Sodoha Kota Kendari, pada umumnya memiliki kualitas yang baik, tetapi dapat berubah kualitasnya karena berbagai faktor. Penelitian ini bertujuan untuk mengetahui gambaran sedimen urin pada masyarakat yang mengkonsumsi air pegunungan di Kecamatan Kendari Barat Kota Kendari.Jenis penelitian adalah deskriptif, dengan menentukan prosentase sedimen urin masyarakat yang mengkonsumsi air pegunungan melalui pemeriksaan laboratorium. Jumlah sampel dalam penelitian ini adalah 30 Kepala Keluarga yang tinggal di Kelurahan Sodohoa Kecamatan Kendari Barat Kota Kendari, dengan teknik pengambilan simple random sampling (sampel acak secara sederhana). Berdasarkan hasil penelitian, ditemukan prosentase sedimen urin abnormal, yaitu leuokosit dan eritrosit 6,7 %, epitel 23,3 %, silinder, kalsium oksalat, asam urat, dan bakteri masing-masing 3, 3 %. Kesimpulan dari penelitian menunjukkan adanya sedimen organik yang meliputi, leukosit, eritrosit, silinder, epitel dan bakteri pada masyarakat yang mengkonsumsi air pegunungan Kelurahan Sodohoa Kecamatan Kendari Barat Kota Kendari.
Efektifitas Pemberian Perasan Bawang Putih (Allium Sativum Linn) Dosis Tunggal Terhadap Jumlah Telur Cacing Gelang (Toxocara Canis) Secara In Vivo Utami, Ratri Risky; Nuryati, Anik; Nuryani, Siti
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Background this research is worm disease problematic in the field of health. Treatment for the disease worms that have been used are synthetic drugs that have side effects are not good for health and can lead to dependence. One of the medicinal plants used for deworming is garlic. Chemical constituents in garlic is allicin that is anthelmintics.To prove that it is carried out in vivo study using dogs infected with Toxocara canis worms as test animals.Objective this research to knowing of the effectiveness of various doses of garlic juice with a gift on the first day against Toxocara canis worm egg counts in vivo and the largest decrease in the effectiveness of Toxocara canis worm egg counts after administration of juice garlic at a dose of 0,5; 1 and 1,5 percent weight within 6 days of observation.Methods this research is an experimental study using dogs infected with Toxocara canis nearly the same number of eggs as well as the research design pretest-posttest with control group.Results the largest decrease in the effectiveness of Toxocara canis worm egg counts after administration juice garlic at a dose of 0,5; 1 and 1,5 percent weight within 6 days of observation known to occur on day 3 with a percentage of effectiveness are 48,49%; 67,18%; dan 82,85%. The higher doses of garlic juice, the more effective to decline in the amount of worm eggs Toxocara canis in vivo.Conclusion this research is garlic juice with various doses are not as effective as once treatment in reducing the the amount of eggs bracelet worm (Toxocara canis) in vivo.
Gambaran Kadar Kolesterol-LDL (Low Density Lipoprotein) Sebelum dan 48 Jam Sesudah Melakukan Satu Kali Terapi Bekam Basah Pada Penderita Hipertensi Dengan Pola lima titik Suryanta, Suryanta; Septiana, Winda
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Hypertension, or more commonly known as high blood pressure is a condition in which a person got an increasing blood pressure upper normal, resulting in increasing morbidity and mortality. The long hypertension is one risk factor for cardiovascular disease, which is one cause of atherosclerosis. Atherosclerosis is a very progressive diseases that causes hardening of the arteries due to the blockage by oxidized cholesterol. Atherosclerosis begins with the build up of LDL-cholesterol. There are two handling of LDL-cholesterol; pharmacological and non-pharmacological. Nonpharmacologic is done with wet cupping therapy. The aim of this study is to determine the average LDL-cholesterol levels before and after the wet cupping therapy with five-point pattern. This research is descriptive research, then presented in the form of tables to showing the results of the study. This study was done Talunombo, Sidomulya, Pengasih, Kulon Progo. This research object is venous blood samples taken from hypertensive patients as research subjects. Descriptive test results obtained an average LDL-cholesterol levels before the wet cupping therapy is 114,182 mg/dl and after wet cupping is 115,618 mg/dl. The conclusion of this study is the average LDL-cholesterol levels prior to the wet cupping therapy with a five-point pattern is 114,182 mg/dl and after wet cupping with five-point pattern is 115,618 mg/dl.
Efektivitas Berbagai Konsentrasi Infusa Daun Salam (Eugenia polyantha Wight) terhadap Daya Antibakteri Staphylococcus aureus Secara In Vitro Apriani, Dewi; Amaliwati, Nur; Kurniati, Eni
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.485 KB)

Abstract

Staphylococcus aureus is one of the acne-causing bacterias. Chemical compound in the Bay leaves are well-known as an antibacterial agent to inhibit the growth of Staphylococcus aureus. This study aimed to determine the influence of various Bay leaf (Eugenia polyantha Wight) infusing concentration toward the inhibition of Staphylococcus aureus growth. This research is experimental study with post-test and control group design. The sensitivity test methods toward Staphylococcus aureus were divided into five groups of bay leaf infusing concentration of 60%, 70%, 80%, 90%, and 100%. This method are used in 6 times to obtained 30 datas. Then, the datas were analyzed descriptively and analytically by ANOVA using SPSS 16.0 for Windows. The infusion of Bay leaves had inhibition activity against Staphylococcus aureus growth based on ANOVA test which showed a significance value 0.000 <0.05. There was a proof that the infusion of Bay leave have an effect to inhibit the growth of Staphylococcus aureus.
Gambaran Kadar Glukosa Darah Metode GOD-PAP (Glucose Oxsidase – Peroxidase Aminoantypirin) Sampel Serum dan Plasma EDTA (Ethylen Diamin Terta Acetat) Subiyono, Subiyono; Martsiningsih, M Atik; Gabrela, Denni
Jurnal Teknologi Laboratorium Vol 5 No 1 (2016): 2016 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.451 KB)

Abstract

Pemeriksaan laboratorium sangat diperlukan untuk membantu menegakkan diagnose suatu penyakit dan memperoleh hasil pemeriksaan yang akurat. Pemeriksaan kadar glukosa darah dapat membantu perubahan pola dan gaya hidup sehat, untuk menghindari makanan yang mengandung glukosa tinggi berisiko meningkatkan kadar glukosa darah. Pemeriksaan kadar glukosa biasanya menggunakan sampel serum dan sampel plasma. mengetahui hasil pemeriksaan kadar glukosa darah dan gambaran perbedaan hasil kadar glukosa darah metode GOD-PAP dari sampel serum dan plasma EDTA. Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian deskriptif dalam bentuk table untuk mengetahui selisih rata-rata kadar glukosa darah sampel serum dan plasma EDTA dan grafik untuk mengetahui yang lebih tinggi. Hasil pemeriksaan kadar glukosa darah dengan sampel serum rata – rata 103,7 mg/dl, sedangkan pemeriksaan kadar glukosa darah dengan sampel plasma rata –rata 101,3 mg/dl. Berdasarkan analisa uji deskriptif dinyatakan ada perbedaan hasil pemeriksaan kadar glukosa darah antara sampel serum dan plasma EDTA. Ada perbedaan hasil pemriksaan kadar glukosa darah metode GOD-PAP dengan menggunakan sampel serum dan plasma EDTA.
Pengembangan Mikroskop Dengan Mikrokontroler dan Cahaya Monokromatik Untuk Mendeteksi Parasit Malaria Susanti, Ida; Handayani, Sarwo; Ekowatiningsih, Riyanti; Prasetyorini, Budi; A Yusnita, Endah; Ardianto, Donni Agus; K Widjaya, Sastra
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (683.906 KB) | DOI: 10.29238/teknolabjournal.v6i2.59

Abstract

Malaria still become one of major health burden in Indonesia especially in remote areas of east Indonesia. Golden standard of malaria parasite detection is still microscopic technique using polychromatic light source whether from halogen or natural light source. A microscopic technique has a lot of benefits but still have weaknesses, such as time-consuming and bias on the reading by microscopist, because of artifact in the image. Aims of this study were to designed malaria parasites detection tool that is robust, fast, convenient and clear by minimizing artifact on the slide. Design of this study was laboratory experimental which modified simple microscope into an automatic microscope with table movement and webcam recording using a microcontroller and monochromatic light source. The wavelength of the light sources was 402nm(blue), 532 nm (green) and 650 nm (red), the intensity of each source differed. The reading of the slide image was conducted by two certified microscopists, who read 60 images of a thick and thin slide with three different live stage of Plasmodium falciparum live, which wearing, trophozoite and schizont. This study showed that modification of microscope was succeeded with automatic movement and webcam recording, process time in one step movement and recording approximately 10 seconds or 17minutes for 100 fields of view as confirmation process. The monochromatic light source has proven to give a clear and contrast field of view when the intensities were higher than 40 mW and the certified microscopist able to identified Plasmodium falciparum parasites. Data analysis of microscopist reading used nonparametric statistic Friedman by SPSS showed that correlation between images using monochromatic and polychromatic lights have meaningless differences in a thick and thin slide. However, hemozoin as a marker of Plasmodium falciparum parasite was less detected by monochromatic light used in this study.
PENGGUNAAN BERBAGAI KONSENTRASI EKSTRAK ETHANOL DAUN RAMBUTAN (Nephelium lappaceum L.) TERHADAP MORTALITAS LARVA Culex quinquefasciatus Lestari, Dwi; Nuryati, Anik; Nuryani, Siti
Jurnal Teknologi Laboratorium Vol 3 No 1 (2014): 2014 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.879 KB)

Abstract

Mosquitoes are insects which are responsible in transmission process of many diseases in humans and animals. One type that is often found is Culex quinquefasciatus. Culex quinquefasciatus mosquitoes serve as vectors of disease filariasis and chikungunya. the mosquito's prevention that uses synthetic insecticide affects the negative side for the environment. Natural insecticide is the safe way that can be used as insecticide.Rambutan’s leaves contain compounds of saponin and tanin, it be used for larvacide’s.This research aims to find out about ethanol extract of rambutan's leaves that can be used for larvacide, the impact, the influence of ethanol extract of rambutan's leaves towards larva mortality and the effective concentrate in the researchThis research is quasi experimental with Post Test Control Group Design. The concentrate extract of rambutan's leaves that is used is 0.025%, 0.05%, 0.1%, 0.2%, 0.4%,and 0.8% with duration 24 hours. Then, it is counted the amount of dead larvae. Then, the result is analyzed statically using Anova One Way and linear regression.The result showed that the percentage of the death of larva Culex quinquefasciatus in concentrate 0.025%, 0.05%, 0.1%,0.2%,0.4%, and 0.8% each of them are 77%,86%,87%,95%,98%,and 100%. The result of Anova One Way showed that the significant value is 0.000 (<0.05) which means that there is an impact of using rambutan's leaves extract towards larva mortality Culex quinquefasciatus.The use of Ethanol extract can cause the mortality of larva Culex quinquefasciatus.

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