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Medical Laboratory Technology Journal

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Published by Poltekkes Kemenkes Banjarmasin

ISSN : - EISSN : - DOI : -

Core Subject : Health, Science,

Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area. Please read these guidelines carefully. Authors who want to submit their manuscript to the editorial office of Medical Laboratory Technology Journal should obey the writing guidelines. If the manuscript submitted is not appropriate with the guidelines or written in a different format, it will BE REJECTED by the editors before further reviewed. The editors will only accept the manuscripts which meet the assigned format.

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Articles 7 Documents

Search results for , issue "Vol 4, No 1 (2018): June" : 7 Documents clear

Potention of Active Charcoal from Musa Paradisiaca and Manihot Utilissima Shell in Degrading River Contamination Nurlailah Nurlailah; Anny Thuraidah; Yayuk Kustiningsih
Medical Laboratory Technology Journal Vol 4, No 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

River water is one of type water surface which is a lot of finding in South Kalimantan. Generally, this water used by the citizen for cooking and bathing. But along with era growth, this river becomes as disposal of various industrial waste. The contamination of heavy metal like Fe, Mn, Pb, Cd, and turbidity enhance progressively and degrade the water quality. Some natural substance which can be used to improve river water quality is active charcoal from Musa paradisiaca and Manihot utilissima shell which is easy to get. Target research is determined to find optimum dose of active charcoal from Musa paradisiaca and Manihot utilissima shell that capable to degrade heavy metal contamination and turbidity in water river. It used experimentally with pretest and post-test with control group design. A result of research showed the optimum dose of active charcoal Musa paradisiaca shell was 15 gram , it could absorb Pb 28,8% and Mn 24,6%, but 10 gram can degrade turbidity until 83,8 %, while the optimum dose of Manihot utilissima shell to 25,4% Pb and degraded 77,5% of turbidity was 15 gram. Statistical test result with Kruskal Wallis got the p-value less than 0,05 it meant there was a difference between the treatment of active charcoal from Musa paradisiaca and Manihot utilissima shell in degrading the contamination. A conclusion is those active charcoal having a potential to enhance the water river quality. Suggested to use another activator to the potential like stirring, time of contact and the different mass.

Lime Soap as Bacteria Reducer Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol 4, No 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Some people in South Kalimantan have used river water to fulfill their daily needs include washing the utensils. Wash the cutlery use river water can cause disease transmission. Squeezed lime has an antibacterial effect in inhibiting bacterial growth. Antibacterial dishwashing soap is effective in reducing pathogenic bacteria. This research is to know the difference of bacterial amount on the variation of contact time with soap. Know the difference of bacteria on the variation of concentration of lime soap and soap without lime. The study used the experimental method with pretest and posttest with control design. The independent variables are a lime soap with a concentration of 40%, 50%, 60% and variable of contact time 30 second, 60 second and 90 seconds. The dependent variable is the number of bacteria on the dinnerware of a dinner plate. The data obtained in this study is the data of measurement of the number of bacteria on the dinner plate. Data analyzed by Kruskal-Wallis test. MPN coliform examination of river water showed results >2400 CFU/ml sample. There were no significant differences in treatment with contact time variation of 30 seconds, 60 seconds and 90 seconds. The significance value is 0.796 (> 0.05). There were significant differences in the treatment of lime soap with concentration variations of 40%, 50%, 60%, and soap without lime. The value of significance is 0,000 (<0.05).

Blood Glucose Level And Candida spp. Growth In Elderly Diabetes Melitus Patients Rifqoh Rifqoh; Syaidatul Aslamiah; Jujuk Anton Cahyono; Erpan Roebiakto
Medical Laboratory Technology Journal Vol 4, No 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Diabetes mellitus is a presdisposing factor against infection, especially in orofacial area. Infectious diseases are more frequent serious in patients with diabetes mellitus, which potentially increases their morbimortality. The more frequent oral mucosa infection of DM patients is candidiasis which caused by Candida spp. This research is aimed to obtain the correlation between fasting blood glucose level and Candida spp. growth in elderly diabetes mellitus patients. This analytical survey research used cross-sectional design through GOD-PAP methode for fasting blood glucose level test and macroscopic method for total colony of Candida spp. growth. The result of fasting blood glucose level test average 186,06 mg/dl the highest level is 492,90 mg/dl and the lowest one is 72,08 mg/dl. Meanwhile, the result of Candida spp. is 131 at the average and the highest growth is 350 and the lowest one is 12 colonies. The result of the research indicates that there is a rising of Candida spp. to level of fasting blood glucose in elderly diabetes mellitus patients. Based on correlational test of spearman, it gains significant value 0,001 < α = 0,005 of that shows there is a correlation between fasting blood glucose level and Candida spp. growth in diabetes mellitus elderly patients with the strenght relation (r) = 0,572 which means in medium level. Further researches about the influence to the other presdisposing factors of Candida spp. growth are recommended

Correlation Between Onset of Diabetes Mellitus and Nitric Oxide Levels in Patient with Type 2 Diabetes Mellitus Theosobia Grace Orno; Mansyur Arif; Irfan Idris
Medical Laboratory Technology Journal Vol 4, No 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Type 2 diabetes mellitus (DM) is associated with increased risk of endothelial dysfunction if it lasts a long time without control. This study aims to connect the Onset of Diabetes Mellitus (DM) with Nitric Oxide levels in patients of type 2 diabetes mellitus. The study used cross-sectional study method. The samples were 86 subjects, consisting of 38 subjects of Type 2 DM controlled and 48 subjects of Type 2 DM uncontrolled. The results of the Kruskal-Wallis statistical test showed no significant difference between the Onset of DM and Nitric Oxide levels in the categories of 4-6 years (19.4 ± 10.1), 7-9 years (17.3 ± 9.3) and 10-12 years (13.3 ± 8.5) (p=0.06). Furthermore, the Spearman correlation test revealed a negative correlation between the Onset of DM and Nitric Oxide level in patients with Type 2 DM with and without control (r =-0.217). The level of Nitric Oxide (NO) can consider as a predictor of long-term complication in patients with type 2 DM.

Effect of Active Carbon of Coffee Robusta Waste (Coffea robusta Lindl.) in Reducing Iron of Peat Water Dinna Rakhmina; Rizki Mulanova; Haitami Haitami
Medical Laboratory Technology Journal Vol 4, No 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Peat water has a distinctive dark brown or yellow color, low pH, it tastes sour, high organic matter content, high levels of iron and manganese. One of the ways that can be used to reduce the levels of iron in peat water is doing adsorption process by using activated carbon coffee waste because the coffee waste included organic ingredients. The purpose of this research was to identify the influence of the concentration of activated carbon robusta coffee waste (Coffea robusta Lindl.) against the iron levels in peat water. This research uses the draft Posttest-Only Control Group Design. Manufacture of activated carbon robusta coffee waste (Coffea robusta Lindl.) consists of the processes of dehydration, carbonization, and activation, with the results 25.9% of rendemen, 14.05% water content, a rate of 9.9% ash, and iod absorbance 647.19 mg/g. Activated carbon coffee waste added to 100 ml of peat water as much as 3 gr, 4.5 gr, and 6 gr with the stirring speed 400 rpm for 60 minutes obtained early iron levels of 2.75 mg/L and dropped to 1.19 mg/L, 1.02 mg/L, and 0.95 mg/L. Conclusions This study is there is the influence of the concentration of activated carbon robusta coffee waste (Coffea robusta Lindl.) against the iron level of peat water iron and frequency of 6 gr as a concentration of the best efficiency adsorption of 65.4% because it can reduce the levels of iron in 100 ml of peat water from 2.75 mg/L be 0.95 mg/L. By regulation of the Minister of health RI No. 416/Menkes/PER/IX/1990 namely of 1.0 mg/L. Suggestions for further research to improve the heating temperature and concentration of the Activator to obtain active carbon absorption.

Staphylococcus aureus and Methicillin resistant Staphylococcus aureus From The Diabetic Ulcer Leka Lutpiatina; Nisa Nur Agistni Eriana
Medical Laboratory Technology Journal Vol 4, No 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Infections of diabetic ulcers may occur because patients with diabetes mellitus have a weak immune system and the presence of high blood sugar into a strategic place for bacterial growth one of them is Staphylococcus aureus. Increased resistance of Staphylococcus aureus to various antibiotics, causing problems for the treatment of this infection. As by Methicillin-resistant Staphylococcus aureus (MRSA) This study aims to assess the presence of Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) at diabetic ulcer patient at Banjarmasin city hospital. This research uses descriptive observational research type. The study sample was patients with grade 2 and three diabetic ulcers with a total of 30 samples. The result of the research found 14 Staphylococcus aureus bacteria, Staphylococcus epidermidis nine, Staphylococcus saprophyticus two and 5 Gram-negative bacteria. Of the 14 samples of Staphylococcus aureus, there were eight samples of cefoxitin-resistant samples. The conclusion was 47% of Staphylococcus aureus in patients with a diabetic ulcer at Banjarmasin City Hospital. There was a 57% Methicillin-resistant Staphylococcus aureus in the isolated Staphylococcus aureus found. Need further research on the resistance of antibiotics oxacillin and cefoxitin to other Staphylococcus sp bacteria derived from patient diabetic ulcers.

Sputum Quality of The Anytime And Outset for Examination Acid-Resistance Bacilli Leka Lutpiatina; Wahidah Wahidah; Nurhilaliah Nurhilaliah; Dinna Rakhmina; Rifqoh Rifqoh
Medical Laboratory Technology Journal Vol 4, No 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Diagnosis Tuberculosis (TB) can establish by microscopic examination of acid-resistant bacilli in the patient's sputum. Sputum quality greatly affects the diagnosis of TB, but the sputum collected by patients at community health centers sometimes does not meet the sputum quality requirements. The objective of this study was to assess the quality of sputum anytime and outset sputum on acid-resistant bacilli examination, from volume, color and viscosity parameters. The type of research used is descriptive observational. Respondents were suspected TB patients at the Marabahan and Aluh-aluh Public Health Centers of South Kalimantan province, Indonesia. Sputum samples taken were sputum anytime the first and outset sputum. The variables in this study are volume, color, and viscosity of sputum. The coloring of acid-resistant bacilli method Ziehl Nielsen. The results of the acid-resistant bacilli examination on sputum (86 specimens) found positive one (13%), positive three (2%), negative (85%). In morning sputum (86 specimens) found positive one (14%), positive three (2%), negative (84%). Sputum quality when with the good category as much as 36% and the quality of morning sputum with the good category as much as 55%. Conclusion Sputum outset has better quality than sputum at the anytime.

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