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Medical Laboratory Technology Journal

JAK
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Published by Poltekkes Kemenkes Banjarmasin

ISSN : - EISSN : 24610879 DOI : https://doi.org/10.31964/mltj

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health

The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 157 Documents

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Akurasi Hitung Jumlah Eritrosit Metode Manual dan Metode Otomatis Neni Oktiyani; fahriyan fahriyan; Akhmad Muhlisin
Medical Laboratory Technology Journal Vol. 3 No. 2 (2017): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

In a laboratory, an erythrocyte is done by using hemocytometer and microscope. The task is to measure and assess the size and shape of the erythrocyte. But this procedure is time consuming, complex and tedious. As a solution to this problem, to provide an automated, cost-effective and efficient alternative to detection and counting of erythrocyte, hematology analyzers are used. However, false results related either to erythrocyte or other parameters from complete blood count may be observed in several instances. The objective study was to compare the accuracy of erythrocyte count results of automatic hematological analysis by the manual method. The study is an analytic survey with a cross-sectional design. Erythrocyte counting is done by using three types of control blood, namely high, normal and low, with 9 repetitions. In high control blood, the mean erythrocytes count by the manual method was 7.08 million/μl with a bias value of 1.4%, while that by the automated method was 7.03 million/μl with a bias value of 0.7%. The mean erythrocyte count in normal control blood by the manual method was 4.50 million/μl with a bias value of 0.9%, while that by the automated method was 4.4 million/μl with a bias value of 2.4%. And in low control blood, the mean erythrocyte count by the manual method was 1.72 million/μl with 4.4% bias value, while that the automated method was 1.67 million/μl with 1.2% bias value. Statistical analysis showed no significant difference in accuracy between the erythrocyte count of manual methods with automated methods.

Efektivitas Air Rebusan Daun Binahong (Anredera cordifolia) Terhadap Pertumbuhan Salmonella typhi Ratih Dewi Dwiyanti; Nurlailah Nurlailah; Indah Kurnia Widiningsih
Medical Laboratory Technology Journal Vol. 1 No. 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Typhus is one of acute febrile illness caused by the bacterium Salmonella typhi. Treatment of typhoid fever usually use antibiotics, the use of antibiotics can cause side effects. People today are using treatment with natural ingredients, one of which is Binahong (Anredera cordifolia) compounds containing alkaloids, polyphenols, flavonoids, saponin, and anthraquinone is efficacious as an antibacterial. This study aims to determine the effectiveness of the water decoction of leaves Binahong against Salmonella typhi growth in vitro. This type of research is true experiment with posttest study design Only Control Group Design and methods used are diffusion (wells) with 5 treatment. The concentration of the cooking water leaves the dgunakan Binahong is 20%, 40%, 60%, 80% and 100%. The result showed inhibition zone water decoction of the leaves Binahong against Salmonella typhi at a concentration of 20%, 40%, 60%, 80% is 0 mm, whereas at 100% concentration obtained inhibition zone of 11 mm. It is concluded that the water decoction of the leaves Binahong at a concentration of 100% has the ability to inhibit the growth of Salmonella typhi, but these results have not been effective because it is still in the category of resistance. It is suggested for further research to increase the concentration of water decoction of the leaves binahong or use alcohol extract of leaves binahong to inhibit the growth of Salmonella typhi.

Peran Ekstrak Daun Pepaya (Carica papaya Linn) terhadap Kematian Larva Nyamuk Aedes aegypti Deby Swastika; Lenie Marlinae; laily khairiyati
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Papaya plants contain many substances called papain, papain protease effect can kill larvae of Aedes aegypti. This study aimed to determine the effect of papaya leaves extracts (Carica papaya Linn) against Aedes aegypti larvae mortality. The research method was a quasi-experimental research design with Non-Equivalent Control Group. The concentration levels were 6.25%, 12.5%, 25%, 50%, 100%, and controls (CMC-Na) with 4 times replication. The Probit Analysis test results showed Lethal concentration (LC50) of papaya leaves extract (Carica papaya Linn) against Aedes aegypti larvae mortality was to 24.46% and the effective time is 22 hours. It is concluded that any different concentrations of papaya (Carica papaya Linn) leaves extract have effect on Aedes aegypti larvae mortality (p

Konsumsi Buah Teratai (Nymphea sp) Sebagai Determinan Terjadinya Fasciolopsis Buski pada Anak Muhamad Muslim; Rifqoh Rifqoh; Irwandi R
Medical Laboratory Technology Journal Vol. 2 No. 1 (2016): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Fasciolopsis buski is a health problem that is difficult to eradicate. Fasciolopsis buski infection is caused by ingested metacercariae that live on aquatic plants. One factor that supports the transmission mechanism of Fasciolopsis buski, of which there are still some people, especially children who have a habit of eating Nymphea sp. The study aims to determine the relationship with lotus fruit consumption habits with Fasciolopsis buski infection. The type of research is analytic survey with cross sectional approach, the study samples are 69 students of Sungai Papuyu primary school consists of 38 men and 31 women, the technique of sampling is random sampling (simple random), research instruments are using questionnaires and microscopic examination of worm eggs, data analysis is using the Chi-square. Students are accustomed to eating Nymphea sp of crude (not good) are 31 people (44.93%), and are not familiar (well) are to 38 people (55.7%). A total of 7 people (10:14%) are infected Fasciolopsis buski and 62 (89.86%) are not. Students who are infected Fasciolopsis buski entirely used to eat fruit lotus. Based on the statistical test Chi-Square, there is a relationship between the habit of eating Nymphea sp with lotus Fasciolopsis buski infection with the value of Fisher's Exact Test 0,002 less than the value ? of 0.05. The habit of eating Nymphea sp are associated with infections to the students.

Potensi Antifungi Tangkai Daun Jarak Pagar Terhadap Pertumbuhan Candida albicans Ni Made Niagita Wiratni; I Nyoman Jirna; IGA. Sri Dhyanaputri
Medical Laboratory Technology Journal Vol. 3 No. 2 (2017): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Jatropha curcas twigs are part of the Jatropha curcas which can be utilized as herbal treatment by the community to overcome the vaginal discharge problem. Vaginal discharge is a common symptom experienced by most women caused by Candida albicans infection. This research used the true experiment with posttest only control design which aimed to know the content of phytochemistry and antifungal potential of Jatropha curcas twigs extract on Candida albicans growth. Extract of Jatropha curcas in this study was obtained through a solvent extraction process using ethanol 96% by the method of maceration. The method used for the phytochemistry test was a qualitative method, while for the antifungal potential test was done by diffusion method with the concentration of 10%, 25%, 30%, 40% and 50%. The results of Phytochemistry test showed that Jatropha curcas twigs extracts are saponins, tannins, and flavonoids, but no alkaloid compounds were found, however a test result of the antifungal potential test showed that the average diameter of inhibition zone of Candida albicans growth is 0 mm. The conclusion of this study is Jatropha curcas twigs extracts can not inhibit Candida albicans growth, For other researchers which use jatropha curcas twigs, are suggested to do a phytochemical test quantitatively and to test the antifungal potential with dilution method.

Pengaruh Kalsium Klorida (CaCl2) dan Lama Penyimpanan terhadap Kadar Vitamin C Anggur (Vitisvinifera) Anny Thuraidah; haitami haitami; Akhmad Dairobi
Medical Laboratory Technology Journal Vol. 1 No. 2 (2015): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Vitisvinifera is one of the perishable fruit and not durable when stored at room temperature. To maintain the levels of vitamin C wine then used treatment using calcium chloride (CaCl2). This study aims to determine the effect of treatment Calcium chloride (CaCl2) and duration of storage of the vitamin C content of Vitisvinifera This type of research uses experimental methods actual (true experiment) in the form of draft posttest only control group design. Wine and CaCl2 research materials. Data were obtained by performing assays using titration iodometry vitamin C in grapes. Results of statistical analysis to test multiple linear regression R2 values obtained showed no effect of treatment 0.578 Calcium chloride (CaCl2) and storage time for blood levels of vitamin C Vitisvinifera. From Duncan statistical test obtained significant value for all subsets of the 2 treatment groups of more than 0.05, the most optimal levels of vitamin C in maintaining the Vitisvinifera is a CaCl2 concentration of 0.05 M for 4 days of storage. Therefore, this study suggested as alternative preservation for merchants and producers / grape growers in order to maintain the vitamin C content of wine so as to improve the quality and the quality of the fruit.

Tinjauan Kualitas Bakteriologis dan Tingkat Risiko Pencemaran Air Sumur Gali di Kelurahan Sungai Ulin Kota Banjarbaru Erpan Roebiakto; Gunung Setiadi; Yohannes Joko Supriyadi
Medical Laboratory Technology Journal Vol. 3 No. 1 (2017): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Humans in meeting the needs of water should pay attention to aspects of quality and quantity. Particularly the aspect of bacteriological quality, because contaminated water can cause waterborne disease. One of the clean water facilities used by the community is the well. The community of Sungai Ulin Kota Banjarbaru uses wells for daily activities. The purpose of this research is to know the bacteriological quality and the level of risk of water pollution wells Sungai Ulin Kelurahan Kota Banjarbaru. The research type is descriptive and cross-sectional research design. The population of this study was all wells in three Rukun Tetangga (RT 19, RT 20 and RT 21) of 40 and samples of bacteriological quality of well good water. The result of the research is the characteristic of respondent from the level of education of most elementary school is 19 (47.5%), knowledge level 21 (52,5%), middle income category (1-3 million / month) 36 (90%) and length of stay Respondents old category (> 10 years) as many as 39 (78%). The conclusion of the study showed that the quality of bacteriologic (MPN Coli) of well water 3 (7.5%) was eligible and 37 (92.5%) were not eligible. The level of risk of contamination of wells to 40 wells, among others, for very high risk of 6 units (15%), high risk of 26 units (65%), medium risk 5 pieces (12.5%) and low risk 3 (7.5%). Need to do research about the relationship of good construction with bacteriological quality of good water.

Kulit Pisang Kepok (Musa paradisiaca L.) untuk Menurunkan Kadar Mangan Air Sumur Anny Thuraidah; jasmadi Joko Kartiko; Lailan Febry Ariyani
Medical Laboratory Technology Journal Vol. 1 No. 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Abstract: Water is an essential requirement. Part of Indonesian people still using well as the source of water Resulting from the increased of human activity and industries, the well water maybe contaminated by manganese ( Mn). That is needed processing to make well water usefull for mankind. One of the type for processing water is the absorbtion methode .Banana shell was kitchen waste and the dried of Kepok Banana (Musa paradisiaca) Shell was used as adsobent to reduce Manganese level in water. This study aimed to determine the reduce of Manganese level in water. This research was real experiment pretest and posttest study design with a control group. Persulfate research instruments colorimetric and spectrophotometric measurements. The addition of the dose banana shell were 0,06 ; 0,07; 0,08; 0,09; 0,1 gram . By those dose cumulatively decreased to 0; 21,84; 32,34; 44,56; and 52,92%. The Manganese maximum reduced by 0,1 gram dried banana shell in 100 cc well water in 30 minutes. Stastically , the significan value was 0,000 less than 0,05(α) , it was meant that the variety dose of dried Kepok banana shell influence to decrease Manganese level in water. These decreased way can be used as an alternative to increase the water quality.

Kadar Sianida Singkong Rebus dan Singkong Goreng Yeni Purwati; Anny Thuraidah; Dinna Rakhmina
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Cassava is the third staple food often used by people to make a variety of products. Carbohydrates from cassava also contains toxic compound such as cyanide which can inhibit the action of respiratory enzymes. Cyanide in cassava can be removed by boiling or frying process. The aim of research was to find out the cyanide level differences between boiled cassava and fried cassava. This type of research was experiment, with design of comparative studies approach. Cyanide levels were examined in 10 samples with 2 treatments using Argentometry Volhard titration method. The result showed the average of cyanide levels in boiled cassava was 0.772 ppm and in fried cassava was 1.069 ppm. The percentage of cyanide levels reduction in boiled cassava was 28,78%, while for the fried cassava was 0%. The statistical test obtained the value of p

Pengaruh Lama Penyimpanan Urine pada Suhu Kamar terhadap Jumlah Leukosit Studi pada Penderita Diabetes Melitus Yayuk Kustiningsih; Jujuk Anton Cahyono; Nur Rahmiati
Medical Laboratory Technology Journal Vol. 2 No. 1 (2016): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Abstract: Examination of urine leukocyte count usually use urine samples during and urine examination is performed while still fresh because elements in the form of urine begins to break down within 2 hours. The purpose of this study was to determine the effect of storage time on the number of leukocytes urine checked immediately (0 hours) and were kept 30, 60, 90, 120, 150 and 180 minutes at room temperature. Types of Research true experiment to investigate the result of a treatment, research conducted on four samples of urine of patients with diabetes mellitus who meet several criteria, namely blood glucose levels> 200 mg / dl and urine amount leukositnya> 30 cells / mm3, calculated using the Improved Neubauer . The result showed a decrease in the number of stored urine leukocytes 30 minutes = 10%, 60 min = 25%, 90 min = 32%, 120 minutes = 39%, 150 minutes = 43%, and 180 minutes = 51%. Values obtained by linear regression test p = 0.034 (

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ricko Dharmadi Utama

Contact Email apinaga53@gmail.com

Phone +6282256602225

Journal Mail Official editormltj@gmail.com

Editorial Address Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location Kota banjarmasin, Kalimantan selatan INDONESIA

Abstract

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Abstract

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Abstract

Abstract

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Abstract

Contact Name
Ricko Dharmadi Utama

Contact Email
apinaga53@gmail.com

Phone
+6282256602225

Journal Mail Official
editormltj@gmail.com

Editorial Address
Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location
Kota banjarmasin,

Kalimantan selatan

INDONESIA