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THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

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Merupakan media publikasi ilmiah yang memuat berbagai artikel ilmiah hasil tinjauan pustaka, dan hasil penelitian dalam bidang ilmu Patologi Klinik, Hematologi, Mikrobiologi, Parasitologi, Imunologi, Sitohistoteknologi, Kimia Kesehatan dan Ilmu Teknologi Laboratorium Medik

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UJI ANTIBAKTERI PERASAN AKAR BAYAM DURI (Amaranthus spinosus Linn) TERHADAP Shigella dysenteriae Ariana, Diah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Edisi 1: The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

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UJI ANTIBAKTERI PERASAN AKAR BAYAM DURI (Amaranthus spinosus Linn) TERHADAP Shigella dysenteriaeDiah Ariana, S.T. M.Kes.Prodi D3 Analis Kesehatan UM Surabayadiahariana0101@gmail.comÂ ABSTRACTShigella dysenteriae is a bacterial pathogen that causes shigellosis causes severe diarrhea, prolonged, and fatal compared with diarrhea caused by other Shigella. The use of antibacterial drugs in the form of synthetic chemistry with a long period of time can cause side effects such as allergic reactions, asthma, and indigestion. Thorny Amaranth Root (Amaranthus spinosus Linn) is one of the traditional herbs that can be used as a natural antibacterial against Shigella dysenteriae because it contains flavonoids, alkaloids, and tannins so that the plants do not cause side effects.The formulation of the problem of this research is there any effect of freshly squeezed Thorny Amaranth Root (Amaranthus spinosus Linn) against Shigella dysenteriae. The purpose of this study to investigate the effect and the optimum concentration Thorny Amaranth Root juice (Amaranthus spinosus Linn) against Shigella dysenteriae. This type of research is experimental with a total of 28 samples consisting of 7 concentrations, ie 0%, 50%, 60%, 70%, 80%, 90% and 100% and 4 repetitions. This research is conducted in the Laboratory of Microbiology Prodi D3 Health Analyst Faculty of Health Sciences, University of Muhammadiyah Surabaya for 3 days. Using a dilution method by observing the presence or absence of bacterial growth. Analysis of data using ANOVA test with an error rate of 5% (0.05). And advanced test using test Tukeys Honest Significant Difference (Tukeys HSD). The final conclusion is that there is influence Thorny Amaranth Root (Amaranthus spinosus Linn) against Shigella dysenteriae. After testing Tukeys HSD obtained Minimum inhibitory concentration (MIC) is 80%.Â Keywords: Shigella dysenteriae, Amaranthus spinosus Linn

Pengaruh Hemolisis Terdapat Kadar Serum Glutamate Pyruvate Transaminase (SGPT) Sebagai Salah Satu Parameter Fungsi Hati hartono kahar
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

AbstractSGPT examination is one of the important checks on the state of a particular patient, for example, in patients with liver disease, hepatitis, and so forth. The other purpose is to detect the presence of liver damage. So there was an effect in the event of an increase in maupaun drop in levels. And the serum circumstances that do not qualify, such as serum hemolysis, will greatly affect the actual levels of ALT. Based on the research background, the authors formulate the problem as follows: "Is there any effect of hemolysis on SGPT levels as one of the parameters of liver function?". The purpose of this study is to prove whether there is an influence of hemolysis on SGPT levels as one of the parameters of liver function. The sample was 20 samples and 20 samples of hemolysis hemolysis. Location and time of the research conducted at Muhammadiyah Hospital Clinical Laboratory Lamongan ie in January and July. Of the 20 samples analyzed, showing that levels higher than the hemolysis ALT SGPT levels no hemolysis. When the serum through lysis will experience a significant increase. To test the effect of hemolysis on SGPT levels paired t test was used because the data from this study with normally distributed. The results of a paired t test analysis between serum hemolysis and no hemolysis on levels of serum ALT levels showed a significant value 0.00 thus P <0.05 it can be concluded that there is no difference between the levels of ALT SGPT levels of hemolysis with hemolysis. That means there is the influence of hemolysis on SGPT levels as one of the parameters of liver function. Key word : hemolysis, glutamate pyruvate transminase

Perbedaan Zona Hambat Terhadap Jamur Malassezia Furfur Antara Pemberian Ekstrak Umbi Bawang Putih (Allium Sativum Linn) Dengan Ekstrak Kulit Umbi Bawang Putih (Allium Sativum Linn) Diah Ariana
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Tinea versicolor or pitiriasis versicolor is a skin disorder caused by Malassezia furfur infection which is a fungal infection on the skin of the chronic surface. Clinical features is in the form of fine spots that colored white to brown black, this infection is also called a skin fungus with blotches. Treatment of Tinea versicolor can use chemical or traditional medicine. Chemical treatments such as creams or ointments cause sticky taste, while one of the traditional medicine is the bulb and skin of the garlic bulb (Allium sativum Linn) containing allicin substances that are efficacious as an anti-fungal. This research is an experimental research with the aim to know the difference of inhibition zone to Malessezia furfur fungus. There are 3 treatments with 9 repetitions. The extraction method used is the soxhlet method. Anti-fungal activity test was done by using rating method, Saboroud Dextrose Agar (SDA) media which has been inoculated with suspension of Malassezia furfur with concentration 1.5x108 CFU/ml (Mc Farland standard). Result of data analysis obtained by ANOVA p = 0,000 < α = 0,05, that there is difference of inhibition zone from treatment of garlic bulb extract and skin of garlic bulb (Allium sativum Linn). It is indicated that there is effect of giving garlic bulb extract (Allium sativum Linn) and garlic bulb skin (Allium sativum Linn) to Malassezia furfur fungus. Further tests were performed to compare mean treatment i.e. HSD test. It was obtained sig (p) 0,016 > α 0,05 which means bulb extract and garlic bulb skin (Allium sativum Linn) have different effect. Keywords: Tinea versicolor, Malassezia furfur, garlic bulb.

Efek Anti Oksidan Bawang Putih terhadap Penurunan Bilangan Peroksida Minyak Jelantah Siti Mardiyah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Used cooking oil is leftover cooking oil from food frying processes. The use of cooking oil which is repeated with a high temperature and a long time can cause the oil to oxidation and hydrolysis, resulting in increased levels of peroxide. High peroxide value can pose a health hazard. Allisin is antioxidants contained in garlic. Allisin are primary antioxidants which act as free radical scavengers that can slow oxidation on used cooking oil. The purpose of this study was to determine the effect of garlic on decreased levels of peroxide in used cooking oil. This type of research is experimental by the number of samples in each treatment as many as 4 samples. Formulation of the problem of this study is whether there is the effect of adding garlic to decreased levels of peroxide on used cooking oil.From the results of the average levels of peroxide in used cooking oil that has been saved within 3 days is 26.0482 control mEq, 10 mEq% 23.65098, 22.71355 mEq 20%, 30% mEq 18.65023, 17.92128 mEq 40%, 50% 16.52508 mEq. Statistical analysis with ANOVA test shows the effect of adding garlic to decreased levels of peroxide in used cooking oil with p value (sig) = 0.000 which is smaller than 0.05. The results of Tukey HSD test showed the addition of garlic 30% is effective to reduce levels of peroxide on used cooking oil.Therefore, the addition of garlic may inhibit the process of rancidity and lower levels of peroxide in used cooking oil.KEYWORDS: garlic, cooking oil, peroxide value

Perbandingan Pemeriksaan Leukosit Urine Segar Dengan Setelah 2 Jam Di Suhu Kamar Nur Vita Purwaningsih
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

ABSTRACTBackground: Urinalysis is a parameter often requested by clinicians. The urinalysis parameters consist of macroscopic, microscopic or sedimental examination and urine chemistry examination. Urine examination is very important, especially in making the diagnosis. Procrastination delay results in errors in diagnosis and administration of drugs that lead to adverse outcomes of patients, analysis should be performed no later than 4 hours after sampling. Urine has a stability at room temperature ie for 1 hour, if urine is silenced long then the bacteria will multiply, so it can decompose NH3 (ammonia) which is alkaline. Under alkaline conditions, the pH in the urine will increase. This may affect the sediment component in the urine to rapid lysis so that the amount will be reduced General purpose of this study to determine the difference results of examination of fresh urine leukocytes compared after 2 hours at room temperature. Method: descriptive observasional with number of sample counted 20 sample. Result: Based on the test result, the positive percentage value in fresh urine is 100% and the positive percentage value in urine after 2 hours is 70%. In the Wilcoxon statistical test obtained p-value <0.001 (> 0.05). Conclusion: Based on the research that has been done then it can be concluded that there are differences in the results of fresh urine leukosit better than urine after 2 hours at room temperature.Keywords: Leukocyte urine, Fresh urine, delay.

Hubungan Kadar TSH Terhadap Kadar FT4 Pada Pasien Tiroid Di Bangkalan Ellies Tunjung Sari Maulidiyanti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

AbstractThyroid disorder disease ranks second most in the list of metabolic diseases after diabetes mellitus (DM). Thyroid disease or Thyroid abnormalities is a condition of abnormalities in a person due to the disorder of the thyroid gland. TSH is a primary factor that controls thyroid cell growth and synthesis also thyroid hormone secretion. TSH secretion is stimulated by low levels of T3 and T4 and by the hormone TRH (Thyroid Releasing Hormone) the hypothalamus is inhibited by elevated levels of T3 and T4. T3 and T4 are circulating in the plasma, most of which are bound with proteins, Thyroid Binding Globulin (TBG) and a small part in free form that are Free Triiodotironine (FT3) and Free Thyroxine (FT4). The free hormone (FT3 and FT4) is an actively metabolic fraction which is known to be quantitative. Currently the measurement of free hormone levels should be part of a complete examination of the status of the thyroid gland. The purpose analyzes the levels of TSH, FT4 and proves the relationship between the two in patients with thyroid disorders. This examination by using ELISA method is with EIA competitive principles and sandwiches. The samples are serum of patients with thyroid disorders at RSUD Bangkalan and Farmalab Laboratory from October to November 2017.The 30 samples obtaines an average result of TSH level of 1.9 mIU / L and an average FT4 level of 2.8 ng / dl. Next test Kolmogorov-Smirnov and Spearrman's test results obtained if the variable one value rose (high) then the other variable down (low). It can be concluded that there is an opposite relationship between TSH levels and FT4 levels, if TSH levels increase, the FT4 level decreases, and so it is also in contrast Keywords: TSH, FT4, thyroid disorders

Perbedaan Pertumbuhan Bakteri Shigella dysentriae Pada Berbagai Konsentrasi Perasan Kulit Apel Manalagi (Malus sylvestris Mill) Secara In Vitro Dita Artanti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Apple manalagi is one type of apple that is consumed by many Indonesian people, because it tastes sweet, tasty, easy to get and the price is quite affordable. The Manalagi apple rind contains some phytochemical derivatives of polyphenols that have antibacterial effects. Shigella dysentriae is a bacillus bacteria that causes acute diarrhea (dysentery) in most people who lack hygiene. One alternative to prevent the disease is to use natural ingredients as an antibacterial. The aim of this research is to know the different growth of S. dysentriae bacteria in various concentrations of Manalagi apple rind (Malus sylvestris Mill.) In vitro. The type of this study was experimental consisting of 7 treatments with 4 repetitions. The sample consisted of 7 concentrations of manalagi apple rind (100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 0% (control) .The data analysis used ANOVA test with 5% 0.05). The results showed that the growth of S.dysentriae bacteria was only at 0% concentration; 3.125%; 6.25%; 12.5%; and 25%. While at concentrations of 50% and 100% there was no growth of S.dysentriae bacterial colonies. The conclusion of this study proves that Manalagi apple rind has antibacterial power against S.dysentriae bacteria.Keywords: Shigella dysentriae, Manalagi Apple Rind (Malus sylvestris Mill).

Gambaran Histologi Ginjal Tikus Wistar Yang Terpapar MSG Setelah Perlakuan Diberikan Jus Tomat Dan Diberhentikan Perlakuan Saja Yeti Eka Sispitasari
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

MSG causes many side effects on the body, but MSG has long been used as a food flavor that can bring tastes (umami) and play a role in strengthening the taste. The chemical structure of MSG is no different from Glutamic Acid (glutamate), it is one of the 20 amino acids that make up proteins in the body. So the use of MSG needs to be discontinued to prevent kidney damage due to continued consumption of MSG, two research were conducted to determine the effect of discharging of MSG consumption by being given tomato juice (1) and discharged without treatment (2), the second study was seen from the histology picture of rats wistar mouse.Methods were experimental with post test only control group design. The research (1) used 15 wistar rats divided into three groups: group I without treatment (pellet AD II and drinking water), group II was given MSG for 14 days and group III was given tomato juice and MSG for 14 days. The study (2) used 27 rats divided by 9 groups. The sample is selected by simple random sampling method. The mice were then gradually turned off on the 29th, 43rd and 57th days. There was no significant difference in mean number of normal and damaged proximal tubules in all treatment groups. The renal histologic features in the treatment group I (MSG) and the treatment group II (MSG and tomato juice) showed normal glomeruli, tubular epithelial tubules, and lumen tubular narrowing whereas in mice discharged without treatment there was no apparent difference between the two MSG Giving groups damage to proximal tubules and renal corpusculum and regeneration after 14 days of discontinuation of MSG. Conclusions from these two experiments occurred damage to the renal tubules Keywords: MSG, Kidney, Wistar Rat

Respon Inflamasi Pada Perokok Pasif Di Kecamatan Pahandut Kota Palangka Raya Ditinjau Dari Jumlah Leukosit Dan Jenis Leukosit rinny ardina
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Proporsi perokok pasif di Indonesia mencapai 40,5 persen dan 78,4 persen perokok pasif banyak terpapar asap rokok di dalam rumah.Setiap hembusan asap rokok mengandung 1015 radikal bebas oksidatif dan nikotinpenyebab leukositosis di dalam darah sebagai bentuk respon inflamasi.Jenis leukosit penunjuk inflamasi akibat asap rokok diawali neutrofilia, neutropenia, limfositosis, limfopenia dan monositosis sebagai bentuk respon inflamasi. Analisis deskriptif dengan rancangan cross sectional digunakan untuk memberikan gambaran respon inflamasi ditinjau dari jumlah leukosit dan jenis leukosit pada perokok pasif yang tinggal di kecamatan Pahandut kota Palangka Raya. Sampel yang diperoleh sebanyak 30 orang diambil dengan teknik Purposive Sampling dimulai dengan observasi, pembagian lembar inform consent, wawancara, pengisian kuesioner, pengambilan sampel darah dan pemeriksaan laboratorium menggunakan hematology analyzer dan apusan darah tepi. Data yang diperoleh dimuat dalam bentuk tabel dan dianalisis dan dideskripsikan dalam bentuk persentase (%). Pada 5 orang (17%) terjadi leukositosis, 2 orang (6%) neutropenia, 1 orang (3%) neutrofilia, 1 orang (3%) limfopenia, dan 6 orang (20%) limfositosis. Leukositosis menunjukkan penanda adanya perubahan imun sistemik, dimana selanjutnya terjadi rekrutmen sel inflamasi diantaranya neutrofil dan limfosit. Radikal bebas dan nikotin memicu inflamasi ditandai dengan neutrofilia dengan limfopenia relatif pada awal inflamasi dan selanjutnya berkembang menjadi limfositosis dengan neutropenia relatif.Proportion of passive smokers in Indonesia reached 40,5% and 78,4% of them were exposure of cigarette smoke in the house. Each puff of cigarette smoke contains 1015 of oxidative free radicals and nicotine that causes leukocytosis in the blood where it found as an inflammatory response. Either free radicals or nicotine can also cause of neutrophilia, neutropenia, lymphocytosis, lymphopenia, and monocytosis. This study aimed to overview of the inflammatory response in terms of white blood cell count and differential counting of leukocytes in passive smokers (housewives) who live in the district of Pahandut, Palangka Raya. Descriptive analysis with cross sectional design was used in this study. Samples obtained by 30 people were taken with Purposive Sampling technique. Procedure of collecting data starts from observation, distribution of informed consent, interview, answered of questionnaire, blood sampling and laboratory examination using hematology analyzer for measured of white blood cell count and peripheral blood smear for measured of differential counting of leukocytes. The data obtained were analyzed and described in percentage (%). This study found 5 people (17%) of leukocytosis, 2 people (6%) of neutropenia, 1 person (3%) of neutrophilia, 1 person (3%) of lymphopenia, and 6 people (20%) of lymphocytosis. Leukocytosis occurs because of the mechanism of leukocytes recruitment into inflammatory tissues and the types of leukocyte that increased are neutrophils and lymphocytes. Free radicals and nicotine play a major role in triggering inflammation. Acute inflammation cause of exposure of cigarette smoke characterized by neutrophilia and relative lymphopenia in the blood and it will be develop into chronic inflammation that characterized by lymphocytosis and relative neutropenia.

Variasi Kandungan Merkuri (Hg) Pada Berbagai Macam Bedak Whitening Yang Dijual Pasar Blauran Surabaya Nastiti Kartikorini
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Mercury is widely used in cosmetics to remove black spots on the face. Mercury in the smallest amount can be a toxic such as skin discoloration, black spots, allergies and irritation. High doses of mercury can cause permanent damage to the brain, kidneys and impaired development of the fetus,distruction skin surface.The purpose of this study was to analyze mercury levels in various brands of whitening powder sold in Blauran market, Surabaya. The samples were 22 types of whitening powder. The samples were analyzed by Atomic Absorption Spectrophotometer (AAS).According to the results of research on mercury content (Hg) of 22 whitening powder, it is known that 4 whitening powder contain mercury (Hg), and have mercury (Hg) which is vary from the highest level (0.016 ppm) and the lowest level (0,008 ppm). However, the mercury content (Hg) is still below the maximum limit set. Keywords: Whitening Powder, Mercury, Atomic Absorption Spectrophotometer (AAS)

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