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Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
ISSN : 20895690     EISSN : 24069272     DOI : -
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
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Articles 1 Documents
Search results for , issue " Vol 12, No 3 (2017): December 2017" : 1 Documents clear
Fungal Community Structure of Macroalga Ulva Intestinalis Revealed by Miseq Sequencing Agusman, Agusman; Danqing, Feng
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 3 (2017): December 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i3.315

Abstract

Since algae are well known as the host of fungi, the information about total fungal community associated with alga is needed to give the initial data that is useful for natural product discovery. The study on total fungal community associated with marine macroalga U. intestinalis from Xiamen-China was carried out using MiSeq, a powerful platform for microbial ecology studies. Metagenomics DNA was isolated from surfaces of the U. intestinalis thallus and rocks colonized by this alga and  18S ribosomal RNA region was sequenced using MiSeq Illumina to yield 81360 sequences from 162720 reads, which average read length is 245.61 bp. The sequences were assigned to operational taxonomic units (OTUs), where 101 genera represent 5 phyla, 17 classes and 64 families of fungal. Ascomycota was the highest abundance share of fungal community at phylum level. Meanwhile Aspergillus, Blastobotrys, Alternaria, Knufia, and Fusarium were some of dominant genera found associated with U. intestinalis which have been reported as the promising source of natural products. By using MiSeq platform, this study revealed the total community structure of algicolous fungi associated with U. intestinalis.

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