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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 12, No 1 (2017): May 2017" : 8 Documents clear
Back Cover Squalen Bulletin Vol. 12 No. 1 Tahun 2017 Squalen Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.313

Abstract

Determination and Optimization of Flour for Producing Crispy Baby Tilapia using Plackett Burman Design and Central Composite Design Method Syamdidi Syamdidi; Diah Ikasari; Hasta Octavini
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.282

Abstract

Research on processing of crispy baby tilapia (Oreochromis niloticus) was conducted to obtain type and proportion of flour on this product with central composite design method. This research used 6 types of flour, namely wheat flour, rice flour, potato flour, tapioca flour, corn flour and baking powder. Baby tilapia used for this research were 30-40 day old, 2-3 cm long. Parameters observed were sensory (appearance, odor, taste, texture, overall acceptance) and crispness for the physical parameter. The results showed that only two out of six variables gave big effect on the tested response i.e potato and rice flour. Those two variables were then optimized with central composite design method to obtain the best product. The optimization process demonstrated that the optimum amount of potato and rice flour were 58-60 g (22.16-22.92%) and 40-60 g (15.28-22.92%), respectively.
Preface Squalen Bulletin Vol. 12 No. 1 Tahun 2017 Squalen Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.312

Abstract

Cembranoids Content of Soft Coral Sarcophyton from Acidified Environment at Volcano Island, Indonesia Hedi Indra Januar; Neviaty Putri Zamani; Dedi Soedharma; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.276

Abstract

Cembranoid content in soft coral is known as a chemotype that relate with genotype and environment. This research aimed to characterize the cembranoid Sarcophyton soft coral from the reef that acidified by CO2 volcanic vents (pHT 7.8) at Volcano Islands waters, Banda-Neira (Indonesia), as a means of predicting the future impact of ocean acidification to the genetic diversity of Sarcophyton soft coral. 30 random colonies were taken, combined, and extracted with ethanol. Cembranoid isolation and identification had been done by high performance liquid chromatography and spectroscopic techniques. Results of the study found sarcophytol derivatives (sarcophytol A, 11,12-epoxy sarcophytol A, sarcophytol B, and sarcophytol M) as the only chemotype in the sample. This may suggest low genetic diversity in the observed Sarcophyton sample. Therefore, it may suggest that even soft coral is known to be resilient to future acidification pressures, the genetic diversity or the production of diverse cytotoxic metabolite may be hampered due to ocean acidification in future climate change adaptation.
Front Cover Squalen Bulletin Vol. 12 No. 1 Tahun 2017 Squalen Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.311

Abstract

Cloning of Partial β-Mannanase Gene from Indonesia Marine Bacteria Bacillus Subtilis LBF-005 Yopi yopi; Nanik Rahmani; Maghfirotul Amaniyah; Apridah Cameliawati Djohan
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.252

Abstract

 The strain LBF-005 from marine bacteria have already isolated and screened for mannanase degrading enzyme in submerged fermentation process. This strain was further identified by using 16S rRNA showed that bacterium is belong to Bacillus subtilis that could produce mannanase with activity around 9.5 U/mL. The optimum pH and temperature for the activity of crude enzyme for mannanase was 6.0 and 50 oC. Cloning of mannanase gene from B. subtilis was conducted using six primers set designed based on the homology analysis conserve region several mannanase from bacteria (Bacillus sp.) glycosyl hydrolase (GH) family 26. Optimization of PCR conditions was performed by gradient PCR to obtained PCR product of b-mannanase gene. The PCR product was obtained by third primer combination and was estimated to be around 972-bp. Analysis of the nucleotide sequence showed that sequences has similarity with mananase gene from other Bacillus sp., such as the B. subtilis strain WLY-12, B. subtilis strain WL-8, B. subtilis strain CICC 10260, B.subtilis strain CD-25, B.subtilis strain G1, and Bacillus sp. SWU60 were about 98%, 98%, 98%, 98%, 97% and 95%, respectively. The next research will to obtain a whole gene encoding β-mannanase by using in vitro cloning method, characterization of recombinant mannanase and application this enzyme for mannooligosaccharides production.
Metagenomics-Based Cloning of Amilase-Encoding Genes from the Uncultured Symbiotic Bacteria of a Marine Sponge Theonella swinhoei from Kapoposang Island, South Sulawesi Priyono, Franciscus Edi; Zilda, Dewi Seswita; Kusnadi, Yudi; Hadi, Tri A; Nurrachmi, Irvina; Uria, Agustinus Robert
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.272

Abstract

Marine sponges have recently been recognized as the source ofenzymes, including members of hydrolases. Hydrolytic enzymes are extracellularly produced by sponge-associated bacteria to mediate the metabolism of complex organic matters, thereby assisting the sponge hosts in nutrition and metabolic processes. Among hydrolytic enzymes, amilaseshas attracted increasing attention due to their potential industrial applications. This research work was aimed atutilizing functional metagenomicsapproach for the discovery of amilases derived from the uncultured symbiotic bacteriaof the Indonesian marine sponge Theonella swinhoei. Weinitially constructed a small-insert metagenomiclibrary in Escherichia coliby cloning of metagenome in the size range of5-20 kb prepared from the sponge’s microbiome. Further functional screening of the resulting metagenomic library led to the isolation of two recombinant E. coli clones potentially harboring amilase genes, as indicated by the presence of clearing zones surroinding the selective medium containing 1% amilum. 
Optimum Age of Starter for Microbial Transglutaminase (MTGase) Production Produced by Streptomyces thioluteus TTA 02 SDS 14 and Characterization of Crude Enzyme Dewi Seswita Zilda; Yusro Nuri Fawzya; Lia Siti Nur'amaliyah; Hana Nurullita Prestisia; Nisa Rachmania Mubarik; Puspita Lisdiyanti
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.283

Abstract

The condition of starter plays important role in enzyme production. Optimum starter will lead to the optimum yield of enzyme production. The experiments which were aimed to obtain the optimum  age for starter of Streptomyces thioluteus TTA 02 SDS 14  from solid as well as liquid medium had been carried out which would be used for transglutaminase production. Medium used for culture maintenance, starter optimization and enzyme production was described by Bahrim. The result showed that S. thioluteus TTA 02 SDS 14 was ready to be used as starter after being cultured  on solid medium continued in liquid medium each  for 6 days. The enzyme production in a bioreactor using optimized starter produced enzyme with the highest activity after being fermented for 2 days with 150 rpm agitation. The crude enzyme active optimally at 45-50 oC, pH of 6 and  no effect of metal ion and inhibitor on enzyme activity.  

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