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Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
ISSN : 20895690     EISSN : 24069272     DOI : -
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 8 Documents
Search results for , issue "Vol 14, No 3 (2019): December 2019" : 8 Documents clear
Preface Squalen Bulletin Vol. 14 No. 3 Tahun 2019 squalen bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

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Abstract

Back Cover Squalen Bulletin Vol. 14 No. 3 Tahun 2019 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

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Abstract

Potency of Catfish (Clarias sp.) Protein Hydrolysates as Candidates Matrices for Microbiology Reference Material Kurniawati, Eti; Ibrahim, Bustami; Desniar, Desniar
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.542 KB) | DOI: 10.15578/squalen.v14i3.404

Abstract

Fish protein hydrolysate (FPH) is a derivative product of fish proteins containing smaller peptides and amino acids. FPH products have high water solubility, good emulsion capacity, and large expanding ability. With its functional properties, it allows FPH to be used as a raw material in the manufacturing of secondary microbiological reference materials. This study was intended to characterize catfish (Clarias sp.) FPH as a candidate for the matrix of microbial secondary reference. The FPH was prepared through enzymatic hydrolysis, freeze-drying and milling. The hydrolysis processes were carried out using 5% (w/w) papain, 55 °C for 5 hours, then the papain activity was stopped by increasing the temperature to 80 °C for 20 minutes.The FPH was combined with gelatine, sodium glutamate, glucose solution, and was spiked with Salmonella enteritica sv Enteritidis and freeze-dried. Results showed that catfish FPH was yellowish-white powder with a FPH yield of 11.05%. The proximate analysis of FPH revealed the moisture content of 3.77 ± 0.12%, ash content of 7.26 ± 0.03%, protein content of 86.09 ± 0.17%, and fat content of 1.38 ± 0.07%. The protein content of the FPH was greater than skim milk (33.42%). Carbohydrate levels of catfish FPH and skim milk were 1.56% and 57.46%, respectively. The best concentration of catfish FPH to perform as a microbiological reference material was 14%, obtained from highest viability of Salmonella bacteria and homogeny. The candidate for reference material were stable at storage temperatures of -20 oC.
Growth Hormone, Nitrogen and Potassium Content in the Formulated Solid Waste from Agar Processing for Fertilizer Application Jamal Basmal; Muhamad Luthfian Henrida; Rinta Kusumawati; Nurhayati Nurhayati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.43 KB) | DOI: 10.15578/squalen.v14i3.385

Abstract

The solid waste from agar processing industries in Indonesia is enormous. The waste contains numbers of macro and micro nutrient including growth hormone that can be a good source for plant fertilizer. Utilization of solid waste obtained from agar processing into fertilizer has been carried out by mixing with Sargassum powder, fish silage and Eucheuma cottonii pasta. Various concentrations of the solid waste (13, 19, 22 and 25%) and the Sargassum powder (0, 3, 6, 9, and 12%) were used in this study to obtain optimum nutrients  in the formulated fertilizers i.e. growth hormone, total nitrogen and potassium. Growth hormone were analyzed using high-performance liquid chromatography (HPLC), while N content by Kjeldahl methods and K content analyzed according  AOAC.The results showed that the optimum formulation was obtained from the combination of 25% solid waste and 0% Sargassum powder, which contained the highest growth hormones i.e. auxins (IAA) 46.75 ppm, gibberellin (GA3) 22.25 ppm, cytokinin-zeatin 18.50 ppm, and cytokinin-kinetin 61.00 ppm. While the potassium content was 0.43±0.001 ppm and the total nitrogen was 0.53± 0.06%. This result meets the requirements of the Minister of Agriculture Regulation No. 261/KPTS/SR.310/M/4/2019 regarding the requirements for total N in organic fertilizer. However, the total K+ does not meet the requirement. Therefore, enrichment with other organic raw material is still needed.
Front Cover Squalen Bulletin Vol. 14 No. 3 Tahun 2019 squalen bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

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Emerging Pollutants from Industrial Emission and Their Health Hazards in Indonesian Seafood: A Review Dwiyitno Dwiyitno
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i3.399

Abstract

Emerging pollutants (EPs) or contaminants of emerging concern (CECs) have become global awareness since few decades ago, including in Indonesia. Intensive usage of industrial compounds has led to the massive emission to the environment and therefore their potential adverse effects may endanger aquatic organisms and human health. Based on the available literatures, organotins and flame retardants are two main groups of EPs from industrial emission identified in Indonesian seafood, as well as in those worldwide. However, concentration of both EPs group detected in Indonesian seafood is relatively low than that in developed countries and the majority of South East Asian countries. Toxicological studies revealed that EPs emitted from industrial activities have to be concern, as most of the EPs attributed to endocrine disrupting chemicals. Nevertheless, study on the exposure assessment of EPs in Indonesia is very limited. Even though, the concentrations of EPs in Indonesian seafood produce exposure below tolerable daily intake (TDI) to the local consumer, the long term exposures have to be aware due to their possible continues emission and elevating concentration such as shown by flame retardants. Governmental regulation, monitoring programs, and laboratory approach are among issues to be concern in addressing EPs in Indonesia, especially from industrial emission.
Extraction and Partial Characterization of Lectin from Indonesian Brown Algae Padina australis and Padina minor Nurrahmi Dewi Fajarningsih; Naomi Intaqta; Danar Praseptiangga; Choiroel Anam
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i3.400

Abstract

Extraction and partial characterization of lectin from Indonesian Padina australis and Padina minor had been carried out. The crude extract of the P. australis and P. minor were examined for hemagglutination activity (HA) using native and trypsin-treated of rabbit and human A, B, O type erythrocytes. Both extracts agglutinated all of the trypsin-treated erythrocytes tested in the HA assay. Strong HA was detected in the crude extract of P. minor with trypsin-treated of human type A and O erythrocytes. However, the sugar-binding specificity study through the quantitative hemagglutination inhibition (HI) assay showed that P. minor extract could not specifically recognize the glycans tested. Apparently, the HA of the P. minor was more due to its co-extracted polyphenols content than its lectin content. On the other hand, the HI assay showed that asialo transferrin human (aTf) and asialo porcine thyroglobulin (aPTG) were the most powerful in inhibiting the HA of P. australis. Those indicated that P. australis protein extract was able to specifically recognized aTf and aPTG. The stability of P. australis and P. minor HA over various temperatures, pH ranges, and divalent cations studies showed that the P. minor HA was stable on a wide range of pH and temperature; not affected by the presence of EDTA, but decreased by Ca2+ and Mg2+ additions showed that P. minor protein extract  was not a metallic protein. The HA of P. australis decreased at 60 oC and was inactivated at 90 oC; increased at strong acidic (pH 3 4) and strong basic (pH 9 10) and dependent by the presence of either EDTA or Ca2+ and Mg2+ divalent cation.
Increasing Chitinase Activity of Serratia marcescens PT-6 through Optimization of Medium Composition Akhmad Awaludin Agustiar; Imas Faturrohmah; Bekti Wulan Sari; Nurul Binti Isnaini; Indun Dewi Puspita; Triyanto Triyanto; Amir Husni; Ustadi Ustadi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i3.414

Abstract

Chitin hydrolysate is one of the value added product derived from shrimp shell waste. Production of chitin hydrolysate using biological process offers an environmental friendly method compared to chemical process. Serratia marcescens PT-6, a gram negative chitinolytic bacterium isolated from shrimp pond sediment, shows good activity in hydrolyzing chitin. This study aimed to improve the chitinase activity of S. marcescens PT-6 culture by optimizing the component of chitin-containing medium (additional nitrogen source, additional carbon source, and colloidal chitin). The optimization of chitinase by S. marcescens PT-6 culture was done using one variable at a time method. The sequence of the research were to optimize 1) the type of additional carbon source (glucose, lactose, sucrose, and starch), 2) the type of additional nitrogen source (yeast extract, peptone, ammonium sulphate, and ammonium chloride), 3) the concentration of colloidal chitin (0.5; 1; 1.5; 2; and 2.5%), and 4) the concentration of the additional carbon and nitrogen source. The culture of S. marcescens PT-6 was incubated in colloidal chitin medium at 30 oC and chitinase activity from culture supernatant was analyzed. The results showed that starch gave the highest chitinase activity compare to other carbon source, meanwhile yeast extract was chosen as the best nitrogen source among others. The combination of 1.5% colloidal chitin with 0.5% starch and 0.1% yeast extract in medium increased the chitinase activity of S. marcescens PT-6 to 0.021 U/ml. These results indicated that an appropriate medium composition could increase the chitinase activity produced by S. marcescens PT-6 culture.

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