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ILMU KELAUTAN: Indonesian Journal of Marine Sciences
Published by Universitas Diponegoro
ISSN : 08537291     EISSN : 24067598     DOI : -
Core Subject : Science,
ILMU KELAUTAN: Indonesian Journal of Marine Sciences (IJMS) is dedicated to published highest quality of research papers and review on all aspects of marine biology, marine conservation, marine culture, marine geology and oceanography.
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Articles 7 Documents
Search results for , issue "Vol 20, No 1 (2015): Ilmu Kelautan" : 7 Documents clear
Phylogenetic Analysis of the Darkfin Hind, Cephalopholis urodeta (Serranidae) Using Partial Mitochondrial CO1 Gene Sequences (Analisis Filogenetik Cephalopholis urodeta (Serranidae) Menggunakan Runutan Gen CO1 Mitokondria Parsial) Yanti Ariyanti; Achmad Farajallah; Irma Shita Arlyza
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 1 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.879 KB) | DOI: 10.14710/ik.ijms.20.1.38-44

Abstract

Cephalopholis merupakan salah satu genera terbesar dalam subfamili Epinephelinae yang memiliki banyak species. Secara fenotip, C. urodeta dewasa mirip dengan juvenil C. sonnerati karena memiliki ciri mencolok yaitu garis yang menyudut pada sirip ekor. Untuk memahami hubungan genetik pada spesies ikan ini, maka dilakukan analisis molekuler menggunakan ruas gen CO1. Sejumlah spesies ikan (famili Serranidae) dikumpulkan dari wilayah Sulawesi Selatan seperti Sinjai dan Kepulauan Selayar. Karakter fenotip diidentifikasi menggunakan buku katalog spesies kerapu dunia FAO, kemudian sampel yang diduga C. urodeta secara morfologi dipisahkan. Jaringan yang digunakan sebagai sumber DNA adalah jaringan otot bagian dorsal. Berdasarkan sebagian runutan gen CO1, diyakini bahwa sampel tersebut adalah C. urodeta. Runutan basa nukleotida dari sampel dibandingkan dengan 22 runutan basa nukleotida C. urodeta dari GenBank. Berdasarkan rekonstruksi pohon filogeni, C. urodeta dari Sinjai dan Kepulauan Selayar mengelompok dengan C. urodeta dari berbagai tempat seperti Polynesia, Mariana Utara, Filipina, pulau-pulau di sekitar Madagascar (Ouest, St. Gilles, Canyon, Cimetiere, Jaune) dan Adaman, sedangkan sampel dari Laut Arab di lepas pantai India berada pada cabang yang terpisah. Penelitian ini menyatakan bahwa C. urodeta yang melibatkan beberapa tempat dari berbagai perairan seperti Samudera Pasifik bagian Selatan (Polynesia), Samudera Pasifik bagian Utara (Northern Mariana), Laut China Selatan (Filipina), Teluk Bengal (Andaman), Laut Laccadive (reunion of Ouest, St. Gilles and Cimetiere), Laut Arab dan Indo Pasifik Barat (Indonesia) memiliki perbedaan jarak genetik yang kecil. Hal ini berimplikasi pada pemahaman pola migrasi spesies tersebut dan sebagai bahan pertimbangan pengambilan kebijakan konservasi. Kata kunci: Cephalopholis urodeta, CO1, filogenetik, Serranidae, Sulawesi Selatan Cephalopholis is one of the largest genera belonging to Subfamilly Epinephelinae, which has various species. Phenotypically, an adult C. urodeta similar to a juvenile of C. sonnerati, since both of them have a striking trait, two white oblique stripes or bands on the caudal fins. This work was conducted to investigate the genetic relationships of this species using CO1 gene segment. Fish were collected from several sampling point in South Sulawesi areas such as Sinjai and Selayar Island. The phenotypic characterizations were identified using the FAO species catalogue of groupers of the world, and the species that seemed to have C. urodeta morphology then separated. Tissue samples from dorsal muscle tissue were used as the source of DNA. Using part of the CO1 gene sequence, it can be confirmed that our samples are exactly C. urodeta species. The 22 C. urodeta sequences from GeneBank compared with our sequences. Interestingly, because based on the phylogenetic tree, our sequences clustered with the other C. urodeta sequences from several part of the world except the Arabian Sea off the coast of India, which is a separate branch. The present study reveals less genetic distance in C. urodeta than some other parts of the ocean as follows; South Pacific Ocean (Polynesia), North Pacific Ocean (Northern Mariana), South China Sea (Philippines), Andaman, west coast of Réunion Island, Arabian Sea and Indo West Pacific (Indonesia). This has implications for understanding the migration pattern of the species and may affect conservation policy decisions. Keywords: Cephalopholis urodeta, CO1, phylogenetics, Serranidae, South Sulawesi
Isolation of Lactic Acid Bacteria That Produce Protease and Bacteriocin-Like Substance From Mud Crab (Scylla sp.) Digestive Tract (Isolasi Bakteri Asam Laktat yang Menghasilkan Protease dan Senyawa Bacteriocin-Like dari Saluran Pencernaan Kepiting Heru Pramono; Pipin Suciati; Sapto Andriyono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 1 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.947 KB) | DOI: 10.14710/ik.ijms.20.1.33-37

Abstract

Saluran pencernaan merupakan lingkungan kompleks yang terdiri atas berbagai spesies bakteri. Saluran pencernaan biota perairan terdiri atas bakteri aerob dan anaerob yang mampu memproduksi senyawa antibakteri dan enzim. Tujuan penelitian ini adalah untuk mengisolasi bakteri asam laktat yang menghasilkan protease dan senyawa bakteriosin-like dari saluran pencernaan kepiting bakau. Isolasi dan karakterisasi isolat dilakukan menggunakan media MRS. Supernatan netral bebas sel isolat telah diuji dengan menggunakan disc difusi agar terhadap bakteri patogen dan pembusuk. Uji produksi enzim protease telah diuji dengan metode disc diffusion agar menggunakan media kasein agar. Di antara seratus isolat, terdapat 96 isolat menunjukkan zona bening di MRS + CaCO3,, katalase negatif, dan bakteri Gram positif. Tiga puluh empat isolat bakteri asam laktat menghasilkkan protease dan hanya empat isolat (yaitu IKP29, IKP30, IKP52, dan IKP94) menunjukkan penghambatan yang kuat terhadap bakteri patogen dan pembusuk. Terdapat tiga pola inhibisi dari keempat isolate terhadap Bacillus subtilis, Staphylococcus aureus, Eschericia coli, dan Salmonella sp. Empat isolat tersebut berpotensi untuk dimanfaatkan sebagai starter pada produksi fermentasi produk hasil perikanan. Penelitian ini adalah penelitian pertama terkait isolasi bakteri asam laktat yang menghasilkan protease dan bakteriosin dari saluran pencernaan dari kepiting bakau.Kata kunci: Bakteri Asam Laktat, Bakteriosin-like substance, Protease, Scylla  sp. Digestive tract is complex environment consist of large amount of bacteria’s species. Fish intestine bacteria consist of aerobic or facultative anaerob bacteria which can produce antibacterial and enzym. The objectives of this research were to isolated lactic acid bacteria that produce bacteriocin-like and protease from mud crab digestive tract. Isolation and characterization of isolates were conducted employing media MRS.  Neutralized cell free supernatant of isolates were tested using disc diffusion agar of against pathogenic and spoilage bacteria to indicate bacteriocin-like-producing lactic acid bacteria. Protease-producing isolate was tested using disc diffusion method in casein agar. Among a hundred isolates, 96 isolates were showed clear zone in MRS+CaCO3,, catalase negative, and Gram positive bacteria. Thirty four isolates produced protease and only four isolates (i.e. IKP29, IKP30, IKP52, and IKP94) showed strong inhibition against pathogenic and spoilage bacteria. There were three patterns of inhibition among three isolates against Bacillus subtilis, Staphylococcus aureus, Eschericia coli, and Salmonella sp. All three isolates showed potential uses for produce starter culture for fishery product fermentation purpose. This is the first report of isolation lactic acid bacteria that produced protease and bacteriocin-like from digestive tract of mud crab. Keywords: Lactic acid bacteria, Bacteriocin-like substance, Protease, Scylla  sp.
Wave Transformation for International Hub Port Planning (Transformasi Gelombang untuk Perencanaan Pelabuhan Hub Internasional) Denny Nugroho Sugianto; Purwanto Purwanto; Andika B Candra
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 1 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1549.712 KB) | DOI: 10.14710/ik.ijms.20.1.9-22

Abstract

Indonesia merupakan salah satu negara kepulauan terbesar di dunia sehingga peran pelabuhan sangat vital dalam pembangunan ekonomi. Pelabuhan bukan hanya sekedar sebagai pelengkap infrastruktur, melainkan harus direncanakan dan dikelola dengan baik serta memperhatikan fenomena dinamika perairan laut seperti pola gelombang laut. Data gelombang laut menjadi faktor penting dalam perencanaan tata letak dan tipe bangunan pantai karena dipengaruhi oleh tinggi gelombang signifikan, tunggang pasang surut dan transformasi gelombang. Penelitian ini mengalisis karaketristrik dan bentuk transformasi gelombang untuk perencanaan Pelabuhan Hub Internasional, sebagai studi kasus adalah pelabuhan di Kuala Tanjung, Kabupaten Batu Bara. Pelabuhan di Kuala Tanjung merupakan salah satu dari 2 pelabuhan hub internasional yang direncanakan akan dibangun oleh pemerintah Indonesia. Metode yang digunakan adalah metode kuantitatif yang dilakukan dengan perhitungan statistik dan pemodelan matematik dengan modul hydrodinamic dan spectral wave untuk mengetahui arah penjalaran dan transformasi gelombang. Hasil dari data ECMWF selama 1999–Juni 2014, diketahui tinggi gelombang signifikan (Hs) maksimum mencapai 1,69 m dan periode maksimum 8 detik. Karakteristik gelombang termasuk klasifikasi gelombang laut transisi dengan nilai d.L-1 berkisar anrata 0,27–0,48 dan berdasarkan periodenya diklasifikasikan sebagai gelombang gravitasi.Transformasi gelombang terjadi akibat pendangkalan dengan koefesian pendangkalan Ks 0,93–0,98 dan proses refraksi gelombang dengan koefesien Kr 0,97–0,99. Tinggi gelombang pecah Hb sebesar 1,24 meter dengan kedalaman gelombang pecah db sebesar 1,82 meter. Efektifitas desain bangunan terminal di Pelabuhan Kuala Tanjung secara keseluruhan untuk sepanjang musim sebesar 79,8% atau dapat dikatakan cukup efektif dalam meredam gelombang. Kata kunci: transformasi gelombang, tinggi dan periode gelombang, pelabuhan Indonesia is one of the largest archipelagic countries in the world, therefore port has vital role in economic development. Port is not just as a complement to the infrastructure, but it must be planned and managed properly and attention to the dynamics of marine phenomena such as ocean wave patterns. Ocean wave data become important factors in planning coastal building, since it is influenced by wave height, tides and waves transformation. The purpose of this study was to analyse characteristic and forms wave transformations for planning of international hub port at Kuala Tanjung, Baru Bara District North Sumatra. This port is one of two Indonesian government's plan in the development of international hub port. Quantitative method was used in this study by statistical calculations and mathematical modeling with hydrodinamic modules and spectral wave to determine the direction of wave propagation and transformation. Results show that based on ECMWF data during 1999-June 2014, known significant wave height (Hs) maximum of 1.69 m and maximum period (Ts) of 8 secs. The classification wave characteristics iswave transition (d.L-1: 0.27–0.48) and by the period are classified as gravitational waves. Wave transformation occurs due to the soaling, withKs 0.93–0.98 and the wave refraction Kr 0.97–0.99. Whereas Hb of 1.24 meters anddb 1.82 meters. The effectiveness of the design of the terminal building at the Port of Kuala Tanjung overall for the season amounted to 79.8%, which is quite effective in reducing the wave. Keywords: wave transformation, wave height and period, Port of Kuala Tanjung
Prevalence, Insidence and Progression Black-band Disease on Scleractinian Coral (Montipora spp) in Shallow Water of Pari Islands Michael Delpopi; Neviaty Putri Zamani; Dedi Soedarma; Ofrie Johan
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 1 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.103 KB) | DOI: 10.14710/ik.ijms.20.1.52-60

Abstract

Black-band disease (BBD) is a persistent disease that cause the decline of the coral reef ecosystems, which is still slightly recorded in Indonesia, including in Pari Island, Thousand Islands. The objectives of this study were to determine progression, prevalence and incidence BBD on Montipora spp in shallow water of Pari Islands. Coral cover were estimated using line intercept transect (LIT) whereas the prevalence and incidence of BBD were carried out by using belt transect method with 1 m left and right of tape as long 20 m with 3 replications recorded approximately every two months. BBD progression was documented with a digital photograph method, the photograph of affected area of each coral was taken each day for 2 weeks. BBD was found to be spread in the surveyed area. The result show that prevalence and incidence of BBD showed a positive exponential relationship with water temperature. During the observation Both prevalence and incidence increased on May transitional season (wet-dry). The highest prevalence was found at 5,96 percent and whereas the maximum disease progression found at 0,46 cm/day.
Potential Marine Fungi Hypocreaceae sp. as Agarase Enzyme to Hydrolyze Macroalgae Gelidium latifolium (Potensi Jamur Hypocreaceae sp. sebagai Enzim Agarase untuk menghidrolisis Makroalga Gelidium latifolium) Mujizat Kawaroe; Dwi Setyaningsih; Bertoka Fajar SP Negara; Dina Augustine
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 1 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.08 KB) | DOI: 10.14710/ik.ijms.20.1.45-51

Abstract

Agarase dapat mendegradasi agar ke oligosakarida dan memiliki banyak manfaat untuk makanan, kosmetik, dan lain-lain. Banyak spesies pendegradasi agar adalah organismelaut. Beberapa agarase telah diisolasi dari genera yang berbeda dari mikroorganisme yang ditemukan di air dan sedimen laut. Hypocreaceae sp. diisolasi dari air laut Pulau Pari, Kepulauan Seribu, Jakarta, Indonesia. Berdasarkan hasil identifikasi gen 16S rDNA dari 500 basis pasangan, isolat A10 memiliki 99% kesamaan dengan Hypocreaceae sp. Enzim agarase ekstraseluler dari Hypocreaceae sp. memiliki pH dan suhu optimum pada 8 TrisHCl (0,148 μ.mL-1) dan 50°C (0,182 μ.mL-1), masing-masing. Enzim Agarase dari Hypocreaceae sp. mencapai kondisi optimum pada aktivitas enzim tertinggi selama inkubasi dalam 24 jam (0,323 μ.mL-1). SDS page mengungkapkan bahwa ada dua band dari protein yang dihasilkan oleh agarase dari Hypocreaceae sp. yang berada di berat molekul 39 kDa dan 44 kDa dan hidrolisis Gelidium latifolium diperoleh 0,88% etanol. Kata kunci: enzim agarase, Hypocreaceae sp., hidrolisis, fungi, rDNA. Agarase can degradedagarto oligosaccharide and has a lot of benefits for food, cosmetics, and others. Many species of agar- degrader are marine-organism. Several agarases have been isolated from different genera of microorganisms found in seawater and marine sediments. Hypocreaceae sp. was isolated from sea water of Pari Islands, Seribu Islands, Jakarta, Indonesia. Based on the results of the 16S rDNA gene identification of 500 base pairs, A10 isolates had 99 % similarity toHypocreaceae sp. The extracellular agarase enzyme from Hypocreaceae sp. have optimum pH and temperature at 8 TrisHCl (0.148 µ.mL-1) and 50 °C (0.182 µ.mL-1), respectively. Agarase enzyme of Hypocreaceae sp. reach an optimum condition at the highest enzyme activity during incubation in 24 hours (0.323 µ.mL-1). SDS Page revealed that there are two bands of protein produced by agarase of Hypocreaceae sp. which are at molecular weight of 39 kDa and 44 kDa and hydrolisis of Gelidium latifolium obtained 0,88% ethanol. Key words: agarase enzym, Hypocreaceae sp., hydrolysis, marine fungi, rDNA 
Simulating Wind Driven Waves in the Strait of Hormuz using MIKE21 (Simulasi Gelombang Angin di Selat Hormuz Menggunakan MIKE21) Faeghe Eslami Mehdiabadi; Mehdi Mohammad Mehdizadeh; Maryam Rahbani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 1 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (987.775 KB) | DOI: 10.14710/ik.ijms.20.1.1-8

Abstract

Daerah pesisir di bumi adalah salah satu zona paling dinamis yang dipengaruhi oleh berbagai parameter seperti gelombang, arus, dan badai. Untuk mengelola serta mengkontrol zona tersebut adalah penting untuk mempelajari hidrodinamika daerah. Penelitian ini menggunakan MIKE 21/3 coupled Model FM untuk melihat gelombang (wind driven waves) di sekitar Pulau Larak di Selat Hormuz. Untuk mensimulasikan pola gelombang di wilayah tersebut digunakan irregular triangular grid.  Pola arus di sekitar pulau dipelajari untuk jangka waktu satu tahun. Ditemukan bahwa gelombang yang ditimbulkan karena arus di sekitar pantai utara pulau relatif lemah. Terlihat pula bahwa gelombang yang ada di daerah tersebut terutama ke arah timur laut. Tinggi gelombang rata-rata di surfzone adalah sekitar 0,5 m., Dengan kecepatan arus sekitar 0,2 m.s-1. Mengingat angin menang dan arah gelombang, disimpulkan bahwa pantai utara Pulau Larak tempat yang cocok untuk konstruksi pelabuhan dan kegiatan memancing. Kata kunci: gelombang, arus, MIKE, Pulau Larak Coastal areas on earth are among the most dynamic zones which affected by different parameters such as waves, currents, and storms. To manage and control such a zone it is essential to study the hydrodynamic of the area. MIKE 21/3 Coupled Model FM was used to investigate the wind driven waves around Larak Island located in the Strait of Hormuz. To simulate the pattern of the wave in the area irregular triangular grid was applied. The pattern of current around the Island was studied for a one year period of simulation. It was found that the current induced wave break around the Northern coast of the Island is relatively weak. It was also observed that the prevailed wave in the area is mainly toward the Northeast. The averaged wave height in the surfzone is about 0.5 m., with the current velocity of about 0.2 m.s-1. Considering the prevailed wind and wave direction, it was concluded that the northern coasts of the Larak Island are suitable places for harbor construction, recreational and fishing activities. Keywords: Waves Break, Current, MIKE, Larak Island 
Growth of Favia and Favites Coral Transplants Based on Polyps Number (Pertumbuhan Karang Transplan Genus Favia dan Favites Berdasarkan Jumlah Polip) Diah Permata Wijayanti; Elis Indrayanti; Wandi Febrian Asri; Ambariyanto Ambariyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 1 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (643.85 KB) | DOI: 10.14710/ik.ijms.20.1.23-32

Abstract

Budidaya karang melalui teknik transplantasi merupakan salah satu cara untuk menyelamatkan keberadaan karang terumbu di alam. Selain untuk diperdagangkan, karang hasil transplantasi juga dapat ditanam untuk memperbaiki terumbu karang yang rusak. Penelitian ini menggunakan ukuran dan bentuk polip Favia dan Favites sebagai dasar penentuan ukuran benih untuk mengurangi kerusakan koloni donor. Penelitian bertujuan untuk mengetahui pertumbuhan transplan Favia dan Favites. Penelitian dilakukan secara eksperimental dengan rancangan acak lengkap. Dua ukuran polip dipilih mewakili ukuran benih kecil dan besar, yaitu 2 cm dan 4 cm. Penanaman karang transplan dilakukan di Teluk Awur selama bulan April hingga November. Pengamatan dilakukan tiap 2 minggu sekali. Analisa data menggunakan ANCOVA. Analisa statistik menunjukkan pertumbuhan kedua ukuran transplan tidak berbeda nyata (P>0,05). Pertumbuhan transplan Favia dan Favites ditandai dengan munculnya tunas intratentakular. Pertumbuhan transplan tertinggi dicapai transplan Favites ukuran 4 cm dengan kecepatan tumbuh 0.596 cm2.bulan-1, sedangkan pertumbuhan terendah ditunjukkan oleh transplan Favites ukuran 2 cm dengan pertumbuhan 0.463 cm2.bulan-1. Kata kunci: pertumbuhan karang, transplantasi, Favia, Favites, jumlah polip Cultivation coral by using transplantation method is one attempt to alleviatecorals collection in nature. In addition to be traded, the corals can be transplanted to repair damaged coral reefs. This study utilized size and polyps form of coral Favia and Favites to be transplanted to minimize damage of coral donors. The purpose of this study was to investigate the growth of Favia and Favites transplants. The research was carried out by experimental method using a completely randomized design. Two different size of polyps i.e 2 polypsrepresented small size and 4 polyps represented large size were applied for each coral species. Research was conducted from April–November at Teluk Awur Bay, Jepara. The samples were observed every two weeks. Collected data wereanalyzed by repeated measurement ANCOVA. Statistical analysis showed that the growth of transplants did not show significant differences (P>0.05) between each genus and between 2 and 4 polyps. The growth of transplants have shown by extratentacular budding on Favia and intratentacular budding on Favites. The highest growth rate of transplants was shown by 4 polyps of Favites at 0.596 cm2.mo-1 and the lowest by 2 polyps of Favites at 0.463 cm2.mo-1,respectively. Keywords: Coral Growth, Transplantation, Favia, Favites, Number of Polyps 

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