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Bioma : Berkala Ilmiah Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 25982370     DOI : -
BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.
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Articles 262 Documents
Karakterisasi Senyawa Flavonoid Ekstrak Polar Daun Gamal Kultivar Lampung Utara Dan Uji Aktivitasnya Terhadap Kutu Putih Kakao (Planococcus minor, Hemiptera: Pseudococcidae) Nukmal, Nismah; Pasutri, Agata Yelin; Pratami, Gina Dania
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.705 KB) | DOI: 10.14710/bioma.21.1.25-34

Abstract

Cocoa is one of the export commodities that have great prospects. The quality of cocoa in Indonesia are not satisfactory, this is caused by cocoa mealybug (Planococcus minor). Gamal (Gliricidia maculata) contains flavonoid compounds which can be used as bioinsecticides. The aims of research to determine the characteristic of flavonoid compounds in gamal leaf powder which is effective for kill of cocoa mealybug P. minor. Extraction and spectroscopy analysis (UV-Vis and FTIR) were done in the Integrated Laboratory of Technology Innovation Center (ILTIC) and the bioassay was done at Zoology Laboratory of Unila. The results shows that the crude water extract of gamal leaf powder North Lampung Cultivar (NLC) more effective killed lower than purified water extract. It was indicated by value of LC50.72hours the crude water extract than purified water extract (0.11%: 0.27%). The flavonoid compounds of gamal leaf powder NLC have the characteristics of blue fluorescence in UV light λ 254 and 366 nm. The flavonoid with the maximum peak of wavelength 310 could be grouped to flavonone with structural frame O-H, C=O carbonyl, aromatic C=C, and C-O.
Perilaku Vektor Malaria Anopheles farauti Laveran (Diptera: Culicidae) Di Ekosistem Pantai (Kabupaten Biak Numfor) Dan Ekosistem Rawa (Kabupaten Asmat) Propinsi Papua Kawulur, Hanna; Soesilohadi, RC Hidayat; Hadisusanto, Suwarno; Trisyono, Andi
Bioma : Berkala Ilmiah Biologi Vol. 17, No.1, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.599 KB) | DOI: 10.14710/bioma.17.1.34-40

Abstract

Biak Numfor and Asmat districts reported as malaria endemic areas in Papua (Papua Global Fund, 2011). Anopheles farauti mosquito is one of the malaria vectors in the region. Malaria control efforts have been made but there are still many cases of malaria. Malaria control will provide maximum results if there is a match between the vector behavior and programs undertaken. The purpose of this study was to determine the bionomics factors (behavioral) of malaria vector An. farauti mosquitoes in coastal ecosystems (Biak Numfor) and swamp (Asmat); namely: (a). density (b) age and (c) blood-seeking behavior. The method used in this study is human landing collection, resting collection and ELISA blood-feed. The results showed that the population density of An. farauti mosquito did not have a positive relationship with the number of malaria patients in both study areas. The activity of An. farauti mosquito on blood-seeking at the coastal and swamp ecosystems lasted all night but mainly at 18:00 to 19:00 hour. The study also showed that blood-seeking activity mostly done outside of the house. Age estimation of An. farauti mosquito population at coastal ecosystems ranging from 16-18 days, while in the swamp ecosystem is 12-14 days. Population density, approximately age and blood-seeking behavior indicates that An. farauti mosquitoes on the coastal and swamp ecosystems have the potential to be an effective malaria vector.   Keywords: Malaria, Vector, Behavior
Pelacakan Gen Sitokrom Oksidase Subunit 1 (Co1) DNA Mitokondria Pada Itik Tegal (Anas sp.) Rahayu, Annisa Rizky; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.763 KB) | DOI: 10.14710/bioma.18.2.114-122

Abstract

Itik Tegal adalah salah satu sumber plasma nutfah ternak Indonesia yang belum memiliki informasi asal usul dan identitas genetik, sedangkan ini sangat diperlukan sebagai dasar dalam usaha persilangan dan pemuliaan untuk menghasilkan itik hibrida unggul. Penelitian ini bertujuan untuk mengetahui identitas genetik itik Tegal menggunakan gen sitokrom oksidase subunit 1 (CO1) dengan primer BirdF1 dan BirdR1. Penelitian dilakukan dengan cara isolasi DNA dari otot paha itik, diikuti amplifikasi gen CO1, dan sekuensing. Sekuen gen CO1 digunakan untuk analisis hubungan kekerabatan dengan mengkonstruksi pohon filogenetik menggunakan metode neighbor-joining dengan analisis bootstrap 1.000 ulangan. Model Kimura 2-parameter digunakan untuk menghitung jarak genetik dengan pairwise distance. Hasil penelitian memperoleh fragmen gen CO1 itik Tegal. Fragmen tersebut homolog dengan sekuen gen CO1 Anas platyrhynchos voucher NHMO-BC400. Analisis filogenetik menunjukkan itik Tegal memiliki hubungan kekerabatan paling dekat dengan A. platyrhynchos yang terdistribusi di Skandinavia dan Amerika Utara, A. poecilorhyncha yang terdistribusi di Asia tropis dan timur, serta Tadorna tadorna yang terdistribusi di China. Kata kunci: gen CO1, genetik, itik Tegal
Pengaruh Dosis Kompos dengan Stimulator Trichoderma terhadap Pertumbuhan dan Produksi Tanaman Jagung (zea mays l.) Varietas Pioner -11 pada Lahan Kering Afitin, Retno -; Darmanti, Sri -
Bioma : Berkala Ilmiah Biologi Vol. 11, No. 2, Tahun 2009
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.765 KB) | DOI: 10.14710/bioma.11.2.69-75

Abstract

Lahan kering di Indonesia sangat luas. Sifat lahan kering yang kurang subur dengan tingkat erosi yang tinggidan rendahnya pasokan air menyebabkan pemanfaatan lahan kering belum optimal. Kompos sebagai salah satupupuk organik dengan bahan baku yang tersedia cukup banyak merupakan alternatif yang tepat untuk digunakandalam memanfaatkan lahan kering sebagai lahan pertanian. Penelitian menggunakan Rancangan Acak Lengkapdengan 4 perlakuan berupa dosis pemupukan , yaitu : D0 : 0 ton / Ha (kontrol), D1 : 15 ton / Ha, D2 : 30 ton / Hadan D3 : 45 ton / Ha. Data yang diperoleh dianalisis dengan Analisis of Varians (Anava), bila terdapat beda nyatadilanjutkan dengan uji Beda Nyata terkecil (BNT) pada taraf kepercayaan 95%. Dari hasil penelitian dapatdisimpulkan bahwa kompos dengan stimulator tricoderma berpengaruh meningkatkan pertumbuhan dan produksitanaman jagung pada lahan kering dan dosis 3 kg / m2 merupakan dosis optimal untuk pertumbuhan dan produksijagung.
Induksi Kalus Binahong (Basella rubra L.) Secara In Vitro Pada Media Murashige & Skoog Dengan Konsentrasi Sukrosa Yang Berbeda Sitorus, Ertina Novaria; Hastuti, Endah Dwi; Setiari, Nintya -
Bioma : Berkala Ilmiah Biologi Vol. 13, No. 1, Tahun 2011
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2561.529 KB) | DOI: 10.14710/bioma.13.1.1-7

Abstract

Binahong (Basella rubra L.) is a plant medicine consisting secondary metabolites which have virtue asmedicines for several diseases that could also be used as coloring agent. The medicine compounds in secondarymetabolites could be extracted from callus. Sucrose is one of the components that build MS (Murashige & Skoog)medium. Sucrose is important in in vitro culture, it functions as carbon and energy source for explant to grow. Thepurposes of this research are to study the effect of sucrose in MS medium towards B. rubra L callus formation andgrowth; to find the optimum sucrose concentration for callus B. rubra L formation; and also to find the fastestinitiation time to produce callus crumb. This research uses Complete Random Design (CRD) single factor method,i.e., sucrose concentration of 0 g/l, 10g/l, 20 g/l, 30 g/l, 40 g/l with five repetitions. The data is analysed withAnalysis of Varian (Anova) and if a real difference is found the analysis is continued with Duncan Multiple RangeTest (DMRT) with significancy level of 95%. The results show that various sucrose concentrations in MS mediuminfluences callus B. rubra L induction. The highest sucrose concentration, i.e. 40 g/l, which was added into MSmedium, could induce the maximum callus wet-weight of 1,69 g and the fastest callus initiation time of 4,8 day.
Penambahan Filtrat Tepung Umbi Dahlia (Dahlia variabilis Willd.) sebagai Prebiotik dalam Pembuatan Yoghurt Sinbiotik Alfaridhi, Khabib Khasan; Lunggani, Arina Tri; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 15, No.2, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.421 KB) | DOI: 10.14710/bioma.15.2.64-72

Abstract

Yoghurt is a fermented milk product that can provide beneficial effects to the health of consumers. Increasing the quality of yoghurt can be made by combining the benefits of probiotic with prebiotic to produce sinbiotic yoghurt. Inulin is a prebiotic that found in many dahlia tuber (Dahlia variabilis Willd.). Pure inulin were expensive, so the alternative is using dahlia tuber flours filtrate as prebiotic ingredients. This study aimed to examine the addition of Dahlia Tuber Flours Filtrate (DTFF) as a prebiotic in sinbiotic yoghurt making using Lactic Acid Bacteria (LAB) Lactobacillus bulgaricus, Streptococcus termophilus dan Lactobacillus acidopilus as probiotic. This research using Completely Randomized Design (CRD) with 4 concentration treatments DTFF 0%, 3%, 6% and 9% with 3 replications. Observed variables consist of the number of total LAB, lactic acid level, pH and organoleptic attributes (aroma, flavor, consistency and impression). Data the number of total LAB, lactic acid level and pH values ​​were analyzed by ANOVA, continued by Duncan test, while the organoleptic data was analyzed by Mann-Whitney U test with 95% significant level. The results showed that the addition of DTFF significantly affect the increasing of total LAB, lactic acid level, organoleptic assessment and decreasing pH of sinbiotic yoghurt. Sinbiotic yoghurt with 3% of DTFF concentration is most preferred by panelists and required the criteria of Indonesian National Standard (SNI) for yoghurt with enough likely aroma, sour taste/specify and enough thick texture with total LAB 2,4 x 108 CFU/ml, 1.3% lactic acid levels and 4.2. pH. Keywords : prebiotic, probiotic, inulin, dahlia tuber, sinbiotic yoghurt, lactobacillus bulgaricus, streptococcus termophilus, lactobacillus acidopilus
Studi Populasi dan Habitat Owa Jawa (Hylobates moloch Audebert 1797) di Kawasan Wana Wisata Kali Paingan Linggo Asri, Pekalongan, Jawa Tengah Putra, M. Fadhil Randa; Baskoro, Karyadi; Hadi, Mochamad
Bioma : Berkala Ilmiah Biologi Vol. 20, No 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.323 KB) | DOI: 10.14710/bioma.20.2.154-164

Abstract

Javan Gibbon (Hylobates moloch) is an Indonesian endemic primate found on Java, in the province of West Java and Central Java. It is an Endangered species by IUCN and protected by Ministry of Environment and Forestry regulation No. P20/2018. Wana Wisata Kali Paingan was known as Javan gibbon habitat in Central Java, although not much research have been done. This study aimed to find out the population data and habitat of Javan Gibbon. This study was conducted in Wana Wisata Kali Paingan during April until May 2018 and Line Transect method was used in two tracks, Kali Wadas and Jurang Jero, with 1 kilometer and 5 repetition each. Result showed that 12 individuals in 4 groups was found with the density was 13,75 individuals /km2 and 4,39 groups/km2. Then at 6,7 km2 representative area the estimation was obtained 92 individuals and 29 groups. Javan gibbons were distributed from 630-780 MASL with the different sizes of groups. There were 25 species in 21 families trees and 4 species in 4 family poles was found. The dominant species was bamboo (Bambusa vulgaris) and terap tree (Artocarpus elasticus). Javan gibbon’s favorite food trees were Terap tree, Ficus (Urostigma glaberrium) and (Ficus sinuata). There were several families of food trees for Javan gibbons including Moraceae, Euphobiaceae, Meliaceae, Lauraceae, and Fagaceae..   Keywords : Javan gibbon, Population, Habitat, Wana Wisata Kali Paingan
Kemampuan Tumbuhan Typha Angustifolia Dalam Sistem Subsurface Flow Constructed Wetland Untuk Pengolahan Limbah Cair Industri Kerupuk (Studi Kasus Limbah Cair Sentra Industri Kerupuk Desa Kenanga Kecamatan Sindang Kabupaten Indramayu Jawa Barat) Abdulgani, Hamdani; Izzati, Munifatul; S, Sudarno
Bioma : Berkala Ilmiah Biologi Vol. 16, No.2, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.136 KB) | DOI: 10.14710/bioma.16.2.90-101

Abstract

Industrial crackers centers at Kenanga Village has been able to lift the local economy,but that has been polluting the surrounding surface water contamination caused by wastewater discharged directly without any treatment in advance so that the water is black and smells foul . Therefore, the need of alternative wastewater treatment and in the study conducted by the artificial wetlands (constructed wetland) system Sub - surface Flow vertical flow with Typha angustifolia plant and use a medium sized sand 1 mm - 5 mm were previously washed first with media thickness of 30 cm and then operated intermittently using a peristaltic pump 6 times a day with the design of constructed wetlands construction made of wood coated with plastic and reactor dimensions 90 cm x 45 cm x 50 cm . As for the control (no treatment) using use the bucket size diameter 40 cm and height of 20 cm. This study aims to determine the ability of Subsurface Flow Constructed Wetland with Typha angustifolia plants in lowering the concentration of TSS , BOD5 , COD , ammonia (NH3 – N) and sulfide (H2S) at 5,10 and 15 days of processing . The results showed a decrease in the concentration efficiency at consecutive time 5 , 10 and 15 days in Subsurface Flow Constructed wetland with Typha angustifolia for TSS 73,78%; 77,18%; 84,71%; BOD5 85,83%; 90,33%; 94,17%; COD 86,94%; 90,65%; 94,87%; Ammonia 76,07%; 84,25%; 87,52%; sulfide 94,56%; 99,18%; 99,81 %.
Analisis Derajat Ploidi dan Pengaruhnya Terhadap Variasi Ukuran Stomata dan Spora pada Adiantum raddianum Perwati, Lilih Khotim
Bioma : Berkala Ilmiah Biologi Vol. 11, No. 2, Tahun 2009
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.826 KB) | DOI: 10.14710/bioma.11.2.39-44

Abstract

Polyploidy is a common phenomenon in plants, especially on the species of ferns. The objectives of thisresearch are to find out the variation of the ploidy levels, to know the effects on the stomata index and size ofstomata and spore in Adiantum raddianum. The materials used to conduct this observation of chromosomes numberwere made from root tips or young leaf tips (croziers). The modified squash method was used in microscopicpreparation. The result of the observation showed that there was a variation in ploidy levels from 2n = 2x (diploid) to2n = 7x (septaploid). It appeared to be general trend that the higher level the ploidy causes the lower the indexstomata but the bigger the stomata and the spore size.
Perbandingan Struktur Komunitas Mikroartropoda Tanah di Lahan Zona Aktif dan Pasif TPA Jatibarang Semarang Amelinda, Elda Dheiva; Rahadian, Rully; Hadi, Mochamad
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (77.927 KB) | DOI: 10.14710/bioma.19.2.141-149

Abstract

Microarthropods is one of the important components of soil biology which is abundant and varied. The purpose of this research is to compare the structure of microartropod soil community and to examine the influence of physical-chemical factors of the environment based on the dynamics of abundance of soil microartropods in the active zone and the passive zone of Jatibarang Landfill Landfill Semarang. This research was conducted at Jatibarang Landfill Zone of active zone and passive zone by using purposive sampling method. The results showed that the number of species and the number of microartropods in the active zone was higher than the passive zone. In the active zone found 2240 individual / m2 soil microartropods consisting of 21 species and 17 families, whereas in the passive zone found 480 individual / m2 soil microartropods consisting of 10 species and 9 families. The dominating families in both the active and the passive zones are Sciaridae and Cicadellidae. Environmental factors that affect the diversity of soil microartropods are ground water content, soil porosity, heavy metal content such as Pb, Cu, and Zn; As well as the availability of organic matter in the soil. Keyword: Community structure, soil microarthropods, heavy metals, Jatibarang landfill

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