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INDONESIA
Majalah Patologi Indonesia
ISSN : -     EISSN : 25279106     DOI : -
Core Subject : Health, Science,
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Articles 8 Documents
Search results for , issue "Vol 23 No 2 (2014): MPI" : 8 Documents clear
Penurunan Aktivitas Proliferasi Sel Adenokarsinoma Mamma Mencit C3H akibat Pemberian Ekstrak Etanol Lengkuas (Alpinia galanga) Susanto Winarko; Yanwirasti .; Aswiyanti Asri; Salmiah Agus
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Background WHO data in 2011 showed that deaths from breast cancer in Indonesia reached 20,052 peoples of all deaths. This is partly due to the high cost of treatment, so that many people are turning to alternative therapies such as traditional plants. Lengkuas (Alpinia galanga (L) Wild is the traditional material that is cheap and easily available in Indonesia. Lengkuas-containing active substances I-acetoxychavicol acetate (ACA), and according to past research has anti-cancer properties by inhibiting the activity through the inhibition of cell proliferation pathways nuclear factor kappa B (NFƙB). To investigate the effect of ethanol extract of lengkuas against mammary adenocarcinoma cell prolifferation activities in C3H mice with various dose levels. Methods This study was an experimental study using C3H mice with post-test only research design controlled group design. Experimental animals consisted of 32 C3H mice were inoculated with tumor cells and then divided into 4 groups: control group (K) and three treatment groups (P1, P2 and P3) were given graded doses of ethanol extract of lengkuas 225 mg/kgBW/day, 450 mg/kgBW/day and 675 mg/kgBW/ day for 2 weeks. After all mice were terminated, examined the proliferative activity of tumor cells with Ki-67 antibody. Differences in proliferative activity between the groups were tested using one-way anova followed by post hoc Bonferroni test. Results There were significant differences in proliferative activities (p=0.000) among the four groups. By the post hoc Bonferroni analysis was found the significant differences in proliferative activities between the control group P1, with P2 controls, P3 control group, between P1 with P3 group, P2 with P3 group (p=
Korelasi antara Imunoekspresi LMP-1 Virus Epstein-Barr dengan Respon Kemoterapi CHOP pada Limfoma Maligna Non-Hodgkin Tipe Diffuse Large B Cell. Inas Susanti; Hasrayati Agustina; Bethy S Hernowo
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Background Diffuse large B cell lymphoma is the most common type of B-cell non-Hodgkin malignant lymphoma. Epstein-Barr virus (EBV) has been shown to contribute to the development of this tumor especially in the elderly patient (above 50 years). The chemotherapeutic agents CHOP (Cyclophospamide, Hydroxydaunorubicin, Oncovin, Prednison) are standard treatment for non-Hodgkin lymphoma patients, but approximately 30% cases shows non-respone with standard chemotherapy and undergoes progressive disease until death. There are no accurate predictor factors in predicting CHOP chemotherapy respone in patients with DLBCL. The aim of this study is to determine the role of LMP-1 in predicting CHOP chemotherapy respone in patients with DLBCL. Methods This study was done retrospectively in 34 cases of DLBCL. The samples were obtained from the Anatomic Pathology Department of Dr. Hasan Sadikin Hospital from January 2009 to Desember 2011. All cases were reviewed for histopathological diagnoses based on the WHO Classification of 2001 and the specimens were stained with specific antibodies against CD 20 (for diagnoses DLBCL) and LMP-1. The data were analized using rank Spearman’s correlation test. Results This study showed that 31 cases (91.18%) were LMP-1 positive, only 3 cases (8.82%) were negative. Three cases of negative LMP-1 had a good respone to chemotherapy, whereas among 31 positive cases LMP-1 showed good respone to chemotherapy in 21 cases and the remaining 10 cases had a poor respone to chemotherapy. There was no correlation between EBV-LMP-1 immunoexpression with CHOP chemotherapy respone in DLBCL (p=0.436). Conclusion There was no correlation between EBV-LMP-1 immunoexpression with CHOP chemotherapy respone in DLBCL. Key words: CHOP, DLBCL, EBV, LMP-1, non-Hodgkin lymphoma.
Analisis Gambaran Histopatologi Gastritis Kronik dengan dan Tanpa Bakteri Helicobacter pylori Menurut Sistem Sydney Deassy Ariefiany; Abdul Hadi Hassan; Birgitta M Dewayani; Anglita Yantisetiasti
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Background Histopathology definition of chronic gastritis is a chronic inflammation of the gastric mucosa that can cause glandular atrophy and intestinal metaplasia of the gastric mucosal epithelium. Chronic gastritis can be caused by many factors. The most common etiology chronic gastritis by the bacterium Helicobacter pylori (Hp). The incidence chronic gastritis causes of Hp is more common in developing country than developed country. Histopathological picture on chronic gastritis causes of Hp similar with chronic gastritis non-Hp. The aims of this study to determine the difference of histopathological picture between chronic gastritis with Hp and chronic gastritis without Hp bacteria in the Hospital Dr Hasan Sadikin Bandung. Methods The sample used in this study were 30 cases of chronic gastritis with Helicobacter pylori (Hp) and 30 cases of chronic gastritis non-Hp bacteria from the Pathology Department of the hospital Dr. Hasan Sadikin Bandung. The research start from May 1st until November 30th 2012. All sample cases histopathology is seen the image of the Sydney sistem classification, after that are staining with immunohistochemical with antibody monoclonal Hp was performed to confirm the presence of the Hp. Results Immunohistochemical staining result obtained from 42 cases of the bacteria Hp positive and 18 negative cases the bacteria Hp. Chronic inflammation in chronic gastritis with Hp with chronic gastritis without Hp bacteria are in category 3 (limfosit cell more than 20/10HPF) as much as 88,1% and 88,9%. Conclusion Histopathological picture of chronic gastritis with Hp bacteria did not differ with chronic gastritis without Hp bacteria. Key words: Chronic gastritis, Helicobacter pylori, Sydney system.
Pemeriksaan Real Time-Polymerase Chain Reaction (RT-PCR) pada Granuloma Well Organized dan Poorly Organized Limfadenitis Tuberkulosis Dianti Lestari; Birgitta M Dewayani; Abdul Hadi Hassan; Bethy S Hernowo
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Introduction Tuberculous lymphadenitis (TBL) was the most common form of extrapulmonary tuberculosis. The diagnose of TBL was based on the tubercle formation that consist of granuloma and caseous necrosis. There were 2 type of granuloma, well organized (WOG) and poorly organized granuloma (POG). Well organized granuloma were characterized by varying amount of eosinophilic necrosis surrounded by granuloma composed of mature epithelioid macrophages, mainly Langhans type giant cells and a mantle of lymphocytes and fibrous tissues. Poorly organized granuloma were characterized by central area of sparse coarse necrosis with nuclear debris and often polymorphonuclear granulocytes, the granuloma had an ill-defined mantle with mixed cells composed of macrophages, lymphocytes and plasma cells, only few giant cells were seen and there was little or no fibrosis. Inflamatory granuloma also can be found in non TBL, especially POG. The purpose of this study was to find out the etiology of WOG and POG based on the RT-PCR result. Methods This study used 30 formalin-fixed paraffin-embedded tissue blocks from patients who were histopathologically diagnosed as TBL with hematoxillin eosin (HE) staining, consist of 15 samples WOG and 15 samples POG. This study performed RT-PCR to all cases in order to find out the etiology of GWO and GPO and to know if there will be any differences in RT-PCR result between WOG and POG. Results Of the 15 WOG cases, 10 (67%) cases were RT-PCR positive and 5 (33%) cases were RT-PCR negative, whereas 15 cases POG consist of 8 (53%) cases were RT-PCR positive and 7 (47%) cases were RT-PCR negative. Both of cases WOG and POG was analyzed, according to the statistical analysis had found that there were no differences RT-PCR result between WOG and POG significantly (p>0.05). Conclusion There were no differences in RT-PCR result between histopathologic feature of WOG and POG. Key words : Poorly organized granuloma, RT-PCR, tuberculosis lymphadenitis, well organized granuloma.
Prevalensi Infeksi Human Papilloma Virus Tipe 16 dan 18 serta Lesi Prakanker dari Pemeriksaan Pap smear Berbasis Cairan pada Pasien dengan Infeksi Human Immunodeficiency Virus Iin Indrayani Maker Luh Putu; Moestikaningsih .; Ketut Suwiyoga; Mona Mariana
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Background Bali province is the sixth highest Human Immunodeficiency Virus (HIV) prevalence in September 2012, which was 5871 cases, among them were 1227 women. HIV infected women with latent Human Papilloma Virus (HPV) in cervical uterine will have 10-20 times risk to develop cervical precancerous and cancer. The high risk group of HPV including HPV type 16 and 18. The aim of this study is to get the epidemiologic data of HPV type 16 and 18 prevalency and precancerous cervical lesion prevalency from liquid based pap smear of HIV infected women. Methods This was a descriptive prospective cross sectional study. Fifty samples were collected by concecutive sampling from liquid based pap smear of HIV infected women. Two smears were made from each sample, stain with Papanicolaou, then examination of HPV (especially type 16 and 18) was conducted by Polymerase Chain Reaction (PCR) from the remaining sample of each patient. The results of pap smear and PCR examination were grouped into whether there is positive or negative of HPV infection, type of HPV, positive or negative precancerous lesion and age groups. Results From 50 samples, 60% were HPV positive (13.3% type 16, 10% type 18, and 6.7% type 16 and 18). From 30 HPV positive samples, there were 11 (11.3%) precancerous lesions, and from 20 HPV negative samples, there were 1 (5.0%) precancerous lesions. The youngest HPV infected women with precancerous lesion was 19 years old. Conclusion Prevalency of HPV was found in more than half HIV infected samples, which type 16 was found higher than type 18. Precancerous lesions were higher in HIV with HPV infected women. Key words : Cervical precancerous lesion, Human immunodeficiency virus, human papilloma virus, liquid based pap smear, PCR, type 16 and 18
Tinjauan Histopatologik Penyakit Trofoblastik Gestasional di Sumatera Utara pada Tahun 2010-2013 . Soekimin
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Background Gestational trophoblastic disease (GTD) was a heterogeneous group of diseases associated with pregnancy, including partial mole, complete mole, invasive mole, choriocarcinoma and placental site trophoblastic tumor. The incidence of GTD varies in different parts of the world. The malignant potential of this disease was higher in South East Asia in comparison to western countries. The aim of the study was to view the variety of histopathological feature of GTD in Medan. Methods This study was a descriptive study, all cases of the gestational trophoblastic cases from Januari 2010 to June 2013 available at the Department of Anatomical Pathology Faculty of Medicine, University of North Sumatera were included in this study. Results Gestational Trophoblastic Disease mostly found in women in their reproductive period. Most patients were over than 40 years (26 cases, 50%), followed by ages between 20 and 40 years (25 cases, 48.08%) and an age less than 20 years (1 case, 1.92%).Histologic classification of all GTD cases revealed most of the cases were partial hydatidiform mole (35 cases, 67.31%), complete hydatidiform mole (8 cases, 15.38%), invasive mole (3 cases, 5.77%), high risk mole (4 cases, 7.69%); and placental site trophoblastic tumors (2 cases, 3.85%) Conclusion Hydatidiform mole was the most type of gestational trophoblastic disease. In this study the partial mole was more frequent than complete mole. Key words : complete mole, gestational trophoblastic disease, hydatidiform mole, partial mole.
Ekspresi Ki-67 dan HER-2/neu Berhubungan dengan Derajat Histopatologik Karsinoma Payudara Invasif No Special Type (NST) Sanggam B Hutagalung; I Ketut Mulyadi; I Gusti Alit Artha
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Background The role of Ki-67 and HER-2 /neu protein as a prognostic and predictive factor in invasive ductal breast carcinoma of no special type has been studied since the last decade. The aim of this study was to evaluate correlation between expression of Ki-67 and HER-2/neu with histopathological grade of invasive carcinoma of no special type breast. Methods This study was a cross-sectional study, which performed on paraffin embedded tissue of patients with invasive breast carcinoma of no special type who were diagnosed between Juli 2012 to September 2013. Histopathological grade was investigated by hematoxillin eosin and immunohistochemistry stain were made for Ki-67 and HER2/neu. Then the correlation between Ki-67 and HER-2/neu overexpression with histopathological grade tested by Spearman Correlation. Results We found 60 samples, mean age was 55 years old (range 28-71 years). According to histopathological grade of the tumor, we found cases of low grade were 29 cases (48.3%) and high grade were 31 cases (51.7%). There was significant positive correlation between Ki-67 overexpression and high histopathological grade of tumor r=0.650; p=0.000). There was significant correlation between HER-2/neu overexpression and high histopatho-logical grade of tumor (r=0.311; p=0.016). Conclusion It was concluded that the expression of Ki-67 and HER-2/neu have significant correlation with histopathological grade of invasive ductal breast carcinoma of no special type. Key words: HER-2/neu, histopathological grade, invasive ductal breast carcinoma no special type, Ki-67.
Ekspresi P53, Bcl-2 dan Nm23-H1 pada Karsinoma Ovarium Tipe Serosum dan Musinosum serta Hubungannya dengan Stadium dan Derajat Histologi Rusnita .; Prijono Tirtoprodjo; Irianiwati .
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Background Ovarian carcinoma is the second most common malignancy of the female genital tract, following endometrial carcinoma. Highly mortality of ovarian carcinoma is primarily due to difficulties in diagnosing early stage disease. Metastatic process is influenced by many factors, including molecular factors p53, Bcl-2 and nm23- H1. p53 is a tumor suppressor gene that acts to control cell proliferation, while Bcl-2 is an oncoprotein that inhibit apoptosis, which is also associated with response to chemotherapy. Mutation of p53 gene might inhibit Bcl-2 gene therefore stimulate cell proliferation and metastasis. nm23-H1 gene is metastatic inhibitor and decrease the level or expression of these genes will increase the risk of metastasis. p53, Bcl-2 and nm23-H1 expression of ovarian carcinoma need to be investigated in order to obtain more accurate prognosis. This study aimed to investigate p53, Bcl-2 and nm23-H1 expression in Serous and Mucinous ovarian carcinoma and to determine their association with Staging and Grading . Methods Design of this research was a cross sectional design. Subjects used in this study were 50 paraffin-embedded tissues of epithelial ovarian carcinoma, which had been taken from Dr. Sardjito General Hospital (Pathology Laboratory archive). Samples were processed with immunohistochemical staining (IHC) methode using monoclonal antibody anti p53, bcl-2, and nm23-H1. Different expression of p53, Bcl-2, nm23-H1 among samples and their association with staging and grading was analyzed with chi-square. Results p53 expression was associated with grading but not with staging of serous ovarian carcinoma and this protein was not expressed in mucinous ovarian carcinoma. Both Bcl-2 and nm23-H1 expression was not associated with grading and staging of serous ovarian carcinoma. In mucinous ovarian carcinoma, bcl-2 and nm23-H1 expression was not associated with staging. Conclusion Bcl-2 expression was not different among early stages of serous and mucinous ovarian carcinoma. nm23-H1 expression was not significantly different with grading and staging of both ovarian carcinoma. Key words : Bcl-2, mucinosum, nm23-H1, ovarian carcinoma, p53, serosum.

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