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Journal of Stem Cell Research and Tissue Engineering

JSCRTE
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Published by Universitas Airlangga

ISSN : 26141264 EISSN : 26141256 DOI : https://dx.doi.org/10.20473/jscrte

Core Subject : Health, Science, Engineering,

Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

Journal of Stem Cell Research and Tissue Engineering (JSCRTE) is published by Stem Cell Research and Development Center, Airlangga University. Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. Special focus of JSCRTE is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines. Articles that go through the selection process will be review by peer reviewer or editor. The journal is published regularly twice a year in December and May. Every publication consists of 60-70 pages and 5 scientific articles in the form of research, study literature, and the case study in English. The contributors Journal of Stem Cell Research and Tissue Engineering are Stem Cell researchers, lecturers, student and practitioners that came from Indonesia and abroad.

Arjuna Subject : Kedokteran - Imonologi dan Alergi

Articles 5 Documents

Search results for , issue "Vol. 1 No. 1 (2017): Journal of Stem Cell Research and Tissue Engineering" : 5 Documents clear

THE EFFECT OF ALLOGENIC FREEZE DRIED PLATELET-RICH PLASMA IN RESPONSES INFLAMMATION REACTION OF RABBIT rachmawati, trio
Journal of Stem Cell Research and Tissue Engineering Vol. 1 No. 1 (2017): Journal of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

This study aims to analyze the effects of allogenic freeze dried platelet-rich plasma in responses inflammation reaction of rabbit. The designs of this study are one group pretest posttest conducted to determine the effect of freeze drying on levels of TGF-β1 PRP and post test only control group design conducted to determine the effect of allogenic freeze dried PRP. Nine samples of PRP which examined levels of TGF-β1 before and after freeze drying were obtained from blood centrifugation of three rabbits. These nine samples were used as allogenic donor which injected intramuscularly in nine rabbits for the treatment groups. The control group used nine rabbits which was injected intramuscularly using autologous PRP. Both groups were observed inflammatory response. Measurement of TGF-β1 levels before and after freeze drying were tested statistically using T- test dependent. Data inflammatory response were tested statistically using T- test independent. The results showed that no effect of freeze drying process on levels of TGF-β1. Allogenic freeze dried PRP did not cause an iflammatory response.Keywords : autologous, allogenic, freeze dried platelet rich plasma, transforming growth factor- β1.

TISSUE ENGINEERING IN MAXILLOFACIAL BONE RECONSTRUCTION Kamadjaja, David
Journal of Stem Cell Research and Tissue Engineering Vol. 1 No. 1 (2017): Journal of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Maxillofacial bone defects due to tumor resection, trauma or infections should be reconstructed to maintain the bone continuity in order to preserve its masticatory, speech and esthetic functions. Autogenous bone graft have been the gold standard for mandibular defects reconstruction, however, it is associated with limitation in volume and availability as well as the donor site morbidities. Tissue engineering approach has been proved to be a good alternative to overcome the limitation of autogenous bone graft. Tissue engineering studies have been conducted combining various sources of mesenchymal stem cell, scaffolds, and or signaling molecules. The paper aims to provide information on the development of bone tissue engineering researches to reconstruct bone defects through results of numerous studies obtained in the English literature. As the conclusion, bone tissue engineering is a potential approach to reconstruct maxillofacial bone defects. Keywords: scaffold,osteoconduction, mesenchymal stem cell, bone regeneration, bone integration

FABRICATION OF PCL-COLLAGEN NANOFIBER USING CHLOROFORM-FORMIC ACID SOLUTION AND ITS APPLICATION AS WOUND DRESSING CANDIDATE armeda, tri prasetyo
Journal of Stem Cell Research and Tissue Engineering Vol. 1 No. 1 (2017): Journal of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

In this study, polycaprolactone-collagen nanofiber was prepared with 10% w/v composition using a mixture of chloroform-formic acid. PCL was dissolved in chloroform while collagen was dissolved in formic acid. This research carried out optimization of electrospinning parameters such as flow rate, running time, and collector type to obtain optimum and suitable nanofiber to be applied as wound dressing. The most optimum nanofiber is made with flow rate 0.01 μL/h, running time is 3 hours, and using cylinder collector type. Characterization was performed for five different types of PCL-collagen nanofiber with different treatment, which nanofiber made with cylinder collector, plate collector, addition ofcitric acid, heating treatment, and nanofiber without the addition of collagen. PCL-collagen nanofiber produces smaller diameter about 200 - 600 nm. Based on the test of mechanical properties, addition of collagen causes its mechanical properties to be lower when compared to addition of crosslinking agents by heating or citric acid. The cytotoxicity test was carried out for PCL, PCL-collagen withaddition of citric acid, and PCL-collagen nanofiber treated by heating. PCL was chosen to compare the effect of collagen addition onnanofiber against cell viability. Collagen has an important role for growth, proliferation, and differentiation of cells in tissue engineering. PCL-collagen nanofiber which treated by heating provides better viability of 83.09% while compared to nanofiber with addition of citric acid, because citric acid acidic properties causing the environment around nanofiber have an extreme pH, it may affect the growth of cells and reduce its viability.Keywords:Nanofiber, PCL, collagen, electrospinning, wound dressing, MTT Assay

PROTOTYPE DESIGN OF LYMPHOCYTE TCD4+ RESISTANT AGAINST HIV INFECTION GENERATED FROM PERIPHERAL BLOOD HAEMATOPOIETIC STEM CELL (PBMCs) By DELETION OF 32 bp CCR5 ENCODING GENE Purwati, Purwati
Journal of Stem Cell Research and Tissue Engineering Vol. 1 No. 1 (2017): Journal of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

AIDS epidemic has spread to all parts of Indonesia and currently more than 150 countries reported the existence of HIV/AIDS from around the world. Additionally, HIV/AIDS treatment using ARV drugs also find obstacles that must be faced in terms of host, environment and the agent. The objective of this study was to generate lymphocytes TCD4+ that are resistant to HIV infection generate from PBMCs through by deletion of 32 bp CCR5 encoding gene. In principle, this study was done in three steps. First, isolation, culture and purification of lymphocyte TCD4+ from PBMC (Mather, 2008; Rantam, et.al., 2009). Second, lymphocyte TCD4+ characterization by PCR with primer F 5’CAAGTCGAGCGCCCCGCAAGGGG-3, R 5’GTCCGAGTGTGGCTGATCATCC-3 (Thomsen, et.al., 2002; Yuwono, 2006; Hall and Ziedonis 2007; Purwati, et.al., 2009). Third, designing of lymphocyte TCD4+ prototype which was resistant to HIV infection by deletion of 32 bp CCR5 full gene. Results: Twenty-four hours after culture, there were abundant cell growths. TCD4+ lymphocytes from isolated and cultured 10 ml PBMC were found to be 2 x 107. Phenotype characterization of TCD4+ lymphocyte provided positive results, while the genotype showed similarities to that in corresponding gene bank of CCR5 variant A and variant B. Prototype of HIV resistant TCD4+ lymphocytes was made by nucleotide deletions in conserved areas, at position 554-576 bp, using restriction enzymes EcoRI checked using PCR and sequencing. In conclusion, prototype design of HIV resitent TCD4+ lymphocytes is obtained through the deletion of 32 bp CCR5 encoding full gene at GTCAGTATCAATTCTGGAA GAATTT CCAGACA using EcoRI enzyme.Keywords: HIV/AIDS, resistant TCD4+ lymphocytes, mutant 32 bp CCR5, PBMCs, deletion

THE EFFECT OF INTRA-ARTICULAR APPLICATION OF ALLOGENIC MESENCHYMAL STEM CELL COMBINE WITH VEGF TO GRAFT TUNNEL HEALING AND AUTOGRAFT TENDON INTEGRATION IN ACL RECONSTRUCTION; A BIOMECHANICAL STUDY Ferdiansis, Ferdiansis
Journal of Stem Cell Research and Tissue Engineering Vol. 1 No. 1 (2017): Journal of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Graft-tunnel healing is the most determination factors in successful of Anterior Cruciate Ligament (ACL) reconstruction. The application of bone marrow derived mesenchymal stem cell (MSC) and vascular endothelial growth factor (VEGF) are one of integration biological augmentation method that often used in ACL reconstruction. Combination intra-articular post ACL reconstruction is expected to accelerate healing time and integration strength of tendon graft that used in bone tunnel. This method is experimental laboratory using animal model. The research is randomized post test only controlled group design. Five New Zealand white rabbit knee are used for ACL reconstruction with harmstring tendon graft and treated with combination allograft MSC and VEGF intra-articular, while five other rabbit knee as control without treatment. The evaluation is tensile test in third and six weeks post operation. Data was analyzed statistically and comparatively to compare the influence of MSC and VEGF to integration strength of graft tunnel healing. All the samples from treatment and control group found no complication after surgery. On third weeks evaluation, found a difference in failure tension load in both groups but not statistically significant (p>0,05), while on six weeks evaluation, found a statistically significant difference. Treatment group has a failure tension load higher than control group. While failure type of ACL tendon graft on 3 weeks evaluation, only 2 of 5 graft have pullout failure in treatment group. However, at three weeks in control group, the failure type of the tendon graft was a midsubtance rupture in intra-articular part during biomechanical tension test. The use of BM-MSC and VEGF intra-articular can increase tension failure load. It is expected that combination of BM-MSC and VEGF can increase integration process between bone graft and healing post ACL reconstruction, so that rehabilitation and mobilisation can be done earlier.Keywords: Graft-tunnel healing, ACL reconstruction, Vascular Endothelial Growth Factor (VEGF), Bone Marrow Derived Mesenchymal Stem Cell (BMMSC)

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Contact Name
Fika Kharisyanti

Contact Email
fikakharisyanti@gmail.com

Phone
+6282232687366

Journal Mail Official
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Editorial Address
Ruang Stem Cell, Gedung Lembaga Penyakit Tropis Lantai 2, Kampus C Universitas Airlangga

Location
Kota surabaya,

Jawa timur

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Fika Kharisyanti

Contact Email fikakharisyanti@gmail.com

Phone +6282232687366

Journal Mail Official -

Editorial Address Ruang Stem Cell, Gedung Lembaga Penyakit Tropis Lantai 2, Kampus C Universitas Airlangga

Location Kota surabaya, Jawa timur INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Fika Kharisyanti

Contact Email
fikakharisyanti@gmail.com

Phone
+6282232687366

Journal Mail Official
-

Editorial Address
Ruang Stem Cell, Gedung Lembaga Penyakit Tropis Lantai 2, Kampus C Universitas Airlangga

Location
Kota surabaya,

Jawa timur

INDONESIA