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Contact Name
Fika Kharisyanti
Contact Email
fikakharisyanti@gmail.com
Phone
+6282232687366
Journal Mail Official
-
Editorial Address
Ruang Stem Cell, Gedung Lembaga Penyakit Tropis Lantai 2, Kampus C Universitas Airlangga
Location
Kota surabaya,
Jawa timur
INDONESIA
Journal of Stem Cell Research and Tissue Engineering
Published by Universitas Airlangga
ISSN : 26141264     EISSN : 26141256     DOI : https://dx.doi.org/10.20473/jscrte
Journal of Stem Cell Research and Tissue Engineering (JSCRTE) is published by Stem Cell Research and Development Center, Airlangga University. Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. Special focus of JSCRTE is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines. Articles that go through the selection process will be review by peer reviewer or editor. The journal is published regularly twice a year in December and May. Every publication consists of 60-70 pages and 5 scientific articles in the form of research, study literature, and the case study in English. The contributors Journal of Stem Cell Research and Tissue Engineering are Stem Cell researchers, lecturers, student and practitioners that came from Indonesia and abroad.
Articles 5 Documents
Search results for , issue "Vol. 2 No. 2 (2018): Journal of Stem Cell Research and Tissue Engineering" : 5 Documents clear
The Effect Of Immersion Time Variation in Polyvynyl Piprolidone Against Characteristics Of Scaffold Biocomposit Of Bacterial-Hydrocysiatatic Cellulose as Candidate indrio, ludita
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 2 (2018): Journal of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.188 KB) | DOI: 10.20473/jscrte.v2i2.11893

Abstract

Bone defects due to trauma, tumors, congenital abnormalities, degeneration and other diseases are still major problems in the field of orthopedics and traumatology. Based on data in Asia, Indonesia is the country with the highest number of fracture sufferers, there are as many as 300-400 cases of bone surgery per month in hospitals. Dr. Soetomo Surabaya (Gunawarman et al, 2010). Repair of damaged bones can be overcome with material that can accelerate the process of bone healing (bone healing). This research was conducted to synthesize hydroxyaparite bacterial cellulose scaffold as a candidate for bone healing. Bacterial cellulose as a matrix was synthesized by culturing Acetobacter xylnum, while hydroxyapatite as filler was synthesized by immersion into a solution of CaCl2 and Na2HPO4, the scaffold formation process using freeze dried method. Composite formation was varied by immersion in Polyvynil pirrolidone (PVP) for 0, 1, 2, 3, 4 days. Furthermore, samples were characterized using FTIR-Spectroscopy showing the presence of carbonates containing apatite crystals in all five samples.
Potential Hollowfiber Polyurethane-Collagen of Chitosan Coatings As a Nerve Graft for the Therapy of Peripheral Nerve Injuries in Limb Paralysis Maulida, Hendita
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 2 (2018): Journal of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.627 KB) | DOI: 10.20473/jscrte.v2i2.11892

Abstract

Peripheral nerve injury with a gap of 5–30 mm can cause permanent paralysis because it causes an axon to break up. The distance between axons of more than 1-2 cm requires a graft in the form of a nerve connector to fix it. Synthesis of chitosan coated polyurethane-collagen hollowfiber has been carried out as an accelerator for healing peripheral nerve injury. The results of Fourier Transform Infra Red (FTIR) analysis showed a cross link between chitosan and glutaraldehyde seen in the shift of wave numbers from 1080-1100 cm-1 to 1002 cm-1. The degradation test results showed that the sample experienced a decrease in mass after being immersed in Simulated Body Fluid (SBF) for 7 days. Polyurethane can be degraded in the body after 30 days. This is in accordance with the mechanism of the nerve which regenerates 1 mm per day or 1 inch per month. Scanning Electron Microscope (SEM) analysis showed that the diameter of the hollowfiber was 2.021-2.032 mm which corresponds to the peripheral nerve diameter of 1.5-3 mm and the pore size of the wall is 31.33-39.65 μm. The results of this study are expected to provide the theoretical basis for the use of chitosan polyurethane-collagen coating composites as nerve grafts for the treatment of peripheral nerve injuries that have biocompatible properties, can regenerate and are easily degraded and provide alternative solutions for nerve graft needs that are more economical and easier to manufacture so widely produced in Indonesia.
Cellullar Plasticity and Dedifferentiation: A Link Between Cancer Stem Cells, Hypoxia, Cell Injury, and Inflammation Wijaya, Andi Yasmin
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 2 (2018): Journal of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.9 KB) | DOI: 10.20473/jscrte.v2i2.11655

Abstract

Cellular plasticity is the concept of bidirectional dynamics change cells differentiation degree which involved in the regeneration, repair and tissue turnover along the organism livespan. Cellular plasticity and dedifferentiation process are well documented in the discovery of iPCSs by introducing several transcriptional factors known as Yamanaka factor to terminally differentiated somatic cells and reverted into pluripotent state as the ESCs. iPSCs are able to exhibit ESCs differentiation potential which could produce ectodermic, mesodermic, and endodermic cell lineage. In tumour biology, the tumour plasticity also have a similar regulation and play an imporant role for maintaining tumour integrity and survival, particularly in maintaining CSCs population. Various study of cellular plasticity regulation has shown that various factors are involved, in example hypoxia, cell injury, and inflammation. Cells respond to hypoxia, cell injury, and inflammation by chemoattractant which attract repair cells to homing towards injured sites. The homing mechanism of stem cells involved EMT to facilitates migration of stem cells towards injured sites, thus leading to tissue regeneration. On the other hand, cancer metastasis also showed a connection with EMT process. EMT which showed a change in cell properties are linked to dedifferentiation and hypoxia response. Hypoxia condition has been known to preserve and both normal stem cells and CSCs stemness. HIF which protected from degradation in hypoxia condition interact with DNA by binding to HRE. HRE activation trigger transcription of numerous signalling protein which involved in stemness, cell proliferation and survival. Therefore it is concluded that cell injury, hypoxia, and inflammation could programmed cells to undergo dedifferentiation process and involved in EMT regulations. CSCs which resides insides heterogeneous tumour cells population are though to be dynamicly regulate itself in the quietscent and active state through dedifferentiation like the normal stem cells. Understanding how CSCs regulates its active an quietscent state dynamics could provide an important information for novel CSCs targeted therapy development. 
Brain Derived Neurotrophic Factor Levels in Aged Rats Post-Systemic Human Mesenchymal Stem Cell Administration dwijayanti, adisti
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 2 (2018): Journal of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.863 KB) | DOI: 10.20473/jscrte.v2i2.11895

Abstract

Brain-Derived Neurotrophic Factor (BDNF) levels were affected by aging. Brain BDNF levels were known to decrease along with advanced age thus correlated with any diseases such as cognitive impairment and Alzheimer. Mesenchymal Stem Cell (MSC) is one of the potential modalities actively investigated against age-related diseases. This study evaluated the effect of human MSC administration to brain BDNF levels in aged rats. Intravenous injection of 10 million per body weight human MSC were given four times in 3 months interval to 22-24 months old female and male Spraque–Dawley rats. As control group, aged rats were injected by normal saline at the same volume and frequencies. Moreover, young 3-6 months rats also examined as negative control.  By the end of the experiment, we analyzed three rats from each group. Brain BDNF levels were measured by enzyme-linked immunosorbent assay and normalize to the protein levels. One-way ANOVA and LSD post hoc analysis was performed to compare the differences between groups. BDNF levels in male appeared similar between young, aged, and MSC treated groups. Meanwhile, control aged female groups had significantly lower BDNF levels compared to young (p = 0.019) and MSC-treated aged rats (p = 0.001). There was no difference of BDNF levels between young and MSC-treated aged in female rats (p = 0,068). Both sex had similar BDNF levels (p = 0.249) in control-aged groups. In contrast, female young and MSC-treated aged rats achieved significantly higher BDNF levels (p = 0.009 and p <0.001) compared to the male groups, respectively. These results suggest that human mesenchymal stem cell intravenous injection can increase brain BDNF levels in female aged rats.
Stem Cell from Human Exfoliated Deciduous Teeth (SHED) versus Human Umbilical Cord Blood Mononuclear Cells (cbMNC) Transplantation in Neural Damage Reduction in Rat Model of Cerebral Ischemia ramli, yetty
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 2 (2018): Journal of Stem Cell Research and Tissue Engineering
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (239.648 KB) | DOI: 10.20473/jscrte.v2i2.11896

Abstract

Ischemic stroke is one of major cause of mortality and disability in Indonesia. Stem Cells are considered as a promising therapy for ischemic stroke. In this study, we compared therapeutic potency of Stem cell from human exfoliated deciduous teeth (SHED) and Human umbilical cord blood mononuclear cell (cbMNC) using rat models of ischemic stroke. Following middle cerebral artery occlusion (MCAO), twenty male wistar rats were divided into four groups : normal rats (n=5), rats undergone permanent MCAO (n=5) as the control (stroke) group, rats undergone permanent MCAO and SHED transplantation (n=5) and rats undergone permanent MCAO and cbMNC transplantation (n=5) as the treatment group. SHED transplantation was performed at the acute phase after MCAO by intravenous injection. Histopathological evaluation of the neuron death ratio with hematoxylin and eosin staining confirmed that there was no significant differences at comparative study of neuron death ratio in rats transplanted with SHED and rats transplanted with cbMNC (p=0,81). SHED and cbMNC transplantation at acute stroke showed reduction in the neuron death ratio in the brain of rat models with ischemic stroke, and may provide an opportunity for neuroprotection and neural regeneration after ischemic stroke.

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