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Nusantara Science and Technology Proceedings
Published by Future Science
ISSN : -     EISSN : 26229692     DOI : -
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Articles 13 Documents
Search results for , issue "Join Proceeding "Basic and Applied Science Conference (BASC) 2021 " : 13 Documents clear
Effect of Surfactant Type Modifications, Glucose Inducer Concentrations, and Mineral Sources towards Lipase Enzyme Activity of Aspergillus niger ITBCC L74 on Rice Bran Substrate Ajeng Ardani; Shinta Rismayanti; Hamid Abdillah
Nusantara Science and Technology Proceedings Join Proceeding "Basic and Applied Science Conference (BASC) 2021 & 1st Education Research and Appli
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1101

Abstract

A surface-active agent is a substance added to a liquid to increase its spreading properties by lowering the surface tension. On the other hand, an inducer is a substance to triggers the production of lipase enzymes from bacteria. This study aims to formulate the microbial growth medium to produce high activity of lipase through modification of surfactant type, glucose inducer concentrations, and mineral sources using Aspergillus niger as a microbial starter and rice bran as substrate. A solid fermentation was built and prepared in this study for lipase production. The titrimetric method using NaOH solution was used to determine mol of free fatty acids per minute, type of surfactant, the optimal glucose concentration, and mineral sources on lipase enzyme activity. Tween 80 and Tween 20 are lipid-based surfactants used in this experiment. The best type of surfactant was Tween 80 with 1% of glucose inducer. It produced a lipase activity of 2.53 U/mL. In contrast, Tween 20 with 0.75% glucose inducer produced a lipase activity of 2.27 U/mL. FeSO4 and CaCl2 were the inorganic mineral sources used in this study. The best result was found at 0.6% of FeSO4, it can generate enzyme activity of 2.13 U/mL.
Analysis of potential genes for the development of potatoes (Solanum tuberosum) with heat stress-tolerance Bernadetta Rina Hastilestari
Nusantara Science and Technology Proceedings Join Proceeding "Basic and Applied Science Conference (BASC) 2021 & 1st Education Research and Appli
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1102

Abstract

Potato (Solanum tuberosum) is a widely cultivated plant in the highlands. Low temperature, such as temperature at night, is required for tuber formation. However, global warming has increased global temperature and causing a decrease in crop yields. To meet the growing demand for potatoes, the planting area needs to be expanded. Planting can be done at the medium-altitude or even lowland by developing potato varieties that are well-adapted to a higher temperature. The first step to achieve this goal is to analyze the potential genes for potato breeding by comparing the data from the microarray. Genes related to heat shock proteins affect the resistance of potato plants towards the increasing temperatures in the environment.
Cytotoxicity Effects of Vitamin C on Cancer Cells MCF-7 Endang Purwaningsih; Samsul Mustofa; Pendrianto
Nusantara Science and Technology Proceedings Join Proceeding "Basic and Applied Science Conference (BASC) 2021 & 1st Education Research and Appli
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1103

Abstract

Vitamin C (ascorbic acid) is a powerful antioxidant that can reduce the effects of oxidative stress. One of the diseases caused by oxidative stress is cancer. The study aimed to assess the cytotoxic effect of vitamin C on MCF7 cancer cell cultures. This study used an in vitro laboratory experiment using MCF-7 breast cancer cell culture. MTS assay method was used to test the dose variance of vitamin C (l-ascorbic acid) against MCF-7 cultures. The MTS test used the CellTiter 96 Aqueous One Solution Cell Proliferation Assay kit. After that, 24 and 48 hours of culture were observed using test doses, 0 (negative control, K(–)), 10, 20, 40, 80, 160, 320 g/mL. The results showed the higher the dose given, the lower the viability of MCF7 cells, both at 24 and 48 hours of incubation. The lowest viability was obtained at 86.75% at a dose of 320 g/mL during incubation for 24 hours and 7.95% at a dose of 320 g/mL during incubation for 48 hours. In conclusion, vitamin C has a cytotoxic effect on MCF-7 cell culture with an IC50 value of 140 g/mL. Further research is needed to determine the effectiveness of vitamin C on the expression of the telomerase enzyme in breast cancer cells, especially MCF-7.
Association of XRCC1 genetic polymorphism with the risk of thyroid cancer in Indonesia Harry Nugroho Eko Surniyantoro; Nabilah Adzra Fahlevi; Esa Savitri; Tur Rahardjo; Rita Maliza; Ayu Rosemeilia Dewi; Yustia Tuti; Nastiti Rahajeng; Yanti Lusiyanti; Nur Rahmah Hidayati
Nusantara Science and Technology Proceedings Join Proceeding "Basic and Applied Science Conference (BASC) 2021 & 1st Education Research and Appli
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1104

Abstract

One of the important DNA repair pathways is base excision repair (BER), which plays in the human body to maintain DNA integrity, prevent cancer and DNA damage. X-ray repair cross-complementing group 1 (XRCC1) is the most important DNA repair protein in base excision repair (BER) pathways and has been reported to have a relationship with the risk of developing various cancers. The study was purposed to assess the genetic polymorphism of XRCC1 exon 6 and 10 as a risk factor for thyroid cancer. A total of 90 participants were enrolled in this study, consisted of 30 thyroid cancer patients as a case group and 60 non-cancer patients as a control group. Examination of XRCC1 genotypes was carried out by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method and statistical analysis using a Chi-square test. In this study, we reported the frequency of XRCC1 genetic polymorphism was not significantly different between cancer patients and control groups both in exon 6 and 10, and we predict that these polymorphisms were not a risk factor for thyroid cancer (P-value>0.05). In conclusion, both mutant variants of XRCC1 exon 6 and 10 are not risk factors for thyroid cancer. In further studies, it is necessary to assess genetic polymorphisms in populations with controlled non-genetic factors, such as diet, lifestyle, and environmental factors.
The Use of Instagram as Media for Stunting Campaign: Analysis on Gen Z Knowledge Lathifah Dzakiyyah Zulfa
Nusantara Science and Technology Proceedings Join Proceeding "Basic and Applied Science Conference (BASC) 2021 & 1st Education Research and Appli
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1105

Abstract

The long-term impacts of stunting are very diverse, such as cancer, diabetes, anemia, or stupidity, and will interfere with the productivity of the individual itself. The right educational media is needed to raise awareness of the young generation so that changes in the mindset of choosing food (nutritional intake) can start now and prevent stunting in future offspring. Z generation students as samples were given education through Instagram uploads from the @ChangeTheCircle account with the tag #ChangeTheCircle, after previously being asked to do a pretest. The pretest-posttest scores' mean from the intervention and control groups were then tested for significance by using paired t-test. Not forgetting to see a difference in initial ability, the researcher tested the unpaired t-test and U test on the two groups' pretest and posttest. The intervention group (P <0.05) showed a significant difference between pretest and posttest scores while the control group did not. The difference in initial ability as measured through the comparison of pretest scores was counted insignificant, so there was no bias from the sample's initial knowledge in the control or intervention groups to the posttest results. The campaign on stunting issues through Instagram posts with the tag #ChangeTheCircle has an impact on the reader's ability to answer questions, indicating that this campaign has succeeded in increasing their knowledge of stunting.
Development of Selective Media for Komagataeibacter intermedius and Dekkera bruxellensis from a Mixed Culture Ferren Pratama; Putu Virgina Partha Devanthi; Katherine Kho
Nusantara Science and Technology Proceedings Join Proceeding "Basic and Applied Science Conference (BASC) 2021 & 1st Education Research and Appli
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1106

Abstract

Komagataeibacter intermedius and Dekkera bruxellensis are some of the most commonly found bacteria and yeast, respectively, during Kombucha fermentation. The development of selective media for separating both species is essential to make an accurate cell enumeration. This study aimed to develop a selective media for the enumeration of K. intermedius and D. bruxellensis from a mixed culture sample. Hestrin and Schramm (HS) agar and Potato Dextrose Agar (PDA) were chosen as the enumeration media for K. intermedius and D. bruxellensis, respectively. To select for K. intermedius, HS agar was added with various concentrations of cycloheximide (0, 1, and 10 mg/mL), NaCl (2% and 5%), and acetic acid (1%). To select for D. bruxellensis, PDA was added with various concentrations of chloramphenicol (0, 0.3, and 3 ?g/mL) and NaCl (2% and 5%). Each culture (~106 log CFU/mL) was serially diluted and plated on the respective agar media, followed by incubation at 30°C for 1-6 days. The results showed that PDA containing 2% NaCl could completely suppress K. intermedius growth while permitting complete recovery of D. bruxellensis. However, it took 6 days until visible growth was observed by D. bruxellensis. Moreover, HS with the addition of 1% acetic acid successfully inhibited D.bruxellensis while allowing the complete recovery of K. intermedius. The findings suggested that the two media were suitable for separating K. intermedius and D. bruxellensis on agar media, thus allowing a more accurate cell counting in studies involving the mixed cultures of both species.
Towards Clean River Through Distributed Community Based Waste to Energy Aria Aditya; Supriadi Legino; Doddy Hertanto; Hany Stenvil
Nusantara Science and Technology Proceedings Join Proceeding "Basic and Applied Science Conference (BASC) 2021 & 1st Education Research and Appli
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1107

Abstract

Municipal waste is the main source of challenges to ensure a clean river, with the main cause being a lot of its citizens still throw their plastic bags full of waste into the river, as if the river is a public moving garbage container. The purpose of this study is to conduct a benefit-cost analysis of applying the new way of community-based waste treatment (CBWT) that has been successfully applied as a pilot project at various places including the office area of Gerakan Ciliwung Bersih (GCB). The research is a case study analysis method based on real data taken from the ongoing CBWT units that can convert a ton of waste into approximately 300 kg of pellet with around 3,000 kCal/kg bio-coal using microbiological treatment. The case studies in more than 15 places revealed that the capital expenditure of CBWT is around IDR 497,200, 000 and its operational expenditure is IDR 280,970,000 may potentially be profitable to attract small businesses that run these CBWT services. The proposition of this study is the more CBWT business units, the cleaner the river will be and in addition, it will also give social and environmental benefit as it will create employment opportunities and reducing Green House Gasses In this study, the financial, social, and environmental aspects will be calculated by using simulation.
Webinar Learning Strategies: Efforts to Increase Student’s Activeness and Learning Achievements in the New Normal Period in Public Policy Course Evi Susilawati; Imamul Khaira
Nusantara Science and Technology Proceedings Join Proceeding "Basic and Applied Science Conference (BASC) 2021 & 1st Education Research and Appli
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1108

Abstract

Because to improve student activities and learning outcomes in this pan-demic mass is the teacher's main task. To develop these competencies, one option is to use a seminar website or webinar as a learning strategy that can be applied to students. However, little is known about the process of improving student activity and learning outcomes through webinars. The purpose of this study was to increase student activity and learning outcomes during the pandemic in public policy courses through webinar learning strategies. This study uses classroom action research methods which were carried out at the Islamic University of North Sumatra with the participation of 10 students. The instruments used in this study were observation sheets, student assessments, and interview sheets. The results showed that there was an increase in student learning activities, especially in refuting activities or accepting other friends' ideas. The results of this classroom action research show that there is an increase in the activeness and learning achievements of students during the New Normal period in the public policy course using the webinar strategy.
Smart Educenter with Let’s Read Application for Children in Wonopuro Village Maruti, Endang Sri; Isticomah, Binti; Yustiwa, Galuh Mei; Khoiru, Ulifa; Huda, Nur
Nusantara Science and Technology Proceedings Join Proceeding "Basic and Applied Science Conference (BASC) 2021 & 1st Education Research and Appli
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1109

Abstract

Wonopura village is a village located in the hills of Rajakwesi, Dukuh Sidowayah RT.11 RW.03 Sidoharjo Village, Jambon Ponorogo District which is quite remote. The lack of the role of the family and the environment, both schools and the surrounding community is a source of problems for children, especially related to literacy problems. This program aims to help children to improve their digital literacy skills and also make it easier for parents to guide and monitor their children's activities. For this reason, this program is carried out as an effort for Digital Education and Literacy for isolated village children so that they can continue to live life like children in other villages. This program is carried out for 1 month with four meetings on Sundays. Every meeting, gather the children in Wonopuro Village RT.11 RW.03 Dukuh Sidowayah, Sidoharjo Village, Jambon District, Ponorogo Regency. The result of the implementation of this program is that it can improve digital literacy for the children of Kampung Wonopuro. The increase can be seen from the social side, namely increasing children's enthusiasm for learning to develop their potential and achievements during the current pandemic conditions; from an economic point of view, it can increase the attractiveness of the outside community after knowing that Wonopuro Village is to be a regional tourist destination with beautiful natural scenery through ecoliteracy and ecotourism; and from a psychological point of view, it can help increase the learning motivation of the children of Kampung Wonopuro.
The Factors Affecting Soybean Production in Indonesia Using Robust Regression with Least Median of Squares (LMS) Estimation Ratri, Aninda Puspa; Susanti, Yuliana; Slamet, Isnandar
Nusantara Science and Technology Proceedings Join Proceeding "Basic and Applied Science Conference (BASC) 2021 & 1st Education Research and Appli
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1110

Abstract

Soybean is a product with a source of protein that improves the nutrition of Indonesians. The demand for soybeans is increasing, but the domestic production is not sufficient, so that the soybean production in Indonesia must be increased. This study aims to determine the influential factors on soybean production in Indonesia. The data of soybean production in Indonesia had outliers. Outliers cause the residual is not normally distributed so that the assumption of normality is violated. This problem was solved using robust regression. The estimation used was the Least Median of Squares because this estimation has a quite large breakdown point value. The results of the study show that the soybean production in Indonesia was influenced by the field area, the number of soybean seeds, and rainfall. The most influential factor on soybean production is the number of soybean seeds, field area, and rainfall. The attempts that must be conducted by the government to increase soybean production are by having socialization about soybean cultivation and ensuring the availability of soybean seeds in Indonesia.

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