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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

Arjuna Subject : -

Articles 361 Documents

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Identifikasi Cendawan Endofit Menggunakan Teknik Polymerase Chain Reaction (Detection of Endophytic Fungi Using Polymerase Chain Reaction Technique) Tuti Susanti Legiastuti; Tri Aminingsih
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellow leaf curl disease, caused by a member of Begomovirus (Geminiviridae), is one of important diseases of chilli pepper in Indonesia. Exploration of endophytic fungi was initiated in order to find biological control agents for an alternative control strategies of this disease. Isolates of endophytic fungi were collected from chilli pepper growing area in Sleman, Yogyakarta and further identification using molecular technique involving polymerase chain reaction (PCR) and DNA sequencing was performed. DNA fragments of ±500 bp were successfully amplified from 10 fungal isolates by PCR using primer pair ITS1/ITS4, but only 8 DNA sequences was obtained for further genetic analysis. Based on BLASTN analysis the endophytic fungi were identified as having the highest similarity with Pleosporaceae sp. (98%) for H1 isolate, Cercospora nicotianae (100%) for H5 isolate, ercospora piaropi (98%) for H11 isolate, Guignardia mangiferae (99%) for H16 isolate, Geomyces pannorum 95%) for H17 isolate, Diaporthe phaseoloru (99%) for H18 isolate, Dothideomycete sp. (100%) for K3 isolate, and Alternaria longissima (99%) for K10 isolate. Key words: Begomovirus, chillipepper, DNA sequencing, polymerase chain reaction

Penggunaan Bakteri Kitinolitik sebagai Agens Biokontrol Penyakit Busuk Batang oleh Rhizoctonia solani pada Tanaman Kedelai (Utilization of Chitinolitic Bacteria as Biocontrol Agent of Stem Rot Disease by Rhizoctonia solani on Soybean) Andi Khaeruni; Abdul Rahman
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Biological control of stem rot disease caused by Rhizoctonia solani on soybean using chitinolitic bacteria is one of the environmentally friendly control methods of pathogen. The study was conducted to select chitinolitic bacteria and to test their ability to suppress stem rot disease and promote plant growth of soybean. A total of ten chitinolitic bacteria were tested in vitro, and five of isolates i.e: ST21e, SS12b, ST17c, ST27d, and ST26c found to have more than 30% suppression toward R. solani. In planta test under green house condition at six weeks after planting showed that soybean plants inoculated with ST27d and ST17c isolates had significantly lower disease incidence and had increased plant height and leaf number. Both of chitinolitic bacteria isolates are promising as biocontrol agents of stem rot disease caused by R. solani of soybean.Key words: biological control, chitinolitic bacteria, Rhizoctonia solani

Ketahanan Lapangan Padi Gogo terhadap Infeksi Curvularia oryzae Muhammad Taufik; Asniah .; Syair .
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Southeast Sulawesi is known to have a wide varieties of local cultivars of upland rice. Unfortunately, resistance of those local cultivars of upland rice to Curvularia oryzae, one of the most important pathogen in upland rice cultivation, has not been reported. Resistance evaluation conducted in the field showed that local cultivars (Pae Kori, Pae Endokadia, and Pae Enggalaru) have high level of resistance, developing lines B10580E and B11580E have moderate resistance, whereas B11577E and TB368B lines were susceptible to C. oryzae infection.Key words: Curvularia oryzae, disease resistance, upland rice

Penyebab Penyakit Layu pada Tanaman Semangka di Karawang, Jawa Barat Kartini Budiastuti; Efi Toding Tondok; Suryo Wiyono
Jurnal Fitopatologi Indonesia Vol 8 No 4 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Wilt disease of watermelon is an important disease in Indonesia, but determination of the causal agent has not been reported yet. Postulat Koch based approach was conducted to determine the pathogen causing wilt of watermelon from Karawang, West Java. Fungi associated with wilt symptoms were isolated from stem and root, then the fungal colonies were identified based on their morphological characters. Pathogenicity tests were performed using soil media and wet paper. Host range study involved other Cucurbitaceae plants, i.e. cucumber, melon and paria. Three species of Fusarium were successfully isolated from plants showing wilt symptom, i.e. F. oxysporum, F. solani, and F. semitectum. The fungus F. oxysporum consistently caused the most severe wilt symptom on watermelon, but developed no symptom on other tested cucurbits. The two fungi, F. solani and F. semitectum, caused necrotic on roots and stem of watermelon followed by wilting of the plant. The symptom was different than those of the initial symptoms from the field. Therefore it concluded that wilt symptom of watermelon is caused by Fusarium oxysporum f.sp. niveum. Key words: Cucurbitaceae, Fusarium oxysporum f.sp. niveum, species identification, wilt

Pengaruh Empat Galur Bakteri Pemacu Pertumbuhan Tanaman dan Waktu Inokulasi Virus terhadap Keparahan Penyakit Daun Keriting Kuning Cabai Hari Priwiratama; Sri Hendrastuti Hidayat; Widodo .
Jurnal Fitopatologi Indonesia Vol. 8 No. 1 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Plant growth-promoting rhizobacteria (PGPR) has effectively reduced infection of pathogens. Bacillus polimixa BG25, B. subtilis SB3, Pseudomonas fluorescens PG01, P. fluorescens S32, and their combinations were used as seed treatment. Bemisia tabaci (Hemiptera: Aleyrodidae) was used to inoculate Geminivirus at 3, 5, and 6 weeks after planting. Seeds without any bacteria treatment were used as controlled plant. Treatment of PGPR and time of inoculation were significantly effect disease intensity, but not incubation period. Late infection of Geminivirus reduced disease intensity. Based on measurement of plant height, number of branch, and flowers it was concluded that some bacterial treatments was significantly effect plant growth. Key words: Bemisia tabaci, Geminivirus, PGPR, plant growth promoting rhizobacteria

Isolasi dan Identifikasi Secara Molekuler Ganoderma spp. yang Berasosiasi dengan Penyakit Busuk Pangkal Batang di Kelapa Sawit Maria Indah Purnamasari; Cahya Prihatna; Agustin Wydia Gunawan; Antonius Suwanto
Jurnal Fitopatologi Indonesia Vol. 8 No. 1 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

A number of Ganoderma spp. isolates was isolated from oil palms attacked by basal stem rot (BSR) disease in Padang and Pontianak plantations. Genetic polymorphism of these isolates was analyzed based on the internal transcribed spacer (ITS) sequence of ribosomal DNA region. In addition, a restriction fragment length polymorphism (RFLP) analysis was also performed to determine the association of the isolates with BSR disease. The isolated Ganoderma spp. showed high DNA polymorphism and there was no obvious genetic clustering of isolates that may correspond to their geographical position in Padang and Pontianak. This indicated that exchange of DNA between Ganoderma spp. infecting oil palm is not uncommon. This can be explained by the heterothallic nature of Ganoderma spp. in which DNA recombination occurs during sexual reproduction between different thalli. RFLP analysis showed that ITS fragments from all Ganoderma spp. isolates were digested with restriction enzymes MluI and SacI. This indicated that the anoderma spp. isolates were specific for oil palm and thus associated with the BSR.Key words: basal stem rot, Ganoderma spp., internal transcribed spacer, oil palm

Identifikasi Meloidogyne Penyebab Penyakit Umbi Bercabang pada Wortel di Dataran Tinggi Dieng Muhamad Taher; Supramana .; Gede Suastika
Jurnal Fitopatologi Indonesia Vol. 8 No. 1 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Branched tuber disease is a new problem on carrot cultivation in Indonesia. The disease may reduce the quantity and quality of the tubers and causing significant yield losses. Meloidogyne spp. has been identified as the primary cause of branched or forked disease of carrot in West Java. Similar disease was observed in carrot field in Dieng Plateau, Central Java. The study was conducted at three locations in the Dieng Plateau having different elevation. Nematode species identification was done by observing the perinneal pattern of 150 samples of female nematode and obtained four Meloidogyne species, namely M. arenaria, M. hapla, M. incognita, and M. javanica. All four species were found associated with branched tuber disease in three locations with M. incognita as a dominant species having prevalence levels of 50% to 58%.Key words: branched tuber disease, carrots, Meloidogyne, species identification

Preparasi RNA Virus Mosaik Bergaris dari Tanaman Tebu Menggunakan Metode Tabung PCR Tri Asmira Damayanti; Lilik Koesmihartono Putra
Jurnal Fitopatologi Indonesia Vol. 8 No. 1 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Sugarcane streak mosaic virus (SCSMV) is a new emerging virus infecting sugarcane in Indonesia. The virus can be found almost in every sugarcane plantations in Java and it was known as sett-borne virus. Attempt to get virus-free seedling meet difficulties due to lacking an easy, and accurate detection method. SCSMV antiserum is not available yet commercially. Nucleic acid detection by RT-PCR also hampered difficulties in releasing RNA virus from sugarcane tissues. Here we reported the simple direct tube method with minor modification as the convenient way to provide total RNA template from infected sugarcane leaf, stalk and sheath for RT-PCR detection of SCSMV.Key words: RNA extraction, RT-PCR, SCSMV, tube PCR

Penyakit Keriting Daun Pepaya yang Disebabkan oleh Cladosporium cladosporioides Widodo .; Suryo Wiyono
Jurnal Fitopatologi Indonesia Vol. 8 No. 1 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Leaf curling and chlorotic spot on young leaves of papaya type California was observed in Bogor, Kebumen, and Bekasi. Conidium of Cladosporium cladosporioides was found associated with diseased tissue based on microscopic examination. To our knowledge, this is the first report of C. cladosporioides causing papaya leaf curling in Indonesia. Key words: chlorotic, leaf curl disease, papaya disease, scab

Evaluasi Tiga Metode Preparasi RNA Total untuk Deteksi Turnip mosaic potyvirus dari Benih Brassica rappa dengan Reverse Transcription-Polymerase Chain Reaction Jati Adiputra; Sri Hendrastuti Hidayat; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Turnip mosaic virus (TuMV) is an important seedborne virus that infects vegetable crops, especially Brassicae group, in Indonesia. Reliable detection method having high accuracy is required to detect the virus from seeds in order to avoid disease incidence as early as possible. A study was conducted to evaluate three total RNA extraction methods from Brassica seeds for TuMV detection using reverse transcription- polymerase chain reaction. The samples used are Brassica rappa seeds and seedling germinated for 3, 5, and 7 days. Total RNA extraction methods evaluated consisted of Wylie method, Randles method, and commercial kit as comparation. Total RNA extracted using Wylie and Randles methods has high concentration but low level of purity, on the other hand total RNA obtained using commercial kit has low concentration but high level of purity. Detection of TuMV from seed was successfully carried out using Wylie and Randles methods, TuMV from 3 - day old seedlings was detected using all three methodes, TuMV from 5-day old seedlings was only detected using Randles method, whereas TuMV from 7-day old seedlings was not detected using all three methodes. Total RNA extraction method using Wylie and Randles methodes is recommended for detection of TuMV from caisin seed using RT-PCR technique. Key words: Brassica rappa seed, reverse transcription- polymerase chain reaction, Total RNA, Turnip mosaic potyvirus

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